| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26278-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56369SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56736SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-120SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97931SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21579-50ul | |||
![Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-2622024132372.jpg "Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).")
![Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-26220241447153.jpg)
![Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-2722024856319.jpg)
![Western Blot: Topoisomerase I Antibody (6D8) [NBP3-26278] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, PC-3 whole cell lysate. All lanes: Topoisomerase I Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 91 kDa Observed band size: 91 kDa](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-28220241144269.jpg)
![Flow Cytometry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Overlay histogram showing HepG2 cells stained with Topoisomerase I Antibody (6D8) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-282202414312734.jpg)
![Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0001.jpg "Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.")
![Western Blot: ANP32B Antibody [NB100-56369] - analysis of APRIL in HL-60 whole cell lysate using APRIL antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0002.jpg)
![Western Blot: RGS2 Antibody [NB100-56736] - Western bot analysis of RGS2 in human heart lysate using RGS2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/RGS2-Antibody-Western-Blot-NB100-56736-img0002.jpg "Western Blot: RGS2 Antibody [NB100-56736] - Western bot analysis of RGS2 in human heart lysate using RGS2 antibody at 2 ug/ml.")
![Western Blot: Somatostatin Receptor 1 Antibody [NB300-120] - Detection of SSR1 from rat pancreas using NB300-120 (1:1000).](http://images.novusbio.com/fullsize/Somatostatin-Receptor-1-Antibody-Western-Blot-NB300-120-img0003.jpg "Western Blot: Somatostatin Receptor 1 Antibody [NB300-120] - Detection of SSR1 from rat pancreas using NB300-120 (1:1000).")
![Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-19925] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PRC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19925-img0003.jpg "Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-19925] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-19925] - SCM1 xenograft, using PRC1antibody at 1:100 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/PRC1-Antibody-Immunohistochemistry-Paraffin-NBP2-19925-img0002.jpg)
![Western Blot: PRC1 Antibody [NBP2-19925] - Sample (30 ug of whole cell lysate) A: JurKat B: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PRC1-Antibody-Western-Blot-NBP2-19925-img0001.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PRC1 antibody [N1N2], N-term (NBP2-19925) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19925_rabbit-polyclonal-prc1-antibody-western-blot-2611202518333610.jpg)
![Western Blot: RABGAP1 Antibody [NBP2-15086] - Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Western-Blot-NBP2-15086-img0004.jpg "Western Blot: RABGAP1 Antibody [NBP2-15086] - Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE; antibody diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: RABGAP1 Antibody [NBP2-15086] - Analysis of methanol-fixed HeLa, using RABGAP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15086-img0001.jpg)
![Immunohistochemistry-Paraffin: RABGAP1 Antibody [NBP2-15086] - Paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-15086-img0002.jpg)
![Western Blot: RABGAP1 Antibody [NBP2-15086] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Western-Blot-NBP2-15086-img0003.jpg)
![Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-271-img0004.jpg "Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat ATM antibody [NB100-271] used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, ATM antibody [NB100-271] was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunoprecipitation-NB100-271-img0005.jpg)
![Immunohistochemistry: PIF1 Antibody [NBP1-97931] - IHC analysis of PIF1 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PIF1-Antibody-Immunohistochemistry-NBP1-97931-img0001.jpg "Immunohistochemistry: PIF1 Antibody [NBP1-97931] - IHC analysis of PIF1 in mouse brain using DAB with hematoxylin counterstain.")
![Western Blot: Septin-9 Antibody [NBP1-28764] - Hela whole cell lysate (5, 15 and 50ug) probed with NBP1-28764 at 0.04ug/ml.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP1-28764-img0001.jpg "Western Blot: Septin-9 Antibody [NBP1-28764] - Hela whole cell lysate (5, 15 and 50ug) probed with NBP1-28764 at 0.04ug/ml.")
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP1-28764] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Septin 9 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-NBP1-28764-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Septin-9 Antibody [NBP1-28764] - IP of 1mg of Hela whole cell lysate, 20% of IP loaded. Lane 1, IP using NBP1-28764. Lane 2, IP using NBP1-28711 and lane 3, IP using control IgG. Western blot probed with NBP1-28764 at 0.4ug/ml.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunoprecipitation-NBP1-28764-img0002.jpg)
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)
![Western Blot: DLG5 Antibody [NBP1-28739] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DLG5 antibody NBP1-28739 used for WB at 0.1 ug/mL (A) and 1 ug/mL (B) and used for IP at 10 ug/mg lysate. DLG5 was also immunoprecipitated by rabbit anti-DLG5 antibody NBP1-28694, which recognizes an upstream epitope. Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/DLG5-Antibody-Western-Blot-NBP1-28739-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DLG5 Antibody [NBP1-28739] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DLG5 antibody NBP1-28739 used for WB at 0.1 ug/mL (A) and 1 ug/mL (B) and used for IP at 10 ug/mg lysate. DLG5 was also immunoprecipitated by rabbit anti-DLG5 antibody NBP1-28694, which recognizes an upstream epitope. Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DLG5 Antibody [NBP1-28739] - IP of overexpressed CitK (citron-kinase) in HEK293 cell line. DLG5 is co-immunoprecipitated with the DLG5 antibody but not with control beads or with a control isotypic antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DLG5-Antibody-Immunoprecipitation-NBP1-28739-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Immunofluorescent analysis of Jurkat cells treated with Pervanadate, using NBP3-21579.](http://images.novusbio.com/fullsize/nbp3-21579_rabbit-jak2-p-tyr-1007-mab-sr1129-5720231526114.jpg "Immunocytochemistry/Immunofluorescence: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Immunofluorescent analysis of Jurkat cells treated with Pervanadate, using NBP3-21579.")
![Immunohistochemistry: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Immunohistochemical analysis of paraffin-embedded human cervix cancer, using NBP3-21579.](http://images.novusbio.com/fullsize/nbp3-21579_rabbit-jak2-p-tyr-1007-mab-sr1129-57202315545129.jpg)
![Western Blot: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Western blot analysis using NBP3-21579 in Jurkat cell lysates treated with Pervanadate.](http://images.novusbio.com/fullsize/nbp3-21579_rabbit-jak2-p-tyr-1007-mab-sr1129-5720231649421.jpg)