| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-44986 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99422-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98936-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26671-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26260-50ul | |||
![Western Blot: CDC37 Antibody (6X0A3) [NBP3-16442] - Western blot analysis of extracts of various cell lines, using CDC37 Rabbit mAb (NBP3-16442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CDC37-Antibody-6X0A3-Western-Blot-NBP3-16442-img0001.jpg "Western Blot: CDC37 Antibody (6X0A3) [NBP3-16442] - Western blot analysis of extracts of various cell lines, using CDC37 Rabbit mAb (NBP3-16442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: SESN3 Antibody [NBP1-44985] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 53 KDa.](http://images.novusbio.com/fullsize/SESN3-Antibody-Western-Blot-NBP1-44985-img0001.jpg "Western Blot: SESN3 Antibody [NBP1-44985] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 53 KDa.")
![Western Blot: CDC37 Antibody [NBP2-99422] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-99422-img0004.jpg "Western Blot: CDC37 Antibody [NBP2-99422] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 50 kDa")
![Immunoprecipitation: CDC37 Antibody [NBP2-99422] - CDC37 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg A431 Whole Cell Lysate4 ul anti-CDC37 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CDC37 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-99422-img0003.jpg)
![Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32813_rabbit-tsc2-p-thr1462-mab-je43-81-288202412461624.jpg "Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33073_mouse-uba3-mab-1b6-6-5-r-28820241419156.jpg "Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: CDC37 Antibody [NBP2-98936] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-98936-img0002.jpg "Western Blot: CDC37 Antibody [NBP2-98936] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 50 kDa")
![Immunoprecipitation: CDC37 Antibody [NBP2-98936] - CDC37 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate4 ul anti-CDC37 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CDC37 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-98936-img0001.jpg)
![Western Blot: CDC37 Antibody [NBP1-70346] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-CDC37 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CDC37 was also immunoprecipitated by another rabbit anti-CDC37 antibody , which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP1-70346-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CDC37 Antibody [NBP1-70346] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-CDC37 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CDC37 was also immunoprecipitated by another rabbit anti-CDC37 antibody , which recognizes an upstream epitope.")
![Western Blot: CDC37 Antibody (10C3) [NBP3-26671] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate. All lanes: CDC37 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp3-26671_rabbit-cdc37-mab-10c3-28220241152525.jpg "Western Blot: CDC37 Antibody (10C3) [NBP3-26671] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate. All lanes: CDC37 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa Observed band size: 50 kDa")
![Flow Cytometry: CDC37 Antibody (10C3) [NBP3-26671] - Overlay histogram showing Hela cells stained with CDC37 Antibody (10C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26671_rabbit-cdc37-mab-10c3-282202414312749.jpg)
![Western Blot: 14-3-3 zeta Antibody (C3) [NBP3-41868] - Sample: Lane1: Rat Cerebrum lysate; Lane2: Raji cell lysate; Lane3: 3T3-L1 cell lysate; Lane4: PC3 cell lysate; Lane5: Jurkat cell lysate Primary Ab: 0.2ug/ml NBP3-41868; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41868_mouse-14-3-3-zeta-mab-c3-612202421262972.jpg "Western Blot: 14-3-3 zeta Antibody (C3) [NBP3-41868] - Sample: Lane1: Rat Cerebrum lysate; Lane2: Raji cell lysate; Lane3: 3T3-L1 cell lysate; Lane4: PC3 cell lysate; Lane5: Jurkat cell lysate Primary Ab: 0.2ug/ml NBP3-41868; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunocytochemistry/Immunofluorescence: USP22 Antibody (18E3) [NBP3-26260] - Staining of MCF-7 with USP22 Antibody (18E3) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 493-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26260_rabbit-usp22-mab-18e3-282202412475145.jpg "Immunocytochemistry/Immunofluorescence: USP22 Antibody (18E3) [NBP3-26260] - Staining of MCF-7 with USP22 Antibody (18E3) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 493-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: USP22 Antibody (18E3) [NBP3-26260] - Overlay Peak curve showing Hela cells stained with USP22 Antibody (18E3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26260_rabbit-usp22-mab-18e3-282202414183656.jpg)