| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16891-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35251-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37326-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1701-0.025ml | |||
![Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Smad2-[p-Ser467]-Antibody-Western-Blot-NBP3-16891-img0001.jpg "Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Rat testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125711..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Mouse testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125830..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Human kidney tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125949..jpg)
![Western Blot: c-Myc Overexpression Lysate (Adult Normal) [NBL1-13414] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myc.](http://images.novusbio.com/fullsize/c-Myc-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13414-img0002.jpg "Western Blot: c-Myc Overexpression Lysate (Adult Normal) [NBL1-13414] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myc.")
![Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of L929 cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-1012202411592885.jpg "Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of L929 cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: MAD2L2 Antibody [NBP3-35251] - Western blot analysis of various lysates using MAD2L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-10122024120615.jpg)
![Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of U2OS cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-101220241243958.jpg)
![Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of H9C2 cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-101220241252827.jpg)
![Western Blot: COP9 Antibody (7V1L8) [NBP3-15791] - Western blot analysis of extracts of various cell lines, using COP9 Rabbit mAb (NBP3-15791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/COP9-Antibody-7V1L8-Western-Blot-NBP3-15791-img0004.jpg "Western Blot: COP9 Antibody (7V1L8) [NBP3-15791] - Western blot analysis of extracts of various cell lines, using COP9 Rabbit mAb (NBP3-15791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: COP9 Antibody (7V1L8) [COP9] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using COP9 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15791_rabbit-cop9-mab-7v1l8-5220251733592.jpg)
![Immunohistochemistry: COP9 Antibody (7V1L8) [COP9] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using COP9 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15791_rabbit-cop9-mab-7v1l8-52202517315543.jpg)
![Immunohistochemistry: COP9 Antibody (7V1L8) [COP9] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using COP9 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15791_rabbit-cop9-mab-7v1l8-5220251734541.jpg)
![Western Blot: EB3 Antibody (7J9V3) [NBP3-15904] - Western blot analysis of extracts of various cell lines, using EB3 Rabbit mAb (NBP3-15904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/EB3-Antibody-7J9V3-Western-Blot-NBP3-15904-img0004.jpg "Western Blot: EB3 Antibody (7J9V3) [NBP3-15904] - Western blot analysis of extracts of various cell lines, using EB3 Rabbit mAb (NBP3-15904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of U-2 OS cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112146..jpg)
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C6 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112350..jpg)
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C2C12 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112518..jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0004.jpg "Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.")
![Flow Cytometry: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Flow-Cytometry-NBP2-37326-img0005.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against human EGFR recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0002.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against HEK293 (1) and EGFR (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0003.jpg)
![ELISA: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-ELISA-NBP2-37326-img0001.jpg)
![Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Azide-and-BSA-Free-Western-Blot-NBP2-73184-img0001.jpg "Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.")
![Flow Cytometry: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by CDKN2B antibody.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73184-img0002.jpg)
![Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-Western-Blot-NB100-1701-img0007.jpg "Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1701-img0008.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Western-Blot-NBP1-44893-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Immunoprecipitation-NBP1-44893-img0002.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Lanes: 1: 50ug HEK293T lysate, 2: 50ug A549 lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:5000. Gene Name: SIAH2. Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena.](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0006.jpg "Western Blot: SIAH2 Antibody [NBP2-85730] - Lanes: 1: 50ug HEK293T lysate, 2: 50ug A549 lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:5000. Gene Name: SIAH2. Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena.")
![Western Blot: SIAH2 Antibody [NBP2-85730] - WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0001.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Type: HepG2. Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in HepG2](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0002.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Type: MCF7. Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in MCF7](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0003.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Tissue: Human OVCAR-3 Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0005.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Type: Hela. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide. Primary Antibody Concentration: 1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 0.12](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0004.jpg)
![Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-28820241419150.jpg "Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-UBA3 antibody (NBP3-33072) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29820241420944.jpg)
![Immunocytochemistry/ Immunofluorescence: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunocytochemistry analysis of SiHa cells labeling UBA3 with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29202410334093.jpg)
![Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-278202416285221.jpg "Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29820241420942.jpg)
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunocytochemistry analysis of HeLa cells treated with or without 50nM dexamethasone for 16 hours labeling p57 Kip2 with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29202410484891.jpg)