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![Western Blot: IL12B Antibody [NB100-93409] - staining of Mouse (A) and Rat (B) Skin lysate (35ug protein in RIPA buffer). Primary incubation was](http://images.novusbio.com/fullsize/IL12B-Antibody-Western-Blot-NB100-93409-img0004.jpg "Western Blot: IL12B Antibody [NB100-93409] - staining of Mouse (A) and Rat (B) Skin lysate (35ug protein in RIPA buffer). Primary incubation was")
![Western Blot: CIRBP Antibody [NBP1-52066] - NBP1-52066 (0.05ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CIRBP-Antibody-Western-Blot-NBP1-52066-img0002.jpg "Western Blot: CIRBP Antibody [NBP1-52066] - NBP1-52066 (0.05ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Reg1A Antibody [NBP1-98445] - Sample Tissue: Human Fetal Kidney Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Reg1A-Antibody-Western-Blot-NBP1-98445-img0003.jpg "Western Blot: Reg1A Antibody [NBP1-98445] - Sample Tissue: Human Fetal Kidney Antibody Dilution: 1.0 ug/ml")
![Western Blot: Reg1A Antibody [NBP1-98445] - Mouse Pancreas lysate, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Reg1A-Antibody-Western-Blot-NBP1-98445-img0002.jpg)
![Western Blot: Granulin Antibody (4R8W1) [NBP3-33412] - Western blot analysis of lysates from Mouse liver, using Granulin Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33412_rabbit-granulin-mab-4r8w1-912202415175269.jpg "Western Blot: Granulin Antibody (4R8W1) [NBP3-33412] - Western blot analysis of lysates from Mouse liver, using Granulin Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: Granulin Antibody (4R8W1) [NBP3-33412] - Western blot analysis of lysates from HeLa cells, using Granulin Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33412_rabbit-granulin-mab-4r8w1-91220241514362.jpg)
![Immunohistochemistry: Abeta 42 Antibody (PSH02-83) [NBP3-32078] - Immunofluorescence analysis of paraffin-embedded AD mouse brain tissue labeling Abeta 42 with Rabbit anti-Abeta 42 antibody (NBP3-32078) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32078, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32078_rabbit-abeta-42-mab-psh02-83-298202414161849.jpg "Immunohistochemistry: Abeta 42 Antibody (PSH02-83) [NBP3-32078] - Immunofluorescence analysis of paraffin-embedded AD mouse brain tissue labeling Abeta 42 with Rabbit anti-Abeta 42 antibody (NBP3-32078) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32078, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Abeta 42 Antibody (PSH02-83) [NBP3-32078] - Immunohistochemical analysis of paraffin-embedded mouse model of Alzheimer's brain tissue with Rabbit anti-Abeta 42 antibody (NBP3-32078) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32078) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32078_rabbit-abeta-42-mab-psh02-83-298202413585551.jpg)
![Western Blot: GFI-1 Antibody [NBP2-82227] - WB Suggested Anti-GFI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/GFI-1-Antibody-Western-Blot-NBP2-82227-img0001.jpg "Western Blot: GFI-1 Antibody [NBP2-82227] - WB Suggested Anti-GFI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate")
![Western Blot: GFI-1 Antibody [NBP2-82227] - Host: Mouse. Target Name: GFI1. Sample Tissue: Mouse Liver. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/GFI-1-Antibody-Western-Blot-NBP2-82227-img0002.jpg)
![Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23232_rabbit-p38-alpha-pab-199202312403713.jpg "Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SESN1 antibody [HL1750] (NBP3-48662) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-48662_rabbit-sesn1-mab-hl1750-western-blot-141120251826019.jpg "<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SESN1 antibody [HL1750] (NBP3-48662) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")