| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73985 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03913-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35827-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98877-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98989-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98787-50ul | |||
![Western Blot: Miz-1/ZBTB17 Antibody (HL1548) - Azide and BSA Free [NBP3-25565] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Miz1 antibody [HL1548] (NBP3-25565) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25565_rabbit-miz-1-zbtb17-mab-hl1548-azide-and-bsa-free-2120249374939.jpg "Western Blot: Miz-1/ZBTB17 Antibody (HL1548) - Azide and BSA Free [NBP3-25565] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Miz1 antibody [HL1548] (NBP3-25565) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: Miz-1/ZBTB17 Antibody (HL1548) - Azide and BSA Free [NBP3-25565] - Miz1 antibody [HL1548] detects Miz1 protein by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Miz1 stained by Miz1 antibody [HL1548] (NBP3-25565) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25565_rabbit-miz-1-zbtb17-mab-hl1548-azide-and-bsa-free-2120249422690.jpg)
![Western Blot: SAP102 Antibody (3F2) [NBP2-03428] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAP102 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAP102.](http://images.novusbio.com/fullsize/SAP102-Antibody-3F2-Western-Blot-NBP2-03428-img0002.jpg "Western Blot: SAP102 Antibody (3F2) [NBP2-03428] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAP102 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAP102.")
![Flow Cytometry: SAP102 Antibody (3F2) [NBP2-03428] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SAP102 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SAP102-Antibody-3F2-Flow-Cytometry-NBP2-03428-img0001.jpg)
![Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of lysates from Rat heart, using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33221_rabbit-uba3-mab-2d2j6-91220241512139.jpg "Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of lysates from Rat heart, using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of various lysates using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33221_rabbit-uba3-mab-2d2j6-912202414123886.jpg)
![Western Blot: LATS2 Antibody [NBP3-03913] - Analysis of extracts of various cell lines, using LATS2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/LATS2-Antibody-Western-Blot-NBP3-03913-img0004.jpg "Western Blot: LATS2 Antibody [NBP3-03913] - Analysis of extracts of various cell lines, using LATS2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CEND1 Antibody [NBP3-35827] - Western blot analysis of various lysates using CEND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35827_rabbit-cend1-pab-1012202411334026.jpg "Western Blot: CEND1 Antibody [NBP3-35827] - Western blot analysis of various lysates using CEND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: CEND1 Antibody [NBP3-35827] - Western blot analysis of various lysates using CEND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35827_rabbit-cend1-pab-1012202411404065.jpg)
![Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of various cell lines, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-4C5C8-Western-Blot-NBP3-16309-img0001.jpg "Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of various cell lines, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of Rat lung, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-4C5C8-Western-Blot-NBP3-16309-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DP1 Antibody [NBP3-10877] - Immunofluorescence of HCT116 cells using NBP3-10877 at a dilution of 4ug/mL. Secondary antibody: Anti-rabbit Alexa 546 at a dilution of 2ug/mL.](http://images.novusbio.com/fullsize/DP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-10877-img0001.jpg "Immunocytochemistry/Immunofluorescence: DP1 Antibody [NBP3-10877] - Immunofluorescence of HCT116 cells using NBP3-10877 at a dilution of 4ug/mL. Secondary antibody: Anti-rabbit Alexa 546 at a dilution of 2ug/mL.")
![Western Blot: Cyclin D3 Antibody [NBP2-98877] - Anti-Cyclin D3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-Western-Blot-NBP2-98877-img0002.jpg "Western Blot: Cyclin D3 Antibody [NBP2-98877] - Anti-Cyclin D3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa")
![Immunocytochemistry/Immunofluorescence: Cyclin D3 Antibody [NBP2-98877] - Immunofluorescence staining of Cyclin D3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98877 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98877_rabbit-polyclonal-cyclin-d3-antibody-23520251125446.png)
![Immunohistochemistry-Paraffin: Cdk3 Antibody [NBP2-98989] - Immunochemical staining of human Cdk3 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cdk3-Antibody-Immunohistochemistry-Paraffin-NBP2-98989-img0002.jpg "Immunohistochemistry-Paraffin: Cdk3 Antibody [NBP2-98989] - Immunochemical staining of human Cdk3 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunocytochemistry/Immunofluorescence: Cdk3 Antibody [NBP2-98989] - Immunofluorescence staining of Cdk3 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98989 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98989_rabbit-polyclonal-cdk3-antibody-235202511444415.png)
![Western Blot: E2F-4 Antibody [NBP2-98787] - Lane A: K562 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:53 kDa](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-NBP2-98787-img0002.jpg "Western Blot: E2F-4 Antibody [NBP2-98787] - Lane A: K562 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:53 kDa")
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody [NBP2-98787] - Immunofluorescence staining of E2F-4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98787 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-98787_rabbit-polyclonal-e2f-4-antibody-235202511444414.png)
![Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/USP16-Antibody-Western-Blot-NB100-81638-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for IP at 3 mcg/mg lysate. USP16 was also immunoprecipitated by rabbit anti- USP16 antibody NB100-81639, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/USP16-Antibody-Western-Blot-NB100-81638-img0001.jpg)
![Western Blot: PLK2 Antibody - BSA Free [NBP3-47114] - HeLa cells were subjected to SDS PAGE followed by western blot with (PLK2 antibody) at dilution of 1:2000](http://images.novusbio.com/fullsize/nbp3-47114_rabbit-plk2-pab-bsa-free-28420251231293.jpg "Western Blot: PLK2 Antibody - BSA Free [NBP3-47114] - HeLa cells were subjected to SDS PAGE followed by western blot with (PLK2 antibody) at dilution of 1:2000")
![Immunoprecipitation: PLK2 Antibody - BSA Free [NBP3-47114] - IP Result of anti-PLK2 (IP:, 4ug; Detection: 1:300) with HeLa cells lysate 2480ug.](http://images.novusbio.com/fullsize/nbp3-47114_rabbit-plk2-pab-bsa-free-284202512275021.jpg)