| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35251-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35473-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52519-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1701-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89858-50ul | |||
![Western Blot: Cadherin-13 Antibody (4Z8D1) [NBP3-16158] - Western blot analysis of extracts of Mouse heart, using Cadherin-13 Rabbit mAb (NBP3-16158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Cadherin-13-Antibody-4Z8D1-Western-Blot-NBP3-16158-img0004.jpg "Western Blot: Cadherin-13 Antibody (4Z8D1) [NBP3-16158] - Western blot analysis of extracts of Mouse heart, using Cadherin-13 Rabbit mAb (NBP3-16158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: Cadherin-13 Antibody (4Z8D1) [NBP3-16158] - Immunohistochemistry analysis of Cadherin-13 in paraffin-embedded mouse kidney tissue using Cadherin-13 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16158_rabbit-cadherin-13-mab-4z8d1-7220251245311.jpg)
![Immunohistochemistry: Cadherin-13 Antibody (4Z8D1) [NBP3-16158] - Immunohistochemistry analysis of Cadherin-13 in paraffin-embedded human cervix cancer tissue using Cadherin-13 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16158_rabbit-cadherin-13-mab-4z8d1-72202512432010.jpg)
![Immunohistochemistry: Cadherin-13 Antibody (4Z8D1) [NBP3-16158] - Immunohistochemistry analysis of Cadherin-13 in paraffin-embedded human colon carcinoma tissue using Cadherin-13 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16158_rabbit-cadherin-13-mab-4z8d1-7220251244332.jpg)
![Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of L929 cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-1012202411592885.jpg "Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of L929 cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: MAD2L2 Antibody [NBP3-35251] - Western blot analysis of various lysates using MAD2L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-10122024120615.jpg)
![Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of U2OS cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-101220241243958.jpg)
![Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of H9C2 cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-101220241252827.jpg)
![Western Blot: TLK1 Antibody [NBP3-35473] - Western blot analysis of various lysates using TLK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35473_rabbit-tlk1-pab-912202415161832.jpg "Western Blot: TLK1 Antibody [NBP3-35473] - Western blot analysis of various lysates using TLK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: TLK1 Antibody [NBP3-35473] - Immunohistochemistry analysis of paraffin-embedded Human placenta using TLK1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35473_rabbit-tlk1-pab-912202414163051.jpg)
![Immunohistochemistry: TLK1 Antibody [NBP3-35473] - Immunohistochemistry analysis of paraffin-embedded Rat heart using TLK1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35473_rabbit-tlk1-pab-91220241519535.jpg)
![Immunohistochemistry: TLK1 Antibody [NBP3-35473] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using TLK1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35473_rabbit-tlk1-pab-912202415133453.jpg)
![Western Blot: PPM1D Antibody [NBP2-81881] - Analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Western-Blot-NBP2-81881-img0001.jpg "Western Blot: PPM1D Antibody [NBP2-81881] - Analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: PPM1D Antibody - BSA Free [NBP2-81881] - Immunofluorescence of PPM1D in human lung carcinoma tissue with PPM1D antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-81881_rabbit-polyclonal-ppm1d-antibody-271120242074637.jpg)
![Immunohistochemistry: PPM1D Antibody - BSA Free [NBP2-81881] - Immunohistochemistry of PPM1D in human lung carcinoma tissue with PPM1D antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81881_rabbit-polyclonal-ppm1d-antibody-2711202419531610.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mouse mAb against Hela (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0005.jpg "Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mouse mAb against Hela (1), and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis of paraffin-embedded ovarian cancer tissues using NME2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Immunohistochemistry-Paraffin-NBP2-52519-img0004.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - nalysis using NME2 mAb against HEK293 (1) and NME2 (AA: FULL(1-152))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0003.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mAb against human NME2 (AA: FULL(1-152)) recombinant protein. (Expected MW is 43.2 kDa)](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0002.jpg)
![ELISA: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-ELISA-NBP2-52519-img0007.jpg)
![Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-Western-Blot-NB100-1701-img0007.jpg "Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1701-img0008.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Anti-PP2C gamma/PPM1g rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:59 kDa.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-112-Western-Blot-NBP2-89858-img0005.jpg "Western Blot: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Anti-PP2C gamma/PPM1g rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:59 kDa.")
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of human PPM1G in human epididymis with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-112-Immunohistochemistry-Paraffin-NBP2-89858-img0002.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of human PPM1G in human heart with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-112-Immunohistochemistry-Paraffin-NBP2-89858-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of Human PPM1G in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89858 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/nbp2-89858_rabbit-monoclonal-pp2c-gamma-ppm1g-antibody-112-23520251129355.png)
![Western Blot: CLK1 Antibody [NBP2-87194] - MCF-7 and HeLa whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. CLK1 Antibody (NBP2-87194) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CLK1-Antibody-Western-Blot-NBP2-87194-img0003.jpg "Western Blot: CLK1 Antibody [NBP2-87194] - MCF-7 and HeLa whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. CLK1 Antibody (NBP2-87194) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.")
![Western Blot: CLK1 Antibody [NBP2-87194] - WB Suggested Anti-CLK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysateCLK1 is supported by BioGPS gene expression data to be expressed in HEK293T](http://images.novusbio.com/fullsize/CLK1-Antibody-Western-Blot-NBP2-87194-img0001.jpg)
![Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-278202415242669.jpg "Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32579) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-298202413585583.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunocytochemistry analysis of SiHa cells labeling MCM6 with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-29202410234240.jpg)
![Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-278202415295570.jpg "Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody (JE64-91) [NBP3-32844] - Western blot analysis of PP2C gamma/PPM1G on different lysates with Rabbit anti-PP2C gamma/PPM1G antibody (NBP3-32844) at 1/500 dilution. Lane 1: 293T cell lysate, 10 ug/Lane Lane 2: Jurkat cell lysate, 10 ug/Lane Lane 3: Rat testis tissue lysate, 20 ug/Lane Predicted band size: 59 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32844) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32844_rabbit-pp2c-gamma-ppm1g-mab-je64-91-288202412461628.jpg "Western Blot: PP2C gamma/PPM1G Antibody (JE64-91) [NBP3-32844] - Western blot analysis of PP2C gamma/PPM1G on different lysates with Rabbit anti-PP2C gamma/PPM1G antibody (NBP3-32844) at 1/500 dilution. Lane 1: 293T cell lysate, 10 ug/Lane Lane 2: Jurkat cell lysate, 10 ug/Lane Lane 3: Rat testis tissue lysate, 20 ug/Lane Predicted band size: 59 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32844) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PP2C gamma/PPM1G Antibody (JE64-91) [NBP3-32844] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-PP2C gamma/PPM1G antibody (NBP3-32844) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32844) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32844_rabbit-pp2c-gamma-ppm1g-mab-je64-91-2982024142091.jpg)
![Flow Cytometry: PP2C gamma/PPM1G Antibody (JE64-91) [NBP3-32844] - Flow cytometric analysis of HepG2 cells labeling PP2C gamma/PPM1G. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32844, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32844_rabbit-pp2c-gamma-ppm1g-mab-je64-91-392024884672.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)