| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97553-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44221 | |||
| BSA Free | NBP3-07986-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91782-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32614-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49443-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35190-20ul | |||
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0002.jpg)
![ICC/IF: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp3-13144_rabbit-tor-mtor-p-ser-2448-pab-immunocytochemistry-immunofluorescence-126202482926..jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP2-97553] - Anti-Use1/UBE2Z rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP2-97553-img0003.jpg "Western Blot: Use1/UBE2Z Antibody [NBP2-97553] - Anti-Use1/UBE2Z rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa")
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP2-97553] - Immunochemical staining of human Use1/UBE2Z in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP2-97553-img0004.jpg)
![Immunoprecipitation: Use1/UBE2Z Antibody [NBP2-97553] - Use1/UBE2Z was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-Use1/UBE2Z rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Use1/UBE2Z rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunoprecipitation-NBP2-97553-img0001.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody - BSA Free [NBP2-97553] - Immunochemical staining of human Use1/UBE2Z in human duodenum with NBP2-97553 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-97553_rabbit-polyclonal-use1-ube2z-antibody-23520251220550.png)
![Immunocytochemistry/Immunofluorescence: Use1/UBE2Z Antibody [NBP2-97553] - Immunofluorescence staining of Use1/UBE2Z in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97553 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97553_rabbit-polyclonal-use1-ube2z-antibody-235202512205520.png)
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0005.jpg "Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab")
![Immunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of paraffin-embedded mouse placenta tissue using anti-Tuberin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Immunohistochemistry-Paraffin-NBP2-67552-img0002.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Flow cytometric analysis of TSC2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0004.jpg)
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Tuberin on Jurkat cell lysates using anti-Tuberin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0003.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Hela cells with Tuberin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0001.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cerebral cortex and liver tissues using NBP3-07986 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cerebral cortex and liver tissues using NBP3-07986 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Western-Blot-NBP3-07986-img0008.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human cerebral cortex shows strong cytoplasmic positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0003.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0004.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglia and nerves.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0005.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of rat brain shows strong cytoplasmic positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0006.jpg)
![Western Blot: CHMP4B Antibody [NBP1-91782] - Analysis in control (vector only transfected HEK293T lysate) and CHMP4B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP1-91782-img0013.jpg "Western Blot: CHMP4B Antibody [NBP1-91782] - Analysis in control (vector only transfected HEK293T lysate) and CHMP4B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP1-91782] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP1-91782-img0012.jpg)
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP1-91782] - Staining of human liver shows weak granular cytoplasmic positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP1-91782-img0008.jpg)
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP1-91782] - Staining of human small intestine shows moderate to strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP1-91782-img0010.jpg)
![Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Analysis in human placenta and liver tissues using NBP2-32614 antibody. Corresponding FOXO4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Analysis in human placenta and liver tissues using NBP2-32614 antibody. Corresponding FOXO4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human cerebellum shows no positivity in purkinje cells as expected.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0001.jpg)
![Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0002.jpg)
![Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human testis shows moderate to strong nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0003.jpg)
![Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0004.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining in human skin and pancreas tissues using anti-UBA3 antibody. Corresponding UBA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining in human skin and pancreas tissues using anti-UBA3 antibody. Corresponding UBA3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: UBA3 Antibody [NBP2-49443] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-49443-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: UBA3 Antibody [NBP2-49443] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & centrosome.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49443-img0004.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0006.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0005.jpg)
![Western Blot: UBA3 Antibody [NBP2-48628] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-48628-img0002.jpg "Western Blot: UBA3 Antibody [NBP2-48628] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG")
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human testis shows strong nuclear positivity.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0007.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human cerebellum shows moderate nuclear positivity in purkinje cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0004.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0005.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human skin shows moderate nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0006.jpg)
![Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-[p-Ser78]-Antibody-Western-Blot-NBP2-24458-img0001.jpg "Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-25153-img0001.jpg "Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25153] - HeLa cells stained with NBP2-25153 (red) counterstained with monoclonal antibody to Hsp27, NBP2-25149 (green) and DNA (blue). NBP2-25153 reveals strong nuclear lamina staining, while NBP2-25149 reveals strong cytoplasmic staining. Since both DNA (blue) and Lamin A + C (red) are associated with the nuclear compartment, this region appears crimson in this image.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25153-img0003.jpg)
![Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Western-Blot-NBP2-32263-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunohistochemistry-NBP2-32263-img0004.jpg)
![Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunohistochemistry-NBP2-32263-img0003.jpg)
![Immunoprecipitation: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by rabbit anti-NAT10 antibody BL15900. For blotting immunoprecipitated NAT10, NBP2-32263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunoprecipitation-NBP2-32263-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: NAT10 Antibody [NBP2-32263] - Detection of human NAT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by another rabbit anti-NAT10 antibody. For blotting immunoprecipitated NAT10, NBP2-32263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunoprecipitation-NBP2-32263-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP3-35190] - Immunofluorescence analysis of NIH/3T3 cells using Calpain 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35190_rabbit-calpain-1-pab-912202415175220.jpg "Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP3-35190] - Immunofluorescence analysis of NIH/3T3 cells using Calpain 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: Calpain 1 Antibody [NBP3-35190] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Calpain 1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35190_rabbit-calpain-1-pab-912202414154360.jpg)
![Western Blot: Calpain 1 Antibody [NBP3-35190] - Western blot analysis of various lysates using Calpain 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35190_rabbit-calpain-1-pab-9122024159527.jpg)
![Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP3-35190] - Immunofluorescence analysis of HeLa cells using Calpain 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35190_rabbit-calpain-1-pab-9122024159598.jpg)