| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92733-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-667-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22415-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37363-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61823-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50390-0.025ml | |||
![Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of extracts of various cell lines, using COMP/Thrombospondin-5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/COMP-Thrombospondin-5-Antibody-Western-Blot-NBP2-92733-img0003.jpg "Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of extracts of various cell lines, using COMP/Thrombospondin-5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of L929 cells using COMP Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/COMP-Thrombospondin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92733-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of Human Dermal Fibroblasts (HDFa). Green: 1:100 COMP/Thrombospondin-5 antibody in PBS/BSA 1%, 1h room temperature Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-92733_rabbit-polyclonal-comp-thrombospondin-5-antibody-162023124536.jpg)
![Western Blot: Dicer Antibody (S167-7) [NBP2-22415] - Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Western-Blot-NBP2-22415-img0004.jpg "Western Blot: Dicer Antibody (S167-7) [NBP2-22415] - Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.")
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Dicer Antibody (D) Composite.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Immunocytochemistry-Immunofluorescence-NBP2-22415-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Dicer Antibody (D) Composite.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Immunocytochemistry-Immunofluorescence-NBP2-22415-img0003.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Western-Blot-NBP1-22410-img0005.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Immunocytochemistry-Immunofluorescence-NBP1-22410-img0006.jpg)
![Western Blot: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Analysis using FLT3 mAb against human FLT3 recombinant protein. (Expected MW is 32.6 kDa)](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7B7C3-Western-Blot-NBP2-37363-img0002.jpg "Western Blot: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Analysis using FLT3 mAb against human FLT3 recombinant protein. (Expected MW is 32.6 kDa)")
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Analysis of liver cancer tissues using FLT3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7B7C3-Immunohistochemistry-NBP2-37363-img0004.jpg)
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Analysis of cervical cancer tissues using FLT3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7B7C3-Immunohistochemistry-NBP2-37363-img0003.jpg)
![ELISA: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7B7C3-ELISA-NBP2-37363-img0001.jpg)
![Western Blot: BCL9-2 Antibody (3B9C1) [NBP2-61823] - Analysis using BCL9L mAb against human BCL9L (AA: 606-751) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/BCL9-2-Antibody-3B9C1-Western-Blot-NBP2-61823-img0003.jpg "Western Blot: BCL9-2 Antibody (3B9C1) [NBP2-61823] - Analysis using BCL9L mAb against human BCL9L (AA: 606-751) recombinant protein. (Expected MW is 41.9 kDa)")
![Immunohistochemistry: BCL9-2 Antibody (3B9C1) [NBP2-61823] - Analysis of paraffin-embedded cervical cancer tissues using BCL9L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BCL9-2-Antibody-3B9C1-Immunohistochemistry-NBP2-61823-img0002.jpg)
![Western Blot: BCL9-2 Antibody (3B9C1) [NBP2-61823] - Analysis using BCL9L mAb against HEK293 (1) and BCL9L (AA: 606-751)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BCL9-2-Antibody-3B9C1-Western-Blot-NBP2-61823-img0004.jpg)
![ELISA: BCL9-2 Antibody (3B9C1) [NBP2-61823] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BCL9-2-Antibody-3B9C1-ELISA-NBP2-61823-img0001.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NB100-665-img0001.jpg "Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]")
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0004.jpg "Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in cynomolgus heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0001.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in human colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0002.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0003.jpg)
![Western Blot: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32144) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-27820241631646.jpg "Western Blot: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32144) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32144) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-29820241492678.jpg)
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-2920241192361.jpg)
![Western Blot: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-278202415242648.jpg "Western Blot: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32143) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-298202414333636.jpg)
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-29202410334015.jpg)
![Western Blot: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Western blot analysis of TIMP-1 on different lysates with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW1990 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-288202412493478.jpg "Western Blot: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Western blot analysis of TIMP-1 on different lysates with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW1990 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33022) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-298202414115428.jpg)
![Flow Cytometry: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Flow cytometric analysis of HT-29 cells labeling TIMP-1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33022, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-392024884693.jpg)