| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22415-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06496-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98336-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99017-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27149-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20107-0.025mg | |||
![Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Western-Blot-NBP1-22410-img0005.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Immunocytochemistry-Immunofluorescence-NBP1-22410-img0006.jpg)
![Western Blot: Dicer Antibody (S167-7) [NBP2-22415] - Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Western-Blot-NBP2-22415-img0004.jpg "Western Blot: Dicer Antibody (S167-7) [NBP2-22415] - Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.")
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Dicer Antibody (D) Composite.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Immunocytochemistry-Immunofluorescence-NBP2-22415-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Dicer Antibody (D) Composite.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Immunocytochemistry-Immunofluorescence-NBP2-22415-img0003.jpg)
![Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Western-Blot-NBP3-06496-img0001.jpg "Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa")
![Immunohistochemistry-Paraffin: CEND1 Antibody [NBP3-06496] - Staining of human CEND1 in human brain with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunohistochemistry-Paraffin-NBP3-06496-img0002.jpg)
![Immunoprecipitation: CEND1 Antibody [NBP3-06496] - Lane A:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-CEND1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CEND1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunoprecipitation-NBP3-06496-img0003.jpg)
![Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98336-img0004.jpg "Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-98336] - Immunochemical staining of mouse E-Cadherin in mouse stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-98336-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP2-98336] - Mouse E-Cadherin was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-Mouse E-Cadherin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse E-Cadherin rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band size: 130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP2-98336-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: Raji cell lysate Lane 7: HeLa cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 44 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-278202416331292.jpg "Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: Raji cell lysate Lane 7: HeLa cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 44 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32285) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-298202414304159.jpg)
![Flow Cytometry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Flow cytometric analysis of K-562 cells labeling E2F-4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32285, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-29202416372181.jpg)
![Western Blot: PRC1 Antibody [NBP2-99017] - Anti-PRC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: MDA-MB231 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 68 kDa](http://images.novusbio.com/fullsize/PRC1-Antibody-Western-Blot-NBP2-99017-img0002.jpg "Western Blot: PRC1 Antibody [NBP2-99017] - Anti-PRC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: MDA-MB231 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 68 kDa")
![Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99017] - Immunochemical staining of human PRC1 in human testis with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRC1-Antibody-Immunohistochemistry-Paraffin-NBP2-99017-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-99017] - Immunofluorescence staining of PRC1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99017 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-99017_rabbit-polyclonal-prc1-antibody-235202511444419.png)
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-10420249141932.jpg%3Fv=1666704272 "Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: HBO1 Antibody (4E12H12) [NBP3-27149] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024915839.jpg%3Fv=1666704272)
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against human HBO1 (AA: 1-200) recombinant protein. (Expected MW is 48.1 kDa)](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024915849.jpg%3Fv=1666704272)
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mouse mAb against MOLT4 (1), COS7 (2), F9 (3), HepG2 (4), PC-2 (5), U251 (6), MCF-7 (7), and NIH/3T3 (8) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024920235.jpg%3Fv=1666704272)
![Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924254.png "Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (NBP3-20107, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023964610.png)
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41A-1 (NBP3-20107) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20107, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924231.png)
![Western Blot: HBO1 Antibody [NBP2-16784] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/HBO1-Antibody-Western-Blot-NBP2-16784-img0002.jpg "Western Blot: HBO1 Antibody [NBP2-16784] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: HBO1 Antibody [NBP2-16784] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HBO1-Antibody-Immunohistochemistry-Paraffin-NBP2-16784-img0003.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-158] - Samples : Whole cell lysate (25 ug) from BT549 (positive control), U2OS (negative control) and p14ARF/p16INK4a transfected IMR90 cells. Antibody : Affinity purified rabbit anti-p14ARF/p16INK4a used at 1 ug/ml. Detection : Chemiluminescence.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-158-img0002.jpg "Western Blot: p14ARF/CDKN2A Antibody [NB200-158] - Samples : Whole cell lysate (25 ug) from BT549 (positive control), U2OS (negative control) and p14ARF/p16INK4a transfected IMR90 cells. Antibody : Affinity purified rabbit anti-p14ARF/p16INK4a used at 1 ug/ml. Detection : Chemiluminescence.")
![Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-158] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF/p16INK4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-NB200-158-img0003.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-159-img0002.jpg "Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.")
![Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-159] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF/p16INK4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-NB200-159-img0003.jpg)
![Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody [NB200-159] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-p14ARF used at a dilution of 1:250.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-Paraffin-NB200-159-img0001.jpg)