| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48126 | |||
| Azide and BSA Free | NBP3-11598 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-642AFP488 | |||
| Alexa Fluor Plus 555 | NB600-642AFP555 | |||
| Alexa Fluor Plus 594 | NB600-642AFP594 | |||
| Alexa Fluor Plus 647 | NB600-642AFP647 | |||
| Alexa Fluor Plus 680 | NB600-642AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47766-0.1mg | |||
| IHC-Prediluted | NBP2-48133 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50040SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47768-0.1mg | |||
| IHC-Prediluted | NBP2-48153 | |||
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (SPM619) [NBP2-44688] - Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cyclin B1 MAb (SPM619)followed by Goat anti-mouse IgG-CF488 (Green).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-SPM619-Immunocytochemistry-Immunofluorescence-NBP2-44688-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (SPM619) [NBP2-44688] - Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cyclin B1 MAb (SPM619)followed by Goat anti-mouse IgG-CF488 (Green).")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) [NBP2-44688] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (SPM619)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-SPM619-Immunohistochemistry-Paraffin-NBP2-44688-img0001.jpg)
![Western Blot: Thymidylate Synthase Antibody [NB600-642] - Detection thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB substrate for approximately 4'.](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-Western-Blot-NB600-642-img0003.jpg "Western Blot: Thymidylate Synthase Antibody [NB600-642] - Detection thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB substrate for approximately 4'.")
![Western Blot: Thymidylate Synthase Antibody [NB600-642] - Detection thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB substrate for approximately 4'.](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-Western-Blot-NB600-642-img0004.jpg)
![Immunohistochemistry-Paraffin: p57 Kip2 Antibody (KIP2/880) [NBP2-44490] - Human Prostate Carcinoma stained with p57 Monoclonal Antibody (KIP2/880).](http://images.novusbio.com/fullsize/p57-Kip2-Antibody-KIP2-880-Immunohistochemistry-Paraffin-NBP2-44490-img0002.jpg "Immunohistochemistry-Paraffin: p57 Kip2 Antibody (KIP2/880) [NBP2-44490] - Human Prostate Carcinoma stained with p57 Monoclonal Antibody (KIP2/880).")
![Immunohistochemistry-Paraffin: p57 Kip2 Antibody (KIP2/880) [NBP2-44490] - Human Colon Carcinoma stained with p57 Monoclonal Antibody (KIP2/880).](http://images.novusbio.com/fullsize/p57-Kip2-Antibody-KIP2-880-Immunohistochemistry-Paraffin-NBP2-44490-img0001.jpg)
![Immunohistochemistry-Paraffin: GPS2 Antibody [NBP2-14970] - Human gastric cancer, using GPS2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GPS2-Antibody-Immunohistochemistry-Paraffin-NBP2-14970-img0001.jpg "Immunohistochemistry-Paraffin: GPS2 Antibody [NBP2-14970] - Human gastric cancer, using GPS2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GPS2 Antibody [NBP2-14970] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-14970_rabbit-polyclonal-gps2-antibody-1610202419414529.jpg)
![Western Blot: NAT10 Antibody [NBP2-15034] - Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NAT10-Antibody-Western-Blot-NBP2-15034-img0003.jpg "Western Blot: NAT10 Antibody [NBP2-15034] - Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: NAT10 Antibody [NBP2-15034] - Paraformaldehyde-fixed MCF-7, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15034-img0001.jpg)
![Immunohistochemistry-Paraffin: NAT10 Antibody [NBP2-15034] - Paraffin-embedded SCC4 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunohistochemistry-Paraffin-NBP2-15034-img0002.jpg)
![Western Blot: MKLP1 Antibody [NBP2-17299] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17299_rabbit-polyclonal-mklp1-antibody-91020242012200.jpg "Western Blot: MKLP1 Antibody [NBP2-17299] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: MKLP1 Antibody [NBP2-17299] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17299_rabbit-polyclonal-mklp1-antibody-91020242001044.jpg)
![MKLP1 antibody [N1N2], N-term detects MKLP1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.](http://images.novusbio.com/fullsize/nbp2-17299_rabbit-polyclonal-mklp1-antibody-immunocytochemistry-immunofluorescence-261120251943811.jpg)
![MKLP1 antibody [N1N2], N-term detects MKLP1 protein at midbody by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-17299_rabbit-polyclonal-mklp1-antibody-immunocytochemistry-immunofluorescence-261120251995416.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB600-472] - Shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel).](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Western-Blot-NB600-472-img0004.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB600-472] - Shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel).")
![Immunohistochemistry: Ubiquitin-activating Enzyme/UBE1 Antibody [NB600-472] - Used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Immunohistochemistry-NB600-472-img0006.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Lane 1: unstimulated A431 cells. Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min). Load: 30 ug per lane. Primary antibody: EGFR pY1197 antibody at 1:250 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 134.3 kDa, ~170 kDa for EGFR pY1197.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-[p-Tyr1197]-Antibody-Western-Blot-NBP1-77936-img0003.jpg "Western Blot: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Lane 1: unstimulated A431 cells. Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min). Load: 30 ug per lane. Primary antibody: EGFR pY1197 antibody at 1:250 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 134.3 kDa, ~170 kDa for EGFR pY1197.")
![Immunohistochemistry-Paraffin: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Tissue: placental trophoblasts. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: EGFR pY1197 antibody at 5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: EGFR pY1197 is on the cell membrane. Staining: EGFR pY1197 as precipitated red signal with hematoxylin purple counterstain.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-[p-Tyr1197]-Antibody-Immunohistochemistry-Paraffin-NBP1-77936-img0002.jpg)
![Western Blot: COP9 Antibody [NBP2-15956] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/COP9-Antibody-Western-Blot-NBP2-15956-img0001.jpg "Western Blot: COP9 Antibody [NBP2-15956] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: COP9 Antibody [NBP2-15956] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COP9 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/COP9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15956-img0003.jpg)
![Immunohistochemistry-Paraffin: COP9 Antibody [NBP2-15956] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/COP9-Antibody-Immunohistochemistry-Paraffin-NBP2-15956-img0002.jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Western-Blot-NBP1-71840-img0007.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71840] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti- EBP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunohistochemistry-NBP1-71840-img0008.jpg)
![Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1A11-Western-Blot-NBP2-50040-img0005.jpg "Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1A11) [NBP2-50040] - HeLa cell cultures stained with NBP2-50040 (green). Aurora A localizes in spindle poles and mitotic spindles at late mitosis. Counterstained is chicken polyclonal antibody against Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1A11-Immunocytochemistry-Immunofluorescence-NBP2-50040-img0003.jpg)
![Immunohistochemistry-Paraffin: p57 Kip2 Antibody (57P06) [NBP2-44492] - Human Prostate Carcinoma stained with p57 Kip2 Antibody (KIP2/880).](http://images.novusbio.com/fullsize/p57-Kip2-Antibody-57P06-Immunohistochemistry-Paraffin-NBP2-44492-img0002.jpg "Immunohistochemistry-Paraffin: p57 Kip2 Antibody (57P06) [NBP2-44492] - Human Prostate Carcinoma stained with p57 Kip2 Antibody (KIP2/880).")