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![Western Blot: LATS1 Antibody [NBP1-58271] - THP-1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/LATS1-Antibody-Western-Blot-NBP1-58271-img0002.jpg "Western Blot: LATS1 Antibody [NBP1-58271] - THP-1 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: MKLP1 Antibody (9V4Y8) [NBP3-16507] - Western blot analysis of extracts of various cell lines, using MKLP1 Rabbit mAb (NBP3-16507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MKLP1-Antibody-9V4Y8-Western-Blot-NBP3-16507-img0001.jpg "Western Blot: MKLP1 Antibody (9V4Y8) [NBP3-16507] - Western blot analysis of extracts of various cell lines, using MKLP1 Rabbit mAb (NBP3-16507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunoprecipitation: MKLP1 Antibody (9V4Y8) [NBP3-16507] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug MKLP1 antibody . Western blot was performed from the immunoprecipitate using MKLP1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-16507_rabbit-mklp1-mab-9v4y8-7220251245328.jpg)
![Immunoprecipitation:[NBP3-30591]-Detection of human Septin 2 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-Septin 2 antibody used for IP at 6 µg/mg lysate. Septin 2 was also immunoprecipitated by rabbit anti-Septin 2 antibody. For blotting immunoprecipitated Septin 2, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30591_rabbit-septin-2-pab-2312025128864.jpg "Immunoprecipitation:[NBP3-30591]-Detection of human Septin 2 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-Septin 2 antibody used for IP at 6 µg/mg lysate. Septin 2 was also immunoprecipitated by rabbit anti-Septin 2 antibody. For blotting immunoprecipitated Septin 2, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunoprecipitation:Septin-2 AntibodyNBP3-30511] -Detection of human Septin 2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Septin 2 antibody NBP3-30511 (lot NBP3-30511-1) used for IP at 6 µg/mg lysate. Septin 2 was also immunoprecipitated by rabbit anti-Septin 2.. For blotting immunoprecipitated Septin 2, NBP3-30511 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30511_rabbit-septin-2-pab-3012202412262521.jpg "Immunoprecipitation:Septin-2 AntibodyNBP3-30511] -Detection of human Septin 2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Septin 2 antibody NBP3-30511 (lot NBP3-30511-1) used for IP at 6 µg/mg lysate. Septin 2 was also immunoprecipitated by rabbit anti-Septin 2.. For blotting immunoprecipitated Septin 2, NBP3-30511 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot: PARD3B Antibody [NBP1-97771] (0.5ug/ml) staining of Mouse fetal Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PARD3B-Antibody-Western-Blot-NBP1-97771-img0002.jpg "Western Blot: PARD3B Antibody [NBP1-97771] (0.5ug/ml) staining of Mouse fetal Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Septin-2 Antibody (9N1B3) [NBP3-16653] - Western blot analysis of extracts of various cell lines, using Septin-2 Rabbit mAb (NBP3-16653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Septin-2-Antibody-9N1B3-Western-Blot-NBP3-16653-img0001.jpg "Western Blot: Septin-2 Antibody (9N1B3) [NBP3-16653] - Western blot analysis of extracts of various cell lines, using Septin-2 Rabbit mAb (NBP3-16653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Western-Blot-NBP1-74027-img0005.jpg "Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B")
![Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of Aurora B in LNCap cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Flow-Cytometry-NBP1-74027-img0006.jpg)
![Immunoprecipitation: CDC2L2 Antibody [NB100-495] - Detection of human CDC2L2 (CDK11) by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CDK11 antibody NB100-495 (lot 2) used for WB at 1 ug/ml (A) and used for IP at 6 ug/mg lysate. CDK11 was also immunoprecipitated by a previous lot (lot 1) of this antibody and by rabbit anti-CDK11 antibody NB100-496, which recognizes a downstream epitope. For blotting immunoprecipitated CDK11, NB100-496 was used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](http://images.novusbio.com/fullsize/CDC2L2-Antibody-Immunoprecipitation-NB100-495-img0001.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: CDC2L2 Antibody [NB100-495] - Detection of human CDC2L2 (CDK11) by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CDK11 antibody NB100-495 (lot 2) used for WB at 1 ug/ml (A) and used for IP at 6 ug/mg lysate. CDK11 was also immunoprecipitated by a previous lot (lot 1) of this antibody and by rabbit anti-CDK11 antibody NB100-496, which recognizes a downstream epitope. For blotting immunoprecipitated CDK11, NB100-496 was used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).")
![Western Blot: Aurora B Antibody (AURKB/1521) [NBP2-53267] - Western Blot Analysis (A) Recombinant Protein (B) Human Liver Lysate Using Aurora B Monoclonal Antibody (AURKB/1521).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-AURKB-1521-Western-Blot-NBP2-53267-img0001.jpg "Western Blot: Aurora B Antibody (AURKB/1521) [NBP2-53267] - Western Blot Analysis (A) Recombinant Protein (B) Human Liver Lysate Using Aurora B Monoclonal Antibody (AURKB/1521).")
![Western Blot: Astrin Antibody [NB100-74639] - Detection of Human Astrin/MAP126 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74639 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74638 , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/Astrin-Antibody-Western-Blot-NB100-74639-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Astrin Antibody [NB100-74639] - Detection of Human Astrin/MAP126 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74639 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74638 , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")