| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92848-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92872-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38567-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35288-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92653-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-10535-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11884-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98781-50ul | |||
![Western Blot: Cullin 3 Antibody [NBP2-92848] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Cullin 3 Antibody-Western Blot-NBP2-92848-img0002.jpg "Western Blot: Cullin 3 Antibody [NBP2-92848] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Cullin 3 Antibody [NBP2-92848] - Analysis of HeLa cells using Cullin 3 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92848-img0001.jpg)
![Western Blot: Cullin 3 Antibody [NBP2-92872] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NBP2-92872-img0002.jpg "Western Blot: Cullin 3 Antibody [NBP2-92872] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Cullin 3 Antibody [NBP2-92872] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NBP2-92872-img0001.jpg)
![Western Blot: TCP1-beta Antibody (9N6B4) [NBP3-16465] - Western blot analysis of extracts of various cell lines, using TCP1-beta Rabbit mAb (NBP3-16465) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-9N6B4-Western-Blot-NBP3-16465-img0002.jpg "Western Blot: TCP1-beta Antibody (9N6B4) [NBP3-16465] - Western blot analysis of extracts of various cell lines, using TCP1-beta Rabbit mAb (NBP3-16465) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry-Paraffin: TCP1-beta Antibody (9N6B4) [NBP3-16465] - Immunohistochemistry of paraffin-embedded rat brain using TCP1-beta Rabbit mAb (NBP3-16465) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-9N6B4-Immunohistochemistry-Paraffin-NBP3-16465-img0001.jpg)
![Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CDC25C-Antibody-6R0I6-Western-Blot-NBP3-15437-img0002.jpg "Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (6R0I6) [NBP3-15437] - Immunofluorescence analysis of HeLa cells using CDC25C Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15437_rabbit-cdc25c-mab-6r0i6-72202512435382.jpg)
![Western Blot: BCCIP Antibody [NBP3-38567] - Western blot analysis of various lysates using BCCIP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38567_rabbit-bccip-pab-912202415171153.jpg "Western Blot: BCCIP Antibody [NBP3-38567] - Western blot analysis of various lysates using BCCIP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunohistochemistry: BCCIP Antibody [NBP3-38567] - Immunohistochemistry analysis of paraffin-embedded Human breast using BCCIP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38567_rabbit-bccip-pab-912202415175244.jpg)
![Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-10122024120667.jpg "Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk7 Antibody [NBP3-35288] - Western Blot analysis of lysates from wild type (WT) and Cdk7 knockout (KO) 293T cells, using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-31120251744483.jpg%3Ft=1709093634)
![Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts of various cell lines, using CETN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Centrin-2-Antibody-Western-Blot-NBP2-92653-img0003.jpg "Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts of various cell lines, using CETN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts from normal (control) and CETN2 knockout (KO) HeLa cells, using CETN2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Centrin-2-Antibody-Knockout-Validated-NBP2-92653-img0005.jpg)
![Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/CDC7-Antibody-DCS-341-Western-Blot-NB120-10535-img0001.jpg "Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Immunohistochemistry: Bcl-6 Antibody (A6C7-R) [NBP3-32074] - Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-Bcl-6 antibody (NBP3-32074) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32074) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32074_mouse-bcl-6-mab-a6c7-r-29820241420947.jpg "Immunohistochemistry: Bcl-6 Antibody (A6C7-R) [NBP3-32074] - Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-Bcl-6 antibody (NBP3-32074) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32074) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody (A6C7-R) [NBP3-32074] - Immunocytochemistry analysis of Raji cells labeling Bcl-6 with Mouse anti-Bcl-6 antibody (NBP3-32074) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Bcl-6 antibody (NBP3-32074) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32074_mouse-bcl-6-mab-a6c7-r-2920241114839.jpg)
![Immunocytochemistry/Immunofluorescence: p19 INK4d Antibody (DCS100) [NBP3-11884] - Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age. Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color). The blue color is a fluorescent nuclear probe, TOPRO3. (Image modified from original in citation 1).](http://images.novusbio.com/fullsize/p19-INK4d-Antibody-DCS100-Immunocytochemistry-Immunofluorescence-NBP3-11884-img0001.jpg "Immunocytochemistry/Immunofluorescence: p19 INK4d Antibody (DCS100) [NBP3-11884] - Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age. Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color). The blue color is a fluorescent nuclear probe, TOPRO3. (Image modified from original in citation 1).")
![Western Blot: NCAPH Antibody [NBP2-98781] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:82 kDaObserved band size:110 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP2-98781-img0001.jpg "Western Blot: NCAPH Antibody [NBP2-98781] - Lane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:82 kDaObserved band size:110 kDa")
![Immunoprecipitation: NCAPH Antibody [NBP2-98781] - Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-NCAPH rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NCAPH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 83 kDaObserved band size: 110 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunoprecipitation-NBP2-98781-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP2-98781] - Staining of NCAPH in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-98781 antibody (1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98781_rabbit-polyclonal-ncaph-antibody-23520251135415.png)
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414161884.jpg "Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32600) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414333686.jpg)
![Immunocytochemistry/ Immunofluorescence: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunocytochemistry analysis of A431 cells labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-292024111485.jpg)