| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38021-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37989-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81058-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26502-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21943-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22415-50ul | |||
![Immunohistochemistry: EAAT3 Antibody [NBP3-38021] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using SLC1A1 Rabbit pAb at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38021_rabbit-eaat3-pab-10122024140439.jpg "Immunohistochemistry: EAAT3 Antibody [NBP3-38021] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using SLC1A1 Rabbit pAb at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: EAAT3 Antibody [NBP3-38021] - Western blot analysis of various lysates using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38021_rabbit-eaat3-pab-10122024140454.jpg)
![Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody [NBP3-37989] - Immunofluorescence analysis of U2OS cells using CTBP2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37989_rabbit-ctbp2-pab-10122024121033.jpg "Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody [NBP3-37989] - Immunofluorescence analysis of U2OS cells using CTBP2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: CTBP2 Antibody [NBP3-37989] - Western blot analysis of various lysates using CTBP2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-37989_rabbit-ctbp2-pab-101220241233858.jpg)
![Western Blot: 14-3-3 gamma Antibody (5R4Q4) [NBP3-16771] - Western blot analysis of extracts of various cell lines, using 14-3-3 gamma Rabbit mAb (NBP3-16771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-5R4Q4-Western-Blot-NBP3-16771-img0002.jpg "Western Blot: 14-3-3 gamma Antibody (5R4Q4) [NBP3-16771] - Western blot analysis of extracts of various cell lines, using 14-3-3 gamma Rabbit mAb (NBP3-16771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: 14-3-3 gamma Antibody (5R4Q4) [NBP3-16771] - Immunohistochemistry of paraffin-embedded mouse brain using 14-3-3 gamma Rabbit mAb (NBP3-16771) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-5R4Q4-Immunohistochemistry-Paraffin-NBP3-16771-img0001.jpg)
![Western Blot: Cdk5 Antibody (6O8F8) [NBP3-33430] - Western blot analysis of various lysates, using [KO Validated] Cdk5 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33430_rabbit-cdk5-mab-6o8f8-912202414163021.jpg "Western Blot: Cdk5 Antibody (6O8F8) [NBP3-33430] - Western blot analysis of various lysates, using [KO Validated] Cdk5 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk5 Antibody (6O8F8) [NBP3-33430] - Western blot analysis of lysates from wild type(WT) and Cdk5 knockout (KO) 293T cells, using [KO Validated] Cdk5 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33430_rabbit-cdk5-mab-6o8f8-52202511411411.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk5 Antibody (6O8F8) [Cdk5] - Western blot analysis of lysates from wild type(WT) and Cdk5 knockout (KO) 293T cells, using [KO Validated] Cdk5 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33430_rabbit-cdk5-mab-6o8f8-52202517352724.jpg)
![Western Blot: CENPF Antibody (PSH0-79) [NBP3-32181] - Western blot analysis of CENPF on different lysates with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 358 kDa Observed band size: 358 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32181) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32181_rabbit-cenpf-mab-psh0-79-278202416331287.jpg "Western Blot: CENPF Antibody (PSH0-79) [NBP3-32181] - Western blot analysis of CENPF on different lysates with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 358 kDa Observed band size: 358 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32181) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CENPF Antibody (PSH0-79) [NBP3-32181] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32181) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32181_rabbit-cenpf-mab-psh0-79-298202414262657.jpg)
![Western Blot: Septin-5 Antibody (SP18) [NBP2-81058] - Western Blot using [NBP2-81058]. Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81058 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa CDCrel-1 protein were detected in rat brain and mouse spinal chord. In this experiment no CDCrel-1 was dected in the other samples.](http://images.novusbio.com/fullsize/Septin-5-Antibody-SP18-Western-Blot-NBP2-81058-img0001.jpg "Western Blot: Septin-5 Antibody (SP18) [NBP2-81058] - Western Blot using [NBP2-81058]. Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81058 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa CDCrel-1 protein were detected in rat brain and mouse spinal chord. In this experiment no CDCrel-1 was dected in the other samples.")
![Immunohistochemistry: Septin-5 Antibody (SP18) [NBP2-81058] - Immunohistochemical staining of rabbit brain using [NBP2-81058]. Formalin fixed rabbit brain slices were were stained with NBP2-81058 at 3 ug/ml.](http://images.novusbio.com/fullsize/Septin-5-Antibody-SP18-Immunohistochemistry-NBP2-81058-img0002.jpg)
![Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: APP Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa Observed band size: 100 kDa](http://images.novusbio.com/fullsize/nbp3-26502_rabbit-app-mab-6f10-282202411413611.jpg "Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: APP Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa Observed band size: 100 kDa")
![Immunocytochemistry/Immunofluorescence: APP Antibody (6F10) [NBP3-26502] - Staining of Hela Cells with APP Antibody (6F10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26502_rabbit-app-mab-6f10-28220241312218.jpg)
![Western Blot: CaM Kinase II Antibody (SR1956) [NBP3-21943] - Western blot analysis using NBP3-21943 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21943_rabbit-cam-kinase-ii-mab-sr1956-57202316494275.jpg "Western Blot: CaM Kinase II Antibody (SR1956) [NBP3-21943] - Western blot analysis using NBP3-21943 in HeLa cell lysate.")
![Western Blot: CTBP2 Antibody (SR1810) [NBP3-22415] - Western blot analysis using NBP3-22415 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22415_rabbit-ctbp2-mab-sr1810-57202315545139.jpg "Western Blot: CTBP2 Antibody (SR1810) [NBP3-22415] - Western blot analysis using NBP3-22415 in HeLa cell lysate.")
![Western Blot: BRSK1 Antibody - BSA Free [NBP3-47852] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (BRSK1 antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-47852_rabbit-brsk1-pab-bsa-free-28420251133320.jpg "Western Blot: BRSK1 Antibody - BSA Free [NBP3-47852] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (BRSK1 antibody) at dilution of 1:500")
![Immunohistochemistry: BRSK1 Antibody - BSA Free [NBP3-47852] - Immunohistochemistry of paraffin-embedded human ovary tumor using (BRSK1 antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47852_rabbit-brsk1-pab-bsa-free-28420251134734.jpg)
![Immunohistochemistry-Paraffin: Septin-5 Antibody [NBP3-12342] - Mouse Brain. 1:50 dilution in IHC blocking buffer. DAB (brown) staining Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/Septin-5-Antibody-Immunohistochemistry-Paraffin-NBP3-12342-img0001.jpg "Immunohistochemistry-Paraffin: Septin-5 Antibody [NBP3-12342] - Mouse Brain. 1:50 dilution in IHC blocking buffer. DAB (brown) staining Hematoxylin QS (blue) counterstain. 40X magnification.")
![Western Blot: p38 gamma/SAPK3 Antibody (R13-L) [NBP3-08207] - Western blot analysis of p38 gamma/SAPK3. Lane A - human recombinant 38-alpha(260-360a.a., N-terminal GST tag; H00001432-Q01), lane B - human recombinant p38-beta(with C-terminal DDK tag, NBL1-12870), lane C - human recombinant p38 gamma/SAPK3 (1-368a.a., N-terminal GST tag, H00006300-P01), lane D - human recombinant p38-delta (1-366a.a., N-terminal GST tag, H00005603-P01). 500ng of recombinant protein was loaded in each well.](http://images.novusbio.com/fullsize/p38-gamma-SAPK3-Antibody-R13-L-Western-Blot-NBP3-08207-img0001.jpg "Western Blot: p38 gamma/SAPK3 Antibody (R13-L) [NBP3-08207] - Western blot analysis of p38 gamma/SAPK3. Lane A - human recombinant 38-alpha(260-360a.a., N-terminal GST tag; H00001432-Q01), lane B - human recombinant p38-beta(with C-terminal DDK tag, NBL1-12870), lane C - human recombinant p38 gamma/SAPK3 (1-368a.a., N-terminal GST tag, H00006300-P01), lane D - human recombinant p38-delta (1-366a.a., N-terminal GST tag, H00005603-P01). 500ng of recombinant protein was loaded in each well.")
![Immunocytochemistry/Immunofluorescence: p38 gamma/SAPK3 Antibody (R13-L) [NBP3-08207] - Representative picture of p38 gamma/SAPK3 expression in HEK293 cells, visualized with clonal rabbit anti-p38 gamma/SAPK3 monospecific antibody. Primary antibody dilution - 1:300.](http://images.novusbio.com/fullsize/p38-gamma-SAPK3-Antibody-R13-L-Immunocytochemistry-Immunofluorescence-NBP3-08207-img0002.jpg)