| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99422-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98936-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97315-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12647-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26671-50ul | |||
![Western Blot: UBE2R1/CDC34 Antibody (1P10A8) [NBP3-16882] - Western blot analysis of extracts of various cell lines, using UBE2R1/CDC34 Rabbit mAb (NBP3-16882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-1P10A8-Western-Blot-NBP3-16882-img0001.jpg "Western Blot: UBE2R1/CDC34 Antibody (1P10A8) [NBP3-16882] - Western blot analysis of extracts of various cell lines, using UBE2R1/CDC34 Rabbit mAb (NBP3-16882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: CDC37 Antibody [NBP2-99422] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-99422-img0004.jpg "Western Blot: CDC37 Antibody [NBP2-99422] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 50 kDa")
![Immunoprecipitation: CDC37 Antibody [NBP2-99422] - CDC37 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg A431 Whole Cell Lysate4 ul anti-CDC37 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CDC37 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-99422-img0003.jpg)
![Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33073_mouse-uba3-mab-1b6-6-5-r-28820241419156.jpg "Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: CDC37 Antibody [NBP2-98936] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-98936-img0002.jpg "Western Blot: CDC37 Antibody [NBP2-98936] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 50 kDa")
![Immunoprecipitation: CDC37 Antibody [NBP2-98936] - CDC37 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate4 ul anti-CDC37 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CDC37 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-98936-img0001.jpg)
![Immunohistochemistry-Paraffin: AMSH/STAMBP Antibody [NBP2-97315] - Immunochemical staining of human AMSH/STAMBP in human prostate with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/AMSH-STAMBP-Antibody-Immunohistochemistry-Paraffin-NBP2-97315-img0002.jpg "Immunohistochemistry-Paraffin: AMSH/STAMBP Antibody [NBP2-97315] - Immunochemical staining of human AMSH/STAMBP in human prostate with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: AMSH/STAMBP Antibody [NBP2-97315] - Immunochemical staining of human AMSH/STAMBP in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/AMSH-STAMBP-Antibody-Immunohistochemistry-Paraffin-NBP2-97315-img0001.jpg)
![Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32641_rabbit-nedd4-mab-je35-58-278202416331277.jpg "Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Uev1a/UBE2V1 Antibody [NBP2-88550] - WB Suggested Anti-UBE2V1 Antibody. Titration: 1.0 ug/ml. Positive Control: Fetal Brain](http://images.novusbio.com/fullsize/Uev1a-UBE2V1-Antibody-Western-Blot-NBP2-88550-img0001.jpg "Western Blot: Uev1a/UBE2V1 Antibody [NBP2-88550] - WB Suggested Anti-UBE2V1 Antibody. Titration: 1.0 ug/ml. Positive Control: Fetal Brain")
![Western Blot: Uev1a/UBE2V1 Antibody [NBP2-88550] - Host: Rabbit. Target Name: UBE2V1. Sample Tissue: Human Jurkat Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Uev1a-UBE2V1-Antibody-Western-Blot-NBP2-88550-img0002.jpg)
![Western Blot: AZ1 Antibody [NBP3-12647] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:122 kDa](http://images.novusbio.com/fullsize/AZ1-Antibody-Western-Blot-NBP3-12647-img0001.jpg "Western Blot: AZ1 Antibody [NBP3-12647] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:122 kDa")
![Immunoprecipitation: AZ1 Antibody [NBP3-12647] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-AZI1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AZI1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 122 kDaObserved band size :130 kDai](http://images.novusbio.com/fullsize/AZ1-Antibody-Immunoprecipitation-NBP3-12647-img0002.jpg)
![Western Blot: CDC37 Antibody [NBP1-70346] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-CDC37 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CDC37 was also immunoprecipitated by another rabbit anti-CDC37 antibody , which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP1-70346-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CDC37 Antibody [NBP1-70346] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-CDC37 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CDC37 was also immunoprecipitated by another rabbit anti-CDC37 antibody , which recognizes an upstream epitope.")
![Western Blot: CDC37 Antibody (10C3) [NBP3-26671] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate. All lanes: CDC37 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp3-26671_rabbit-cdc37-mab-10c3-28220241152525.jpg "Western Blot: CDC37 Antibody (10C3) [NBP3-26671] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate. All lanes: CDC37 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa Observed band size: 50 kDa")
![Flow Cytometry: CDC37 Antibody (10C3) [NBP3-26671] - Overlay histogram showing Hela cells stained with CDC37 Antibody (10C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26671_rabbit-cdc37-mab-10c3-282202414312749.jpg)
![Immunoprecipitation: USP16 Antibody [NB100-81639] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody used for IP at 6 ug per reaction. USP16 was also immunoprecipitated by rabbit anti-USP16 antibody. For blotting immunoprecipitated USP16 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunoprecipitation-NB100-81639-img0001.jpg "Immunoprecipitation: USP16 Antibody [NB100-81639] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody used for IP at 6 ug per reaction. USP16 was also immunoprecipitated by rabbit anti-USP16 antibody. For blotting immunoprecipitated USP16 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")