| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92653-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92848-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38567-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92872-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26200-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26236-50ul | |||
![Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts of various cell lines, using CETN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Centrin-2-Antibody-Western-Blot-NBP2-92653-img0003.jpg "Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts of various cell lines, using CETN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts from normal (control) and CETN2 knockout (KO) HeLa cells, using CETN2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Centrin-2-Antibody-Knockout-Validated-NBP2-92653-img0005.jpg)
![Western Blot: Cullin 3 Antibody [NBP2-92848] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Cullin 3 Antibody-Western Blot-NBP2-92848-img0002.jpg "Western Blot: Cullin 3 Antibody [NBP2-92848] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Cullin 3 Antibody [NBP2-92848] - Analysis of HeLa cells using Cullin 3 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92848-img0001.jpg)
![Western Blot: BCCIP Antibody [NBP3-38567] - Western blot analysis of various lysates using BCCIP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38567_rabbit-bccip-pab-912202415171153.jpg "Western Blot: BCCIP Antibody [NBP3-38567] - Western blot analysis of various lysates using BCCIP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunohistochemistry: BCCIP Antibody [NBP3-38567] - Immunohistochemistry analysis of paraffin-embedded Human breast using BCCIP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38567_rabbit-bccip-pab-912202415175244.jpg)
![Western Blot: Cullin 3 Antibody [NBP2-92872] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NBP2-92872-img0002.jpg "Western Blot: Cullin 3 Antibody [NBP2-92872] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Cullin 3 Antibody [NBP2-92872] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NBP2-92872-img0001.jpg)
![Western Blot: HDAC7 Antibody [NB100-2146] - Cell line lysates were separated on SDS-PAGE and probed with 1:500 Rabbit Anti-Histone Deacetylase 7 (HDAC7) (KG-17). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase ) and a chemiluminescent substrate.Lanes:1. HeLa nuclear2. NIH-3T3](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NB100-2146-img0002.jpg "Western Blot: HDAC7 Antibody [NB100-2146] - Cell line lysates were separated on SDS-PAGE and probed with 1:500 Rabbit Anti-Histone Deacetylase 7 (HDAC7) (KG-17). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase ) and a chemiluminescent substrate.Lanes:1. HeLa nuclear2. NIH-3T3")
![Immunoprecipitation: HDAC7 Antibody [NB100-2146] - Immunoprecipitation HDAC7 was immunoprecipitated from whole cell extract of human HEK 293T cells expressing HDAC7-FLAG using Rabbit Anti-HDAC7 (KG-17) antibody (NB100-2146). The IP samples were separated on SDS-PAGE and blotted with Rabbit Anti-FLAG antibody. The antibody was developed with Goat anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate. Lane 1: 1.8 ug Antibody immunoprecipitates HDAC7 from a RIPA extract of 180 ug of 293T cells expressing HDAC7-FLAG. Lane 2: negative control (extract without antibody).](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunoprecipitation-NB100-2146-img0001.jpg)
![Western Blot: Cullin 3 Antibody (JE37-29) [NBP3-32232] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32232) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: SW620 cell lysate Lane 3: HT-29 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 89 kDa Observed band size: 75 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32232) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32232_rabbit-cullin-3-mab-je37-29-27820241628528.jpg "Western Blot: Cullin 3 Antibody (JE37-29) [NBP3-32232] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32232) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: SW620 cell lysate Lane 3: HT-29 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 89 kDa Observed band size: 75 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32232) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cullin 3 Antibody (JE37-29) [NBP3-32232] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cullin 3 antibody (NBP3-32232) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32232) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32232_rabbit-cullin-3-mab-je37-29-298202414115436.jpg)
![Western Blot: Cullin 3 Antibody (JE57-80) [NBP3-32233] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32233) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: PC-12 cell lysate (20 ug/Lane) Lane 4: Mouse spleen tissue lysate (40 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Lane 7: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32233) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32233_rabbit-cullin-3-mab-je57-80-278202415212437.jpg "Western Blot: Cullin 3 Antibody (JE57-80) [NBP3-32233] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32233) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: PC-12 cell lysate (20 ug/Lane) Lane 4: Mouse spleen tissue lysate (40 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Lane 7: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32233) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cullin 3 Antibody (JE57-80) [NBP3-32233] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Cullin 3 antibody (NBP3-32233) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32233) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32233_rabbit-cullin-3-mab-je57-80-29820241492664.jpg)
![Immunohistochemistry: ILF3 Antibody (7H8) [NBP3-26200] - Image of ILF3 Antibody (7H8) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26200_rabbit-ilf3-mab-7h8-26220241522420.jpg "Immunohistochemistry: ILF3 Antibody (7H8) [NBP3-26200] - Image of ILF3 Antibody (7H8) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ILF3 Antibody (7H8) [NBP3-26200] - Image of ILF3 Antibody (7H8) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26200_rabbit-ilf3-mab-7h8-2722024847197.jpg)
![Immunocytochemistry/Immunofluorescence: ILF3 Antibody (7H8) [NBP3-26200] - Staining of Hela Cells with ILF3 Antibody (7H8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26200_rabbit-ilf3-mab-7h8-282202412493614.jpg)
![Western Blot: DIAPH-2 Antibody [NB600-280] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Diaphanous 2 antibody.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NB600-280-img0004.jpg "Western Blot: DIAPH-2 Antibody [NB600-280] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Diaphanous 2 antibody.")
![Western Blot: DIAPH-2 Antibody [NB600-280] - Whole cell lysate (50 ug) from HEK 293T cells. Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NB600-280-img0002.jpg)
![Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-26220241322159.jpg "Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).")
![Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] - Image of HDAC6 Antibody (2H12) diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-262202414471573.jpg)
![Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate. All lanes: HDAC6 Antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 132, 115 KDa Observed band size: 160 KDa](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-282202411413619.jpg)
![Immunohistochemistry: TOB2 Antibody - BSA Free [NBP3-45166] - Immunohistochemistry of paraffin-embedded human liver injury tissue slide using ( TOB2 Antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-45166_rabbit-tob2-pab-bsa-free-284202511333234.jpg "Immunohistochemistry: TOB2 Antibody - BSA Free [NBP3-45166] - Immunohistochemistry of paraffin-embedded human liver injury tissue slide using ( TOB2 Antibody) at dilution of 1:100")
![Western Blot: TOB2 Antibody - BSA Free [NBP3-45166] - MCF7 cells were subjected to SDS PAGE followed by western blot with ( TOB2 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-45166_rabbit-tob2-pab-bsa-free-284202511352262.jpg)
![Western Blot: Cullin 1 Antibody (S05-3D2) [NBP3-19613] - Western blot detection of Cullin 1 in K562, C6 cell lysates using NBP3-19613 (1:1000 diluted). Predicted band size: 90kDa. Observed band size: 90kDa.](http://images.novusbio.com/fullsize/Cullin-1-Antibody-S05-3D2-Western-Blot-NBP3-19613-img0002.jpg "Western Blot: Cullin 1 Antibody (S05-3D2) [NBP3-19613] - Western blot detection of Cullin 1 in K562, C6 cell lysates using NBP3-19613 (1:1000 diluted). Predicted band size: 90kDa. Observed band size: 90kDa.")
![Immunocytochemistry/Immunofluorescence: Cullin 1 Antibody (S05-3D2) [NBP3-19613] - Immunofluorescence of Cullin 1 (green) in Hela cells using NBP3-19613 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19613_rabbit-cullin-1-mab-s05-3d2-11320251555916.jpg)