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![Western Blot: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiMXD4.(1:2000)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Western-Blot-NBP2-72567-img0001.jpg "Western Blot: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiMXD4.(1:2000)")
![Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human tonsil within the normal limits using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72567-img0004.jpg)
![Flow Cytometry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human keratinocytes, PFA-fixed, permeabilization: PBS-Triton 0.1% 20 minutes at RT, primary antibody at 1:250 in PBS-2% BSA, secondary antibody conjugated to Alexa Fluor 594. Red: MAD4 antibody, Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72567-img0002.jpg)
![Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human liver tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72567-img0003.jpg)
![Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Paraffin-embedded human Kidney tissue within the normal limits using antiMXD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72567-img0005.jpg)
![Immunohistochemistry: E2F-1 Antibody (1D12) [NBP3-26609] - Image of E2F-1 Antibody (1D12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-262202414471553.jpg "Immunohistochemistry: E2F-1 Antibody (1D12) [NBP3-26609] - Image of E2F-1 Antibody (1D12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: E2F-1 Antibody (1D12) [NBP3-26609] - Image of E2F-1 Antibody (1D12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-27220248471914.jpg)
![Western Blot: E2F-1 Antibody (1D12) [NBP3-26609] - Positive Western Blot detected in: Jurkat whole cell lysate, PC-3 whole cell lysate, Rat Heart tissue. All lanes: E2F-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47 kDa Observed band size: 60 kDa](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-2822024115259.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (1D12) [NBP3-26609] - Staining of Hela Cells with E2F-1 Antibody (1D12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-282202412493640.jpg)
![Flow Cytometry: E2F-1 Antibody (1D12) [NBP3-26609] - Overlay histogram showing Hela cells stained with E2F-1 Antibody (1D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-282202414312778.jpg)
![Western Blot: Cdk6 Antibody [NBP2-15846] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cdk6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0004.jpg "Western Blot: Cdk6 Antibody [NBP2-15846] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cdk6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Cdk6 Antibody [NBP2-15846] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0001.jpg)
![Western Blot: Cdk6 Antibody [NBP2-15846] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0002.jpg)
![CDK6 antibody [N3C3] detects CDK6 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK6 protein stained by CDK6 antibody [N3C3] (NBP2-15846) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15846_rabbit-polyclonal-cdk6-antibody-immunocytochemistry-immunofluorescence-2611202518395722.jpg)
![Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Western-Blot-NBP2-32263-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunohistochemistry-NBP2-32263-img0004.jpg)
![Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunohistochemistry-NBP2-32263-img0003.jpg)
![Immunoprecipitation: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by rabbit anti-NAT10 antibody BL15900. For blotting immunoprecipitated NAT10, NBP2-32263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunoprecipitation-NBP2-32263-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: NAT10 Antibody [NBP2-32263] - Detection of human NAT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by another rabbit anti-NAT10 antibody. For blotting immunoprecipitated NAT10, NBP2-32263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunoprecipitation-NBP2-32263-img0005.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989] - Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Antibody (SPM350).](http://images.novusbio.com/fullsize/PCNA-Antibody-SPM350-Immunohistochemistry-Paraffin-NBP2-32989-img0002.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989] - Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Antibody (SPM350).")
![Immunocytochemistry/Immunofluorescence: Geminin Antibody [NBP2-16639] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16639-img0002.jpg "Immunocytochemistry/Immunofluorescence: Geminin Antibody [NBP2-16639] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-16639] - Detects Geminin protein at nucleus on breast carcinoma.](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunohistochemistry-Paraffin-NBP2-16639-img0003.jpg)
![Immunoprecipitation: Geminin Antibody [NBP2-16639] - HCT-116 whole cell extracts using 5 ug of Geminin antibody. Western blot analysis was performed using Geminin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunoprecipitation-NBP2-16639-img0004.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-20356-img0004.jpg "Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: PTTG1 Antibody [NBP2-20287] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PTTG1-Antibody-Western-Blot-NBP2-20287-img0001.jpg "Western Blot: PTTG1 Antibody [NBP2-20287] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PTTG1 Antibody [NBP2-20287] - Securin antibody detects PTTG1 protein at on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. Securin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PTTG1-Antibody-Immunohistochemistry-Paraffin-NBP2-20287-img0004.jpg)
![Immunoprecipitation: PTTG1 Antibody [NBP2-20287] - Securin antibody immunoprecipitates Securin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of Securin protein by 2 ug of Securin antibody 12% SDS-PAGE The immunoprecipitated Securin protein was detected by Securin antibody diluted at 1:1000](http://images.novusbio.com/fullsize/PTTG1-Antibody-Immunoprecipitation-NBP2-20287-img0003.jpg)
![Immunohistochemistry-Paraffin: NUP160 Antibody [NBP2-23718] - Analysis of NUP160 in formalin-fixed, paraffin-embedded human brain tissue using preimmune sera (top) and this antibody (bottom) at 1:100.](http://images.novusbio.com/fullsize/NUP160-Antibody-Immunohistochemistry-Paraffin-NBP2-23718-img0001.jpg "Immunohistochemistry-Paraffin: NUP160 Antibody [NBP2-23718] - Analysis of NUP160 in formalin-fixed, paraffin-embedded human brain tissue using preimmune sera (top) and this antibody (bottom) at 1:100.")
![Western Blot: BNC1 Antibody [NBP2-24721] - Analysis of BNC1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, and 3) rat lysate using NBP2-24721 at 7 ug/ml and 4 ug/ml, respectively.](http://images.novusbio.com/fullsize/BNC1-Antibody-Western-Blot-NBP2-24721-img0001.jpg "Western Blot: BNC1 Antibody [NBP2-24721] - Analysis of BNC1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, and 3) rat lysate using NBP2-24721 at 7 ug/ml and 4 ug/ml, respectively.")
![Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Western-Blot-NBP2-34331-img0003.jpg "Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Analysis using the Azide and BSA Free version of NBP2-34331. Staining of human Tonsil stained with Cdk1 MAb (POH-1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Immunohistochemistry-Paraffin-NBP2-34331-img0001.jpg)