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![Western Blot: USP16 Antibody [NBP3-38203] - Western blot analysis of various lysates, using USP16 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38203_rabbit-usp16-pab-912202415111853.jpg "Western Blot: USP16 Antibody [NBP3-38203] - Western blot analysis of various lysates, using USP16 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunoprecipitation: USP16 Antibody [NB100-81639] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody used for IP at 6 ug per reaction. USP16 was also immunoprecipitated by rabbit anti-USP16 antibody. For blotting immunoprecipitated USP16 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunoprecipitation-NB100-81639-img0001.jpg "Immunoprecipitation: USP16 Antibody [NB100-81639] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody used for IP at 6 ug per reaction. USP16 was also immunoprecipitated by rabbit anti-USP16 antibody. For blotting immunoprecipitated USP16 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: RTF1 Antibody [NB600-277] - Detection of human RTF1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RTF1 antibody NB600-277 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RTF1 was successfully immunoprecipitated by rabbit anti-RTF1 antibodies NB600-278 and NB100-61052, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/RTF1-Antibody-Western-Blot-NB600-277-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RTF1 Antibody [NB600-277] - Detection of human RTF1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RTF1 antibody NB600-277 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RTF1 was successfully immunoprecipitated by rabbit anti-RTF1 antibodies NB600-278 and NB100-61052, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Western Blot: RTF1 Antibody [NBP3-35771] - Western blot analysis of various lysates using RTF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35771_rabbit-rtf1-pab-1012202411353072.jpg "Western Blot: RTF1 Antibody [NBP3-35771] - Western blot analysis of various lysates using RTF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBP2-09831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.](http://images.novusbio.com/fullsize/USP16-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09831-img0001.jpg "Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBP2-09831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.")
![Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBL1-17647] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.](http://images.novusbio.com/fullsize/USP16-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17647-img0002.jpg "Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBL1-17647] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.")
![Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBP2-09188] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.](http://images.novusbio.com/fullsize/USP16-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09188-img0001.jpg "Western Blot: USP16 Overexpression Lysate (Adult Normal) [NBP2-09188] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP16.")
![Western Blot: UEV2 Overexpression Lysate (Adult Normal) [NBL1-17548] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UEV2.](http://images.novusbio.com/fullsize/UEV2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17548-img0002.jpg "Western Blot: UEV2 Overexpression Lysate (Adult Normal) [NBL1-17548] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UEV2.")
![SDS-Page: Recombinant Human UEV2 Protein [NBP1-45295] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-UEV2-Protein-SDS-Page-NBP1-45295-img0002.jpg "SDS-Page: Recombinant Human UEV2 Protein [NBP1-45295] - 15% SDS-PAGE (3ug)")