| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08389 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38168-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79863 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79878 | |||
![Western Blot: MCM2 Antibody (JE42-39) [NBP3-32577] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Mouse thymus tissue lysate (40 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: C2C12 cell lysate (20 ug/Lane) Lane 9: U-87 MG cell lysate (20 ug/Lane) Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32577_rabbit-mcm2-mab-je42-39-278202415212456.jpg "Western Blot: MCM2 Antibody (JE42-39) [NBP3-32577] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Mouse thymus tissue lysate (40 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: C2C12 cell lysate (20 ug/Lane) Lane 9: U-87 MG cell lysate (20 ug/Lane) Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM2 Antibody (JE42-39) [NBP3-32577] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32577) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32577_rabbit-mcm2-mab-je42-39-298202414262652.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (JE42-39) [NBP3-32577] - Immunocytochemistry analysis of HeLa cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/250 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32577_rabbit-mcm2-mab-je42-39-29202410484867.jpg)
![Western Blot: MCM3 Antibody [NBP1-33028] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NBP1-33028-img0005.jpg "Western Blot: MCM3 Antibody [NBP1-33028] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody [NBP1-33028] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM3 protein stained by MCM3 antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33028-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM3 Antibody [NBP1-33028] - Paraffin-embedded NCIN87 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-Paraffin-NBP1-33028-img0004.jpg)
![Western Blot: MCM7 Antibody (rMCM7/1468) [NBP3-07277] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 cell lysates using MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-rMCM7-1468-Western-Blot-NBP3-07277-img0003.jpg "Western Blot: MCM7 Antibody (rMCM7/1468) [NBP3-07277] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 cell lysates using MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (rMCM7/1468) [NBP3-07277] - Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-rMCM7-1468-Immunohistochemistry-Paraffin-NBP3-07277-img0002.jpg)
![Protein Array: MCM7 Antibody (rMCM7/1468) [NBP3-07277] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-rMCM7-1468-Protein-Array-NBP3-07277-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-38168] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-91220241581241.jpg "Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-38168] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunoprecipitation: MCM5 Antibody [NBP3-38168] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug MCM5 antibody. Western blot was performed from the immunoprecipitate using MCM5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-912202415111841.jpg)
![Western Blot: MCM5 Antibody [NBP3-38168] - Western blot analysis of various lysates using MCM5 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-912202414112858.jpg)
![Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0059.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM3 Antibody [NB100-249] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified goat anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-NB100-249-img0061.jpg)
![Western Blot: MCM3 Antibody [NB100-249] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0002.jpg)
![Western Blot: CDC45L Antibody [NBP2-15834] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC45L antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Western-Blot-NBP2-15834-img0004.jpg "Western Blot: CDC45L Antibody [NBP2-15834] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC45L antibody diluted at 1:1000.")
![Western Blot: CDC45L Antibody [NBP2-15834] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Western-Blot-NBP2-15834-img0001.jpg)
![CDC45L antibody [N3C3] detects CDC45L protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDC45L protein stained by CDC45L antibody [N3C3] (NBP2-15834) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15834_rabbit-polyclonal-cdc45l-antibody-immunocytochemistry-immunofluorescence-261120251826136.jpg)
![Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0066.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM6 Antibody [NB100-251] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM6-Antibody-Immunohistochemistry-NB100-251-img0068.jpg)
![Western Blot: MCM6 Antibody [NB100-251] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0002.jpg)
![Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887] - Western blot detection of MCM2 in Hela, A549, HL-60, U2OS cell lysates using NBP3-19887 (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.](http://images.novusbio.com/fullsize/MCM2-Antibody-S02-0D3-Western-Blot-NBP3-19887-img0003.jpg "Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887] - Western blot detection of MCM2 in Hela, A549, HL-60, U2OS cell lysates using NBP3-19887 (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.")
![Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunohistochemistry of MCM2 in paraffin-embedded Human tonsil using NBP3-19887 at dilution 1/50](http://images.novusbio.com/fullsize/MCM2-Antibody-S02-0D3-Immunohistochemistry-Paraffin-NBP3-19887-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunocytochemistry of MCM2 (green) in hela using NBP3-19887 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19887_rabbit-mcm2-mab-s02-0d3-113202515145446.jpg)
![Western Blot: MCM3 Antibody (3E1) [H00004172-M03] - MCM3 monoclonal antibody (M03), clone 3E1 Western Blot analysis of MCM3 expression in HeLa [L013V1].](http://images.novusbio.com/fullsize/MCM3-Antibody-3E1-Western-Blot-H00004172-M03-img0010.jpg "Western Blot: MCM3 Antibody (3E1) [H00004172-M03] - MCM3 monoclonal antibody (M03), clone 3E1 Western Blot analysis of MCM3 expression in HeLa [L013V1].")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (3E1) [H00004172-M03] - Analysis of monoclonal antibody to MCM3 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/MCM3-Antibody-3E1-Immunocytochemistry-Immunofluorescence-H00004172-M03-img0006.jpg)
![Western Blot: MCM3 Antibody (3E1) [H00004172-M03] - Analysis of MCM3 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/MCM3-Antibody-3E1-Western-Blot-H00004172-M03-img0009.jpg)
![Sandwich ELISA: MCM3 Antibody (3E1) [H00004172-M03] - Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM3-Antibody-3E1-Sandwich-ELISA-H00004172-M03-img0007.jpg)
![Western Blot: MCM6 Antibody (7D8) [H00004175-M04] - Analysis of MCM6 expression in transfected 293T cell line by MCM6 monoclonal antibody (M04), clone 7D8. Lane 1: MCM6 transfected lysate (Predicted MW: 92.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCM6-Antibody-7D8-Western-Blot-H00004175-M04-img0003.jpg "Western Blot: MCM6 Antibody (7D8) [H00004175-M04] - Analysis of MCM6 expression in transfected 293T cell line by MCM6 monoclonal antibody (M04), clone 7D8. Lane 1: MCM6 transfected lysate (Predicted MW: 92.9 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MCM6 Antibody (7D8) [H00004175-M04] - Analysis of monoclonal antibody to MCM6 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/MCM6-Antibody-7D8-Immunocytochemistry-Immunofluorescence-H00004175-M04-img0001.jpg)
![Sandwich ELISA: MCM6 Antibody (7D8) [H00004175-M04] - Detection limit for recombinant GST tagged MCM6 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM6-Antibody-7D8-Sandwich-ELISA-H00004175-M04-img0002.jpg)
![Immunohistochemistry-Paraffin: MCM6 Antibody (MCM6/3000) [NBP2-79728] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MCM6 Antibody (MCM6/3000).](http://images.novusbio.com/fullsize/MCM6-Antibody-MCM6-3000-Immunohistochemistry-Paraffin-NBP2-79728-img0001.jpg "Immunohistochemistry-Paraffin: MCM6 Antibody (MCM6/3000) [NBP2-79728] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MCM6 Antibody (MCM6/3000).")
![Protein Array: MCM6 Antibody (MCM6/3000) [NBP2-79728] - Analysis of Protein Array containing >19,000 full-length human proteins using MCM6 Antibody (MCM6/3000) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5](http://images.novusbio.com/fullsize/MCM6-Antibody-MCM6-3000-Protein-Array-NBP2-79728-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Geminin Antibody (CPTC-GMMN-1) [NBP2-79743] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Geminin with Geminin Antibody (CPTC-GMMN-1) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin](http://images.novusbio.com/fullsize/Geminin-Antibody-CPTC-GMMN-1-Immunocytochemistry-Immunofluorescence-NBP2-79743-img0001.jpg "Immunocytochemistry/Immunofluorescence: Geminin Antibody (CPTC-GMMN-1) [NBP2-79743] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Geminin with Geminin Antibody (CPTC-GMMN-1) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin")
![Protein Array: Geminin Antibody (CPTC-GMMN-1) [NBP2-79743] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Geminin Antibody (CPTC-GMMN-1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5](http://images.novusbio.com/fullsize/Geminin-Antibody-CPTC-GMMN-1-Protein-Array-NBP2-79743-img0002.jpg)