| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05798-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98796-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99421-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97674-50ul | |||
![Western Blot: CRIPTO Antibody (JE48-67) [NBP3-32220] - Western blot analysis of CRIPTO on NCCIT cell lysates with Rabbit anti-CRIPTO antibody (NBP3-32220) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32220) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32220_rabbit-cripto-mab-je48-67-278202415295576.jpg "Western Blot: CRIPTO Antibody (JE48-67) [NBP3-32220] - Western blot analysis of CRIPTO on NCCIT cell lysates with Rabbit anti-CRIPTO antibody (NBP3-32220) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32220) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: RNF7 Antibody [NBP2-88166] - Host: Rabbit. Target Name: RBX2. Sample Type: 293T Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/RNF7-Antibody-Western-Blot-NBP2-88166-img0001.jpg "Western Blot: RNF7 Antibody [NBP2-88166] - Host: Rabbit. Target Name: RBX2. Sample Type: 293T Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: NRAS Antibody (06) [NBP3-05798] - NBP3-05798 at 1:500 dilution. Lane A: A431 Whole Cell Lysate Lane B: Mouse brain Tissue Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa](http://images.novusbio.com/fullsize/nbp3-05798_mouse-monoclonal-nras-antibody-06-23520251544333.png "Western Blot: NRAS Antibody (06) [NBP3-05798] - NBP3-05798 at 1:500 dilution. Lane A: A431 Whole Cell Lysate Lane B: Mouse brain Tissue Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa")
![Western Blot: GFI-1 Antibody [NBP2-82228] - WB Suggested Anti-GFI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Lung](http://images.novusbio.com/fullsize/GFI-1-Antibody-Western-Blot-NBP2-82228-img0001.jpg "Western Blot: GFI-1 Antibody [NBP2-82228] - WB Suggested Anti-GFI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Lung")
![Western Blot: ZBTB4 Antibody [NBP2-86403] - WB Suggested Anti-ZBTB4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Spleen](http://images.novusbio.com/fullsize/ZBTB4-Antibody-Western-Blot-NBP2-86403-img0001.jpg "Western Blot: ZBTB4 Antibody [NBP2-86403] - WB Suggested Anti-ZBTB4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Spleen")
![Western Blot: ZBTB4 Antibody [NBP2-86404] - WB Suggested Anti-ZBTB4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 293T cell lysate](http://images.novusbio.com/fullsize/ZBTB4-Antibody-Western-Blot-NBP2-86404-img0001.jpg "Western Blot: ZBTB4 Antibody [NBP2-86404] - WB Suggested Anti-ZBTB4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 293T cell lysate")
![Western Blot: FBXO6 Antibody [NBP2-98796] - Anti-FBXO6 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 37 kDa](http://images.novusbio.com/fullsize/FBXO6-Antibody-Western-Blot-NBP2-98796-img0001.jpg "Western Blot: FBXO6 Antibody [NBP2-98796] - Anti-FBXO6 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 37 kDa")
![Western Blot: NF2/Merlin Antibody [NBP2-99421] - Anti-NF2/Merlin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP2-99421-img0002.jpg "Western Blot: NF2/Merlin Antibody [NBP2-99421] - Anti-NF2/Merlin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa (We are unsure as to the identity of these extra bands).")
![Western Blot: Speedy/Ringo Antibody [NBP2-88357] - WB Suggested Anti-SPDYA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Western-Blot-NBP2-88357-img0001.jpg "Western Blot: Speedy/Ringo Antibody [NBP2-88357] - WB Suggested Anti-SPDYA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart")
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody [NBP2-97674] - Immunofluorescence staining of Skp2 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97674 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97674_rabbit-polyclonal-skp2-antibody-23520252135159.png "Immunocytochemistry/Immunofluorescence: Skp2 Antibody [NBP2-97674] - Immunofluorescence staining of Skp2 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97674 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry: p53 Antibody (PD01-32) [NBP3-32699] - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-p53 antibody (NBP3-32699) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32699) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32699_rabbit-p53-mab-pd01-32-298202413585511.jpg "Immunohistochemistry: p53 Antibody (PD01-32) [NBP3-32699] - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-p53 antibody (NBP3-32699) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32699) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: NPM1 [p Thr199] Antibody (JE63-16) [NBP3-32780] - Western blot analysis of NPM1 on different lysates with Rabbit anti-NPM1 antibody (NBP3-32780) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate Lane 3: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32780) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32780_rabbit-npm1-p-thr199-mab-je63-16-28820241244291.jpg "Western Blot: NPM1 [p Thr199] Antibody (JE63-16) [NBP3-32780] - Western blot analysis of NPM1 on different lysates with Rabbit anti-NPM1 antibody (NBP3-32780) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate Lane 3: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32780) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")