| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38156-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03659-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38168-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93026-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06560-50ul | |||
![Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP3-38156] - Immunofluorescence analysis of BALB-3T3 cells using UBE2R1/CDC34 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38156_rabbit-ube2r1-cdc34-pab-912202414201358.jpg "Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP3-38156] - Immunofluorescence analysis of BALB-3T3 cells using UBE2R1/CDC34 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: UBE2R1/CDC34 Antibody [NBP3-38156] - Western blot analysis of various lysates using UBE2R1/CDC34 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38156_rabbit-ube2r1-cdc34-pab-91220241418145.jpg)
![Western Blot: UBE2R1/CDC34 Antibody [NBP3-38156] - Western blot analysis of lysates from Mouse testis, using UBE2R1/CDC34 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38156_rabbit-ube2r1-cdc34-pab-9122024158120.jpg)
![Western Blot: KRAS Antibody [NBP3-03659] - Analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/KRAS-Antibody-Knockout-Validated-NBP3-03659-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: KRAS Antibody [NBP3-03659] - Analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Polyclonal KRAS Antibody [NBP3-03659] - Analysis of various lysates, using KRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/antibody/nbp3-03659_rabbit-polyclonal-kras-antibody-western-blot-542023103522.jpg)
![Immunocytochemistry/ Immunofluorescence: KRAS Antibody - Azide and BSA Free [NBP3-03659] - Immunofluorescence analysis of L929 cells using [KO Validated] KRAS Rabbit pAb (A12704) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03659_rabbit-polyclonal-kras-antibody-52202519302990.jpg)
![Western Blot: GLIPR1L2 Antibody [NBP2-82092] - Analysis of GLIPR1L2 in human testis tissue lysate with GLIPR1L2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/GLIPR1L2-Antibody-Western-Blot-NBP2-82092-img0001.jpg "Western Blot: GLIPR1L2 Antibody [NBP2-82092] - Analysis of GLIPR1L2 in human testis tissue lysate with GLIPR1L2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: GLIPR1L2 Antibody [NBP2-82092] - Immunofluorescence of GLIPR1L2 in human testis tissue with GLIPR1L2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/GLIPR1L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82092-img0003.jpg)
![Immunohistochemistry: GLIPR1L2 Antibody [NBP2-82092] - Immunohistochemistry of GLIPR1L2 in human testis tissue with GLIPR1L2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/GLIPR1L2-Antibody-Immunohistochemistry-NBP2-82092-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-38168] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-91220241581241.jpg "Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-38168] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunoprecipitation: MCM5 Antibody [NBP3-38168] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug MCM5 antibody. Western blot was performed from the immunoprecipitate using MCM5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-912202415111841.jpg)
![Western Blot: MCM5 Antibody [NBP3-38168] - Western blot analysis of various lysates using MCM5 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-912202414112858.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Cell lysates of 293T(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-k1G6-Western-Blot-NBP1-04337-img0005.jpg "Western Blot: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Cell lysates of 293T(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Analysis in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1G antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-k1G6-Immunocytochemistry-Immunofluorescence-NBP1-04337-img0006.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-k1G6-Immunohistochemistry-Paraffin-NBP1-04337-img0004.jpg)
![Western Blot: TOB2 Antibody [NBP2-93026] - Analysis of extracts of various cell lines, using TOB2 . Exposure time: 30s.](http://images.novusbio.com/fullsize/TOB2-Antibody-Western-Blot-NBP2-93026-img0003.jpg "Western Blot: TOB2 Antibody [NBP2-93026] - Analysis of extracts of various cell lines, using TOB2 . Exposure time: 30s.")
![Immunohistochemistry-Paraffin: TOB2 Antibody [NBP2-93026] - Paraffin-embedded mouse lung using TOB2 .](http://images.novusbio.com/fullsize/TOB2-Antibody-Immunohistochemistry-Paraffin-NBP2-93026-img0002.jpg)
![Immunohistochemistry-Paraffin: TOB2 Antibody [NBP2-93026] - Paraffin-embedded human liver damage using TOB2 .](http://images.novusbio.com/fullsize/TOB2-Antibody-Immunohistochemistry-Paraffin-NBP2-93026-img0001.jpg)
![Western Blot: DTYMK Antibody (JE64-08) [NBP3-32280] - Western blot analysis of DTYMK on different lysates with Rabbit anti-DTYMK antibody (NBP3-32280) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 24 kDa Observed band size: 18 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32280) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32280_rabbit-dtymk-mab-je64-08-27820241631693.jpg "Western Blot: DTYMK Antibody (JE64-08) [NBP3-32280] - Western blot analysis of DTYMK on different lysates with Rabbit anti-DTYMK antibody (NBP3-32280) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 24 kDa Observed band size: 18 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32280) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DTYMK Antibody (JE64-08) [NBP3-32280] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-DTYMK antibody (NBP3-32280) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32280) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32280_rabbit-dtymk-mab-je64-08-29820241420937.jpg)
![Immunocytochemistry/ Immunofluorescence: DTYMK Antibody (JE64-08) [NBP3-32280] - Immunocytochemistry analysis of NCI-H441 cells labeling DTYMK with Rabbit anti-DTYMK antibody (NBP3-32280) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-DTYMK antibody (NBP3-32280) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32280_rabbit-dtymk-mab-je64-08-29202410234229.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Anti-CDC7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-Paraffin-NLS7979-img0003.jpg "Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Anti-CDC7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Analysis of anti-CDC7 antibody with testis at 5.2 ug/ml.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-NLS7979-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Analysis of anti-CDC7 antibody with human ovary, carcinoma.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-Paraffin-NLS7979-img0002.jpg)
![Western Blot: UBE2R1/CDC34 Antibody [NBP3-06560] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-Western-Blot-NBP3-06560-img0001.jpg "Western Blot: UBE2R1/CDC34 Antibody [NBP3-06560] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa")
![Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody [NBP3-06560] - Staining of human CDC34 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-Immunohistochemistry-Paraffin-NBP3-06560-img0002.jpg)
![Immunoprecipitation: UBE2R1/CDC34 Antibody [NBP3-06560] - Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-CDC34 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CDC34 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :26 kDa](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-Immunoprecipitation-NBP3-06560-img0003.jpg)
![Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-27820241631678.jpg "Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32237) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-29820241435260.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunocytochemistry analysis of HeLa cells labeling Cyclin E2 with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-292024119231.jpg)
![Western Blot: CIAO1 Antibody [NBP2-84682] - Host: Rabbit. Target Name: CIAO1. Sample Type: MCF7. Antibody Dilution: 1.0ug/mlCIAO1 is supported by BioGPS gene expression data to be expressed in MCF7](http://images.novusbio.com/fullsize/CIAO1-Antibody-Western-Blot-NBP2-84682-img0003.jpg "Western Blot: CIAO1 Antibody [NBP2-84682] - Host: Rabbit. Target Name: CIAO1. Sample Type: MCF7. Antibody Dilution: 1.0ug/mlCIAO1 is supported by BioGPS gene expression data to be expressed in MCF7")
![Immunohistochemistry-Paraffin: CIAO1 Antibody [NBP2-84682] - Rabbit Anti-CIAO1 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue. Observed Staining: Cytoplasmic in alveolar type I cells. Primary Antibody Concentration: 1:100. Other Working Concentrations: 1/600. Secondary Antibody: Donkey anti-Ra](http://images.novusbio.com/fullsize/CIAO1-Antibody-Immunohistochemistry-Paraffin-NBP2-84682-img0001.jpg)
![Western Blot: CIAO1 Antibody [NBP2-84682] - WB Suggested Anti-CIAO1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysateCIAO1 is supported by BioGPS gene expression data to be expressed in 721_B](http://images.novusbio.com/fullsize/CIAO1-Antibody-Western-Blot-NBP2-84682-img0002.jpg)
![Western Blot: CIAO1 Antibody [NBP2-84682] - Host: Rabbit. Target Name: CIAO1. Sample Type: 721_B. Antibody Dilution: 1.0ug/mlCIAO1 is supported by BioGPS gene expression data to be expressed in 721_B](http://images.novusbio.com/fullsize/CIAO1-Antibody-Western-Blot-NBP2-84682-img0004.jpg)