| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34679-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35188-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92622-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35904-20ul | |||
![Western Blot: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097] - Western blot analysis of VIPR1/VPAC1 on different lysates with Rabbit anti-VIPR1/VPAC1 antibody (NBP3-33097) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: MDA-MB-231 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: LNCaP cell lysate (20 ug/Lane) Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33097) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33097_rabbit-vipr1-vpac1-mab-pd00-96-288202414191544.jpg "Western Blot: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097] - Western blot analysis of VIPR1/VPAC1 on different lysates with Rabbit anti-VIPR1/VPAC1 antibody (NBP3-33097) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: MDA-MB-231 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: LNCaP cell lysate (20 ug/Lane) Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33097) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: VIPR1/VPAC1 Antibody (PD00-96) [NBP3-33097] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-VIPR1/VPAC1 antibody (NBP3-33097) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33097) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33097_rabbit-vipr1-vpac1-mab-pd00-96-298202414304187.jpg)
![Western Blot: Ninein Antibody [NB100-74631] - DGAT1 inhibition decreases ncMTOC-related proteins in prostate cancer cells. gamma-tubulin (48 kD), ninein (243 kD), GM130 (140 kD), and CETN1 (20 kD) levels were analyzed by western blot in PC-3 control (CTR) and treated with 1 uM DGAT1 inhibitor or 10 nM PEDF. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-39537-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ninein-Antibody-Western-Blot-NB100-74631-img0003.jpg "Western Blot: Ninein Antibody [NB100-74631] - DGAT1 inhibition decreases ncMTOC-related proteins in prostate cancer cells. gamma-tubulin (48 kD), ninein (243 kD), GM130 (140 kD), and CETN1 (20 kD) levels were analyzed by western blot in PC-3 control (CTR) and treated with 1 uM DGAT1 inhibitor or 10 nM PEDF. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-39537-z), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Ninein Antibody [NB100-74631] - Detection of Human and Mouse NINEIN on HeLa whole cell lysate using NB100-74631. NINEIN was also immunoprecipitated by another rabbit anti-NINEIN antibody.](http://images.novusbio.com/fullsize/Ninein-Antibody-Western-Blot-NB100-74631-img0002.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48669_rabbit-mttfa-mab-hl2012-western-blot-1411202518395418.jpg "<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>mtTFA antibody [HL2012] detects mtTFA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.mtTFA stained by mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48669_rabbit-mttfa-mab-hl2012-immunohistochemistry-paraffin-141120251839548.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48669_rabbit-mttfa-mab-hl2012-western-blot-1411202518322913.jpg)
![Somatostatin R4/SSTR4 antibody [HL2933] detects Somatostatin R4/SSTR4 protein by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.Somatostatin R4/SSTR4 stained by Somatostatin R4/SSTR4 antibody [HL2933] (NBP3-48752) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48752_rabbit-somatostatin-r4-sstr4-mab-hl2933-immunohistochemistry-paraffin-1411202518321612.jpg "Somatostatin R4/SSTR4 antibody [HL2933] detects Somatostatin R4/SSTR4 protein by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.Somatostatin R4/SSTR4 stained by Somatostatin R4/SSTR4 antibody [HL2933] (NBP3-48752) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin R4/SSTR4 antibody [HL2933] (NBP3-48752) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48752_rabbit-somatostatin-r4-sstr4-mab-hl2933-western-blot-1411202518431313.jpg)
![<b>Orthogonal Strategies Validation. </b>Unboiled Non-transfected (-) and transfected (+) 293T and Expi293 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin R4/SSTR4 antibody [HL2933] (NBP3-48752) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48752_rabbit-somatostatin-r4-sstr4-mab-hl2933-western-blot-1411202518324114.jpg)
![Western Blot: Ext2 Antibody [NBP1-58297] - Human Hela, Antibody Dilution: 1.0 ug/ml EXT2 is supported by BioGPS gene expression data to be expressed in HeLa.](http://images.novusbio.com/fullsize/Ext2-Antibody-Western-Blot-NBP1-58297-img0003.jpg "Western Blot: Ext2 Antibody [NBP1-58297] - Human Hela, Antibody Dilution: 1.0 ug/ml EXT2 is supported by BioGPS gene expression data to be expressed in HeLa.")
![Immunohistochemistry: Ext2 Antibody [NBP1-58297] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: N/A Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/Ext2-Antibody-Immunohistochemistry-NBP1-58297-img0004.jpg)
![Western Blot: Ext2 Antibody [NBP1-58297] - 721_B cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Ext2-Antibody-Western-Blot-NBP1-58297-img0002.jpg)
![Western Blot: PP5 Antibody [NBP3-35188] - Western blot analysis of various lysates using PP5 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35188_rabbit-pp5-pab-912202414112897.jpg "Western Blot: PP5 Antibody [NBP3-35188] - Western blot analysis of various lysates using PP5 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Immunocytochemistry/ Immunofluorescence: PP5 Antibody [NBP3-35188] - Immunofluorescence analysis of HeLa cells using PP5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35188_rabbit-pp5-pab-31120251744424.jpg%3Ft=1708655913)
![Immunocytochemistry/Immunofluorescence: CDK5 Activator 1 Antibody [NBP2-92622] - Analysis of SH-SY5Y cells using CDK5 Activator 1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92622-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDK5 Activator 1 Antibody [NBP2-92622] - Analysis of SH-SY5Y cells using CDK5 Activator 1 at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: CDK5 Activator 1 Antibody - Azide and BSA Free [NBP2-92622] - Western blot analysis of various lysates, using CDK5 Activator 1 antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92622_rabbit-polyclonal-cdk5-activator-1-antibody-422025143186.jpg%3Ft=1690973940)
![Immunocytochemistry/ Immunofluorescence: Ext2 Antibody [NBP3-35904] - Immunofluorescence analysis of L929 cells using Ext2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35904_rabbit-ext2-pab-912202414201328.jpg "Immunocytochemistry/ Immunofluorescence: Ext2 Antibody [NBP3-35904] - Immunofluorescence analysis of L929 cells using Ext2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: Ext2 Antibody [NBP3-35904] - Western blot analysis of various lysates using Ext2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35904_rabbit-ext2-pab-91220241418110.jpg)
![Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/IRS2-Antibody-Immunohistochemistry-Paraffin-NB100-68244-img0002.jpg "Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: IRS2 Antibody [NB100-68244] - Detection of Human IRS2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IRS2 antibody NB100-68244 used for IP at 3 mcg/mg lysate. IRS2 was also immunoprecipitated by rabbit anti-IRS2 antibodies NB100-68242 and NB100-68243, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/IRS2-Antibody-Immunoprecipitation-NB100-68244-img0003.jpg)
![Western Blot: SGTA Antibody [NBP2-20339] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SGTA-Antibody-Western-Blot-NBP2-20339-img0002.jpg "Western Blot: SGTA Antibody [NBP2-20339] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: SGTA Antibody [NBP2-20339] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/SGTA-Antibody-Western-Blot-NBP2-20339-img0001.jpg)