| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04622-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35368-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99593-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99003-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08505 | |||
![Western Blot: FHL3 Antibody [NBP3-04622] - Analysis of extracts of various cell lines, using FHL3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/FHL3-Antibody-Western-Blot-NBP3-04622-img0001.jpg "Western Blot: FHL3 Antibody [NBP3-04622] - Analysis of extracts of various cell lines, using FHL3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/ Immunofluorescence: FHL3 Antibody - BSA Free [NBP3-04622] - Immunofluorescence analysis of HeLa cells using FHL3 Rabbit pAb (A8679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04622_rabbit-polyclonal-fhl3-antibody-52202519313445.jpg)
![Western Blot: CCAR1 Antibody [NBP3-35368] - Western blot analysis of various lysates using CCAR1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35368_rabbit-ccar1-pab-912202415133430.jpg "Western Blot: CCAR1 Antibody [NBP3-35368] - Western blot analysis of various lysates using CCAR1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunoprecipitation: CCAR1 Antibody [NBP3-35368] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug CCAR1 antibody. Western blot was performed from the immunoprecipitate using CCAR1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-35368_rabbit-ccar1-pab-9122024141819.jpg)
![Western Blot: IFRD1 Antibody [NBP2-99593] - Anti-IFRD1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP2-99593-img0003.jpg "Western Blot: IFRD1 Antibody [NBP2-99593] - Anti-IFRD1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa")
![Immunocytochemistry/Immunofluorescence: IFRD1 Antibody [NBP2-99593] - Immunofluorescence staining of IFRD1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99593 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .](http://images.novusbio.com/fullsize/nbp2-99593_rabbit-polyclonal-ifrd1-antibody-235202521525033.png)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP2-99003-img0003.jpg "Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in cynomolgus heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP2-99003-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP2-99003-img0002.jpg)
![Western Blot: MyoD Antibody (JE60-47) [NBP3-32630] - Western blot analysis of MyoD on different lysates with Rabbit anti-MyoD antibody (NBP3-32630) at 1/1,000 dilution. Lane 1: RD cell lysate Lane 2: HEK-293 cell lysate (negative) Lane 3: HeLa cell lysate (negative) Lane 4: RD cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 35 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32630) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32630_rabbit-myod-mab-je60-47-278202416285250.jpg "Western Blot: MyoD Antibody (JE60-47) [NBP3-32630] - Western blot analysis of MyoD on different lysates with Rabbit anti-MyoD antibody (NBP3-32630) at 1/1,000 dilution. Lane 1: RD cell lysate Lane 2: HEK-293 cell lysate (negative) Lane 3: HeLa cell lysate (negative) Lane 4: RD cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 35 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32630) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (JE60-47) [NBP3-32630] - Immunocytochemistry analysis of RD (positive) and HEK-293 (negative) labeling MyoD with Rabbit anti-MyoD antibody (NBP3-32630) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MyoD antibody (NBP3-32630) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32630_rabbit-myod-mab-je60-47-2920241192395.jpg)
![Western Blot: CCAR1 Antibody [NB500-188] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-188 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-188-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: CCAR1 Antibody [NB500-188] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-188 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CCAR1 Antibody [NB500-188] - on HeLa whole cell lysate using NB500-188. CCAR1 was also immunoprecipitated with another rabbit anti-CCAR1 antibody, NB500-187, and NB500-188. Immunoprecipitated CCAR1 was blotted using NB500-188.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-188-img0001.jpg)
![Western Blot: MyoD Antibody (RM369) [NBP2-77430] - Western Blot of mouse skeletal muscle tissue lysate using RM369 at a 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp2-77430_rabbit-monoclonal-myod-antibody-rm369-24620241332459.png "Western Blot: MyoD Antibody (RM369) [NBP2-77430] - Western Blot of mouse skeletal muscle tissue lysate using RM369 at a 1:2000 dilution.")
![Immunohistochemistry-Paraffin: MyoD Antibody (RM369) [NBP2-77430] - Mouse skeletal muscle tissue lysate using anti-MyoD1 rabbit monoclonal antibody (Clone RM369) at a 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp2-77430_rabbit-monoclonal-myod-antibody-rm369-immunohistochemistry-paraffin-189202523374021.png)
![SDS-Page: Recombinant Human SPAG9 Protein [H00009043-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-SPAG9-Protein-SDS-Page-H00009043-P01-img0002.jpg "SDS-Page: Recombinant Human SPAG9 Protein [H00009043-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: MyoD Antibody (rMYD712) [NBP3-07394] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Mouse Recombinant Monoclonal Antibody (rMYD712).](http://images.novusbio.com/fullsize/MyoD-Antibody-rMYD712-Immunohistochemistry-Paraffin-NBP3-07394-img0002.jpg "Immunohistochemistry-Paraffin: MyoD Antibody (rMYD712) [NBP3-07394] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Mouse Recombinant Monoclonal Antibody (rMYD712).")
![Protein Array: MyoD Antibody (rMYD712) [NBP3-07394] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MyoD Mouse Recombinant Monoclonal Antibody (rMYD712).](http://images.novusbio.com/fullsize/MyoD-Antibody-rMYD712-Protein-Array-NBP3-07394-img0001.jpg)
![Western Blot: CCAR1 Antibody [NBP1-46158] - NBP1-46158 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NBP1-46158-img0002.jpg "Western Blot: CCAR1 Antibody [NBP1-46158] - NBP1-46158 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: SPAG9 Antibody - BSA Free [NBP3-45853] - RAW 264.7 cells were subjected to SDS PAGE followed by western blot with ( SPAG9 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-45853_rabbit-spag9-pab-bsa-free-2842025138781.jpg "Western Blot: SPAG9 Antibody - BSA Free [NBP3-45853] - RAW 264.7 cells were subjected to SDS PAGE followed by western blot with ( SPAG9 Antibody) at dilution of 1:1000")