| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48002-0.1mg | |||
| IHC-Prediluted | NBP2-48186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48318 | |||
| Azide and BSA Free | NBP3-11498 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97494-0.2mg | |||
| BSA Free | NBP1-97494-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80036-50ug | |||
![Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.](http://images.novusbio.com/fullsize/HSP27-Antibody-Western-Blot-NB120-5579-img0003.jpg "Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0008.jpg)
![Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754]- Western blot analysis of extracts from normal (control) and Lamin A + C knockout (KO) HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Knockout-Validated-NBP3-15754-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754]- Western blot analysis of extracts from normal (control) and Lamin A + C knockout (KO) HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of U2OS cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunocytochemistry-Immunofluorescence-NBP3-15754-img0007.jpg)
![Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A + C antibody (NBP3-15754). Western blot was performed from the immunoprecipitate using Lamin A + C antibody (NBP3-15754) at a dilition of 1:20000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunoprecipitation-NBP3-15754-img0011.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517311038.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517333869.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517315547.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Confocal imaging of U-2 OS cells using [KO Validated] Lamin A + C Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517315568.jpg)
![Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunoprecipitation analysis of 300 ug extracts from PC-12 cells using 3 ug [KO Validated] Lamin A + C Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:100000.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517352763.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517311069.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - The STORM super-resolution (SR) imaging of U-2 OS cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:200 with 3% paraformaldehyde (PFA) +0.1% glutaraldehyde (GA) fixation. The immunostaining was performed by Full Automatic Immunofluorescence Workflow System (Workflow Ultra300, Nano-Micro imaging, China). Image was performed with Single-Molecule Localization Super-Resolution Microscopy (STORM Ultra300, Nano-Micro imaging, China). We acknowledge Nano-Micro imaging Biotechnology Co., Ltd. in SR image processing and kindly providing this image.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517344285.jpg)
![Western Blot: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Western blot analysis of lysates from A549 cells using [KO Validated] Lamin A + C Rabbit mAb at 1:240000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517315542.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunofluorescence analysis of L929 cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-5220251733550.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunofluorescence analysis of H9C2 cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-5220251734531.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517352736.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Confocal immunofluorescence analysis of HeLa cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-5220251733511.jpg)
![Western Blot: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Western blot analysis of various lysates using [KO Validated] Lamin A + C Rabbit mAb at 1:300000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517344232.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15754_rabbit-lamin-a-c-mab-4l8q0-52202517361239.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19323-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human breast carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0012.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Lamin A/C antibody detects LMNA protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lamin A/C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse prostate. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse fore brain. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0008.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse skin. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human endometrial carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0011.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0016.jpg "Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Immunocytochemistry-Immunofluorescence-H00002965-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Immunohistochemistry-Paraffin-H00002965-M01-img0011.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 over-expressed 293 cell line, cotransfected with GTF2H1 Validated Chimera RNAi ( Cat # H00002965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (Cat # H00002965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0012.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Analysis of GTF2H1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0014.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Analysis of GTF2H1 expression in Jurkat.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0015.jpg)
![Sandwich ELISA: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Detection limit for recombinant GST tagged GTF2H1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Sandwich-ELISA-H00002965-M01-img0013.jpg)
![Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-24533-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1:5000 and 2) peptide affinity purified HSP27 antibody at 2.0 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP2-24533-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-Paraffin-NBP2-24533-img0002.jpg)
![Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Western-Blot-NBP2-44975-img0006.jpg "Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (HSPB1/774) [NBP2-44975] - ICC/IF analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunofluorescence-NBP2-44975-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0002.jpg)
![Flow Cytometry: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Flow-Cytometry-NBP2-44975-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0006.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) [NBP2-44611] - Human Tonsil stained with Nucleolin Monoclonal Antibody (SPM614).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunohistochemistry-Paraffin-NBP2-44611-img0001.jpg)
![Flow Cytometry: Nucleolin Antibody (SPM614) [NBP2-44611] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (SPM614).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Flow-Cytometry-NBP2-44611-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Antibody (SPM614)(Green) Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0005.jpg)
![Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Western-Blot-NBP2-34621-img0002.jpg "Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34621-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34621-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0005.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.](http://images.novusbio.com/fullsize/Crystallin-AB-Antibody-1B6-1-3G4-Western-Blot-NBP1-97494-img0004.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.")
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Human Heart tissue stained with alpha B-Crystallin, mAb (1B6.1-3G4) at 10ug/ml.](http://images.novusbio.com/fullsize/Crystallin-AB-Antibody-1B6-1-3G4-Immunohistochemistry-Paraffin-NBP1-97494-img0003.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1:2000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Western-Blot-NBP2-80036-img0006.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1:2000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunofluorescent confocal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt (Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunocytochemistry-Immunofluorescence-NBP2-80036-img0004.jpg)
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) in human kidney tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed and paraffin-embedded (FFPE) human kidney showing cytoplasmic staining (200x, brown color).](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunohistochemistry-Paraffin-NBP2-80036-img0002.jpg)
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF), mAb (OMNI379) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (15 ug/mL).](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunohistochemistry-Paraffin-NBP2-80036-img0001.jpg)
![PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1:2000 dilution. 1. Human Nampt (Visfatin/PBEF) (His-tagged). 2. Human Nampt (Visfatin/PBEF) (FLAG(R)-tagged). 3. Mouse Nampt (Visfatin/PBEF) (His-tagged). 4. Rat Nampt (Visfatin/PBEF) (His-tagged). 5. Human Nampt (Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-NBP2-80036-img0005.jpg)
![Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted and visualized by western blot with anti-human Nampt (Visfatin/PBEF) pAb.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunoprecipitation-NBP2-80036-img0007.jpg)