| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72566 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03908-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59446-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04946-20ul | |||
![Western Blot: p16INK4a/CDKN2A Antibody (GT888) [NBP3-13670] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p16INK4a/CDKN2A antibody [888] (NBP3-13670) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-GT888-Western-Blot-NBP3-13670-img0002.jpg "Western Blot: p16INK4a/CDKN2A Antibody (GT888) [NBP3-13670] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p16INK4a/CDKN2A antibody [888] (NBP3-13670) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (GT888) [NBP3-13670] - Human breast. p16INK4a/CDKN2A stained by p16INK4a/CDKN2A antibody [888] (NBP3-13670) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-GT888-Immunohistochemistry-Paraffin-NBP3-13670-img0001.jpg)
![Western Blot: Miz-1/ZBTB17 Antibody (HL1548) - Azide and BSA Free [NBP3-25565] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Miz1 antibody [HL1548] (NBP3-25565) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25565_rabbit-miz-1-zbtb17-mab-hl1548-azide-and-bsa-free-2120249374939.jpg "Western Blot: Miz-1/ZBTB17 Antibody (HL1548) - Azide and BSA Free [NBP3-25565] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Miz1 antibody [HL1548] (NBP3-25565) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: Miz-1/ZBTB17 Antibody (HL1548) - Azide and BSA Free [NBP3-25565] - Miz1 antibody [HL1548] detects Miz1 protein by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Miz1 stained by Miz1 antibody [HL1548] (NBP3-25565) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25565_rabbit-miz-1-zbtb17-mab-hl1548-azide-and-bsa-free-2120249422690.jpg)
![Western Blot: MAD2L1-binding protein Antibody (3C11) [NBP2-45705] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAD2L1- binding protein.](http://images.novusbio.com/fullsize/MAD2L1-binding-protein-Antibody-3C11-Western-Blot-NBP2-45705-img0002.jpg "Western Blot: MAD2L1-binding protein Antibody (3C11) [NBP2-45705] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAD2L1- binding protein.")
![Immunohistochemistry: MAD2L1-binding protein Antibody (3C11) [NBP2-45705] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MAD2L1-binding-protein-Antibody-3C11-Immunohistochemistry-NBP2-45705-img0001.jpg)
![Western Blot: MORF4L2 Antibody [NB300-803] - HEK293 overexpressing MORF4L2 and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/MORF4L2-Antibody-Western-Blot-NB300-803-img0005.jpg "Western Blot: MORF4L2 Antibody [NB300-803] - HEK293 overexpressing MORF4L2 and probed with (mock transfection in first lane).")
![Western Blot: MORF4L2 Antibody [NB300-803] - NB300-803 staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/MORF4L2-Antibody-Western-Blot-NB300-803-img0004.jpg)
![Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353] - Western blot analysis of extracts of various cell lines, using Chk1 antibody (NBP3-16353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Chk1-Antibody-0S3Q1-Western-Blot-NBP3-16353-img0002.jpg "Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353] - Western blot analysis of extracts of various cell lines, using Chk1 antibody (NBP3-16353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Chk1 Antibody (0S3Q1) [NBP3-16353] - Immunohistochemistry of paraffin-embedded human colon using Chk1 Rabbit mAb (NBP3-16353) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Chk1-Antibody-0S3Q1-Immunohistochemistry-Paraffin-NBP3-16353-img0001.jpg)
![Western Blot: HMGA1 Antibody [NBP3-03908] - Analysis of extracts of 293T cells, using HMGA1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/fullsize/HMGA1-Antibody-Western-Blot-NBP3-03908-img0003.jpg "Western Blot: HMGA1 Antibody [NBP3-03908] - Analysis of extracts of 293T cells, using HMGA1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.")
![Immunohistochemistry-Paraffin: HMGA1 Antibody [NBP3-03908] - Paraffin-embedded human stomach using HMGA1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HMGA1-Antibody-Immunohistochemistry-Paraffin-NBP3-03908-img0002.jpg)
![Western Blot: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Western blot analysis of extracts of HepG2 cells, using CDCA5 Rabbit mAb (NBP3-16318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CDCA5-Antibody-1Y1C9-Western-Blot-NBP3-16318-img0002.jpg "Western Blot: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Western blot analysis of extracts of HepG2 cells, using CDCA5 Rabbit mAb (NBP3-16318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Immunohistochemistry of paraffin-embedded human thyroid cancer using CDCA5 Rabbit mAb (NBP3-16318) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDCA5-Antibody-1Y1C9-Immunohistochemistry-Paraffin-NBP3-16318-img0001.jpg)
![Western Blot: Calpain S1 Antibody (1D11) [NBP2-59446] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calpain S1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A431 cell lysate Lane 3.: 293T cell lysate](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-1D11-Western-Blot-NBP2-59446-img0001.jpg "Western Blot: Calpain S1 Antibody (1D11) [NBP2-59446] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calpain S1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A431 cell lysate Lane 3.: 293T cell lysate")
![Immunocytochemistry/Immunofluorescence: Calpain S1 Antibody (1D11) [NBP2-59446] - Analysis of Calpain S1 in HeLa cells. The cell was stained with (1:100). The secondary antibody (green) was used Alexa Fluor 488.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-1D11-Immunocytochemistry-Immunofluorescence-NBP2-59446-img0002.jpg)
![Western Blot: Kif2a Antibody [NBP3-04946] - Western blot analysis of extracts of various cell lines, using Kif2a antibody (NBP3-04946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Kif2a-Antibody-Western-Blot-NBP3-04946-img0001.jpg "Western Blot: Kif2a Antibody [NBP3-04946] - Western blot analysis of extracts of various cell lines, using Kif2a antibody (NBP3-04946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: Kif2a Antibody [NBP3-04946] - Western blot analysis of extracts of various cell lines, using Kif2a antibody (NBP3-04946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Kif2a Antibody-Western Blot-NBP3-04946-img0002.jpg)
![Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-288202412442975.jpg "Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-29202410234274.jpg)
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0005.jpg "Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).")
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - 1B anti-P53 (phospho Ser392) Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0004.jpg)