| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16891-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03232-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52519-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89862-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99548-50ul | |||
![Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Smad2-[p-Ser467]-Antibody-Western-Blot-NBP3-16891-img0001.jpg "Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Rat testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125711..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Mouse testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125830..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Human kidney tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125949..jpg)
![Western Blot: S100A11 Antibody [NBP3-03232] - Analysis of extracts of various cell lines, using S100A11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/S100A11-Antibody-Western-Blot-NBP3-03232-img0004.jpg "Western Blot: S100A11 Antibody [NBP3-03232] - Analysis of extracts of various cell lines, using S100A11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: S100A11 Antibody [NBP3-03232] - Analysis of U2OS cells using S100A11 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/S100A11-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03232-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: S100A11 Antibody [NBP3-03232] - Analysis of extracts from normal (control) and S100A11 knockout (KO) HeLa cells, using S100A11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/S100A11-Antibody-Knockout-Validated-NBP3-03232-img0003.jpg)
![Immunoprecipitation: S100A11 Antibody [NBP3-03232] - Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug S100A11 antibody (NBP3-03232). Western blot was performed from the immunoprecipitate using S100A11 antibody (NBP3-03232) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/S100A11-Antibody-Immunoprecipitation-NBP3-03232-img0005.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mouse mAb against Hela (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0005.jpg "Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mouse mAb against Hela (1), and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis of paraffin-embedded ovarian cancer tissues using NME2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Immunohistochemistry-Paraffin-NBP2-52519-img0004.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - nalysis using NME2 mAb against HEK293 (1) and NME2 (AA: FULL(1-152))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0003.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mAb against human NME2 (AA: FULL(1-152)) recombinant protein. (Expected MW is 43.2 kDa)](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0002.jpg)
![ELISA: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-ELISA-NBP2-52519-img0007.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Western-Blot-NBP2-89862-img0002.jpg "Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa")
![Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 2 uL anti-PRDX5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PRDX5 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Immunoprecipitation-NBP2-89862-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: PRDX5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Knockout-Validated-NBP2-89862-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89862 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89862_rabbit-monoclonal-peroxiredoxin-5-antibody-002-235202514492332.png)
![Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-278202415212410.jpg "Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32668) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-298202414333643.jpg)
![Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunocytochemistry analysis of Hela cells labeling Nucleolin with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-29202410334079.jpg)
![Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-27820241633125.png "Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32288) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-29820241435277.jpg)
![Immunocytochemistry/ Immunofluorescence: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunocytochemistry analysis of 293 cells labeling PA2G4/EBP1 with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-2920241192382.jpg)
![Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-278202415295570.jpg "Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-278202416285221.jpg "Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29820241420942.jpg)
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunocytochemistry analysis of HeLa cells treated with or without 50nM dexamethasone for 16 hours labeling p57 Kip2 with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29202410484891.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0005.jpg "Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0001.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0002.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0003.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0004.jpg)
![Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-288202412442950.jpg "Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue untreated / treated with lambda pp / phospho-peptide / non-phospho-peptide with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-298202414333624.jpg)
![Immunocytochemistry/ Immunofluorescence: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunocytochemistry analysis of Jurkat cells treated with or without UV for 1 hour labeling p38 alpha with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-2920241114829.jpg)