| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26608-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26236-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33196 | |||
| IHC-Prediluted | NBP2-44558 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26261-50ul | |||
![Western Blot: DNAJA2 Antibody [NBP2-16190] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-Western-Blot-NBP2-16190-img0001.jpg "Western Blot: DNAJA2 Antibody [NBP2-16190] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry: DNAJA2 Antibody [NBP2-16190] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using DNAJA2 antibody [N1C2] (NBP2-16190) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-16190_rabbit-polyclonal-dnaja2-antibody-91020242075928.jpg)
![Western Blot: DNAJA2 Antibody [NBP2-16190] - Sample (30 ug of whole cell lysate) A: whole zebrafish B: zebrafish brain 10% SDS PAGE NBP2-16190 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16190_rabbit-polyclonal-dnaja2-antibody-91020242091498.jpg)
![Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-262202414471533.jpg "Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: E2F-4 Antibody (24E12) [NBP3-26608] - Overlay Peak curve showing MCF7 cells stained with E2F-4 Antibody (24E12) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-282202414312787.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody (24E12) [NBP3-26608] -Immunofluorescence staining of Hela cell with E2F-4 Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 513-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-29120251248506.jpg)
![Western Blot: PCAF Antibody [NBP1-31192] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug A375 whole cell lysate/extract 7.5 % SDS-PAGE PCAF antibody [C3], C-term dilution: 1:1000](http://images.novusbio.com/fullsize/PCAF-Antibody-Western-Blot-NBP1-31192-img0005.jpg "Western Blot: PCAF Antibody [NBP1-31192] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug A375 whole cell lysate/extract 7.5 % SDS-PAGE PCAF antibody [C3], C-term dilution: 1:1000")
![Western Blot: PCAF Antibody [NBP1-31192] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PCAF-Antibody-Western-Blot-NBP1-31192-img0003.jpg)
![Western Blot: PCAF Antibody [NBP1-31192] - HeLa whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term diluted at 1:1000.](http://images.novusbio.com/fullsize/PCAF-Antibody-Western-Blot-NBP1-31192-img0004.jpg)
![Western Blot: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 on HeLa whole cell lysate using NB100-2364. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody NB100-2363.](http://images.novusbio.com/fullsize/ZWINT-Antibody-Western-Blot-NB100-2364-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 on HeLa whole cell lysate using NB100-2364. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody NB100-2363.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: ZWINT Antibody [NB100-2364] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-ZWINT-1 used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/ZWINT-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2364-img0002.jpg)
![Immunohistochemistry: NEK4 Antibody [NBP1-42686] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunohistochemistry-NBP1-42686-img0003.jpg "Immunohistochemistry: NEK4 Antibody [NBP1-42686] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: NEK4 Antibody [NBP1-42686] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody used for IP at 3 mcg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42687, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunoprecipitation-NBP1-42686-img0002.jpg)
![Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-26220241322159.jpg "Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).")
![Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] - Image of HDAC6 Antibody (2H12) diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-262202414471573.jpg)
![Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate. All lanes: HDAC6 Antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 132, 115 KDa Observed band size: 160 KDa](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-282202411413619.jpg)
![Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (CD30/412) [NBP2-29425] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma (20X) stained with CD30 Ab (CD30/412).](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-CD30-412-Immunohistochemistry-Paraffin-NBP2-29425-img0006.jpg "Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (CD30/412) [NBP2-29425] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma (20X) stained with CD30 Ab (CD30/412).")
![Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (CD30/412) [NBP2-29425] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma(20X) stained with CD30 Ab (CD30/412).](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-CD30-412-Immunohistochemistry-Paraffin-NBP2-29425-img0005.jpg)
![Immunohistochemistry-Paraffin: NOLC1 Antibody [NBP1-22981] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NOPP140 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NOLC1-Antibody-Immunohistochemistry-Paraffin-NBP1-22981-img0005.jpg "Immunohistochemistry-Paraffin: NOLC1 Antibody [NBP1-22981] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NOPP140 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: NOLC1 Antibody [NBP1-22981] - Detection of human NOPP140 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from K-562 cells. Antibodies: Affinity purified rabbit anti-NOPP140 antibody NBP1-22981 used for IP at 3 ug/mg lysate. NOPP140 was also immunoprecipitated by rabbit anti-NOPP140 antibody NBP1-22982, which recognizes a downstream epitope. For blotting immunoprecipitated NOPP140, NBP1-22981 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/NOLC1-Antibody-Immunoprecipitation-NBP1-22981-img0006.jpg)
![Immunohistochemistry: Hexim 1 Antibody [NBP1-71866] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71866-img0005.jpg "Immunohistochemistry: Hexim 1 Antibody [NBP1-71866] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).")
![Immunoprecipitation: Hexim 1 Antibody [NBP1-71866] - Whole cell lysate (1 mg for IP, used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71867 and NBP1-71868, which recognize downstream epitopes.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunoprecipitation-NBP1-71866-img0002.jpg)
![Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB100-230-img0089.jpg "Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (50, 15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB100-230-img0088.jpg)
![Immunohistochemistry-Paraffin: BMP-10 Antibody (A1) [NBP3-42003] - DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 30ug/ml NBP3-42003](http://images.novusbio.com/fullsize/nbp3-42003_mouse-bmp-10-mab-a1-612202421395360.jpg "Immunohistochemistry-Paraffin: BMP-10 Antibody (A1) [NBP3-42003] - DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 30ug/ml NBP3-42003")
![Immunohistochemistry-Paraffin: BMP-10 Antibody (A1) [NBP3-42003] - DAB staining on IHC-P; Samples: Human Liver Tissue.](http://images.novusbio.com/fullsize/nbp3-42003_mouse-bmp-10-mab-a1-612202421373073.jpg)
![Western Blot: BMP-10 Antibody (A1) [NBP3-42003] - Sample: Recombinant BMP-10, Human.](http://images.novusbio.com/fullsize/nbp3-42003_mouse-bmp-10-mab-a1-612202421283848.jpg)
![Western Blot: USP22 Antibody (18H6) [NBP3-26261] - Positive Western Blot detected in: A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate. All lanes: USP22 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 59 kDa Observed band size: 55-72 kDa](http://images.novusbio.com/fullsize/nbp3-26261_rabbit-usp22-mab-18h6-282202411472117.jpg "Western Blot: USP22 Antibody (18H6) [NBP3-26261] - Positive Western Blot detected in: A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate. All lanes: USP22 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 59 kDa Observed band size: 55-72 kDa")
![Immunocytochemistry/Immunofluorescence: USP22 Antibody (18H6) [NBP3-26261] - Staining of Hela cell with USP22 Antibody (18H6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 507-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26261_rabbit-usp22-mab-18h6-282202412475133.jpg)
![Flow Cytometry: USP22 Antibody (18H6) [NBP3-26261] - Overlay Peak curve showing Hela cells stained with USP22 Antibody (18H6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26261_rabbit-usp22-mab-18h6-282202414312718.jpg)