| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48588-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71389 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05409-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92649-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92972-0.02ml | |||
![Immunocytochemistry/Immunofluorescence: Septin-5 Antibody [NBP2-48588] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48588-img0003.jpg "Immunocytochemistry/Immunofluorescence: Septin-5 Antibody [NBP2-48588] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Septin-5 Antibody [NBP2-48588] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Septin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-48588-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-5 Antibody [NBP2-48588] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Septin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-48588-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Septin-5 Antibody [NBP2-48588] - Staining in human cerebral cortex and liver tissues using anti-SEPT5 antibody. Corresponding SEPT5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-48588-img0006.jpg)
![Western Blot: MCM2 Antibody (JE42-39) [NBP3-32577] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Mouse thymus tissue lysate (40 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: C2C12 cell lysate (20 ug/Lane) Lane 9: U-87 MG cell lysate (20 ug/Lane) Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32577_rabbit-mcm2-mab-je42-39-278202415212456.jpg "Western Blot: MCM2 Antibody (JE42-39) [NBP3-32577] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Mouse thymus tissue lysate (40 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: C2C12 cell lysate (20 ug/Lane) Lane 9: U-87 MG cell lysate (20 ug/Lane) Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM2 Antibody (JE42-39) [NBP3-32577] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32577) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32577_rabbit-mcm2-mab-je42-39-298202414262652.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (JE42-39) [NBP3-32577] - Immunocytochemistry analysis of HeLa cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/250 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32577_rabbit-mcm2-mab-je42-39-29202410484867.jpg)
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody NB100-1545 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-Western-Blot-NB100-1545-img0004.jpg "Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody NB100-1545 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Whole cell lysate (5 and 15 ug) from HeLa cells. Antibody used at 0.04 ug/ml for WB.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-Western-Blot-NB100-1545-img0003.jpg)
![Western Blot: SRPK1 Antibody [NBP1-30108] - Detection of human and mouse SRPK1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRPK1 antibody NBP1-30108 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SRPK1 was also immunoprecipitated by another rabbit anti-SRPK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/SRPK1-Antibody-Western-Blot-NBP1-30108-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SRPK1 Antibody [NBP1-30108] - Detection of human and mouse SRPK1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRPK1 antibody NBP1-30108 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SRPK1 was also immunoprecipitated by another rabbit anti-SRPK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-30108] - Section of human testicular seminoma. Antibody: Affinity purified rabbit anti- SRPK1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SRPK1-Antibody-Immunohistochemistry-NBP1-30108-img0002.jpg)
![Western Blot: MCM4 Antibody [NB100-290] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.](http://images.novusbio.com/fullsize/MCM4-Antibody-Western-Blot-NB100-290-img0002.jpg "Western Blot: MCM4 Antibody [NB100-290] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.")
![Immunohistochemistry: MCM4 Antibody [NB100-290] - Sample: FFPE section of human testicular seminoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-MCM4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-NB100-290-img0009.jpg)
![Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301] - Wild-type (WT) and CDK2 (phospho Thr160) knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Western-Blot-NBP3-13301-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301] - Wild-type (WT) and CDK2 (phospho Thr160) knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-13301] - CDK2 (phospho Thr160) antibody detects CDK2 (phospho Thr160) protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon. CDK2 (phospho Thr160) stained by CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Immunohistochemistry-Paraffin-NBP3-13301-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Western-Blot-NBP3-13301-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-13301] - CDK2 (phospho Thr160) antibody detects CDK2 (phospho Thr160) protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CDK2 (phospho Thr160) stained by CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Immunohistochemistry-Paraffin-NBP3-13301-img0002.jpg)
![Western Blot: CDKL1 Antibody (1D9) [NBP2-46217] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1.](http://images.novusbio.com/fullsize/CDKL1-Antibody-1D9-Western-Blot-NBP2-46217-img0003.jpg "Western Blot: CDKL1 Antibody (1D9) [NBP2-46217] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1.")
![Immunohistochemistry: CDKL1 Antibody (1D9) [NBP2-46217] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/CDKL1-Antibody-1D9-Immunohistochemistry-NBP2-46217-img0002.jpg)
![Immunohistochemistry: CDKL1 Antibody (1D9) [NBP2-46217] - Analysis of Human prostate Carcinoma tissue.](http://images.novusbio.com/fullsize/CDKL1-Antibody-1D9-Immunohistochemistry-NBP2-46217-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-05409] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CDK2 antibody (NBP3-05409).](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Immunohistochemistry-Paraffin-NBP3-05409-img0001.jpg "Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-05409] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CDK2 antibody (NBP3-05409).")
![Western Blot: CDK2 [p Thr160] Antibody [NBP3-05409] - analysis of C6, using Phospho-CDK2-T160 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05409_rabbit-polyclonal-cdk2-p-thr160-antibody-western-blot-159202383355..jpg)
![Western Blot: CDK2 [p Thr160] Antibody - Azide and BSA Free [CDK2] - Western blot analysis of lysates from C6 cells using CDK2 Rabbit pAb at 1:1000 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-05409_rabbit-polyclonal-cdk2-p-thr160-antibody-52202516345255.jpg)
![Western Blot: Cyclin G Antibody [NBP2-92649] - Analysis of extracts of various cell lines, using Cyclin G1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Cyclin-G-Antibody-Western-Blot-NBP2-92649-img0004.jpg "Western Blot: Cyclin G Antibody [NBP2-92649] - Analysis of extracts of various cell lines, using Cyclin G1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Cyclin G Antibody [NBP2-92649] - Rat testis using Cyclin G1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Cyclin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-92649-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyclin G Antibody [NBP2-92649] - Mouse testis using Cyclin G .mouse testis using Cyclin G1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Cyclin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-92649-img0002.jpg)
![Western Blot: CDK8 Antibody [NBP2-92972] - Analysis of 200ug extracts of Jurkat cells, using CDK8 antibody . Western blot was performed from the immunoprecipitate using this antibody](http://images.novusbio.com/fullsize/CDK8-Antibody-Western-Blot-NBP2-92972-img0004.jpg "Western Blot: CDK8 Antibody [NBP2-92972] - Analysis of 200ug extracts of Jurkat cells, using CDK8 antibody . Western blot was performed from the immunoprecipitate using this antibody")
![Western Blot: CDK8 Antibody [NBP2-92972] - Analysis of extracts of Jurkat cells, using CDK8 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .](http://images.novusbio.com/fullsize/CDK8-Antibody-Western-Blot-NBP2-92972-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CDK8 Antibody [NBP2-92972] - Analysis of extracts from normal (control) and CDK8 knockout (KO) HeLa cells, using CDK8 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/CDK8-Antibody-Knockout-Validated-NBP2-92972-img0005.jpg)
![Western Blot: CDKL1 Antibody (OTI1D9) - Azide and BSA Free [NBP2-71389] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1.](http://images.novusbio.com/fullsize/CDKL1-Antibody-OTI1D9-Azide-and-BSA-Free-Western-Blot-NBP2-71389-img0001.jpg "Western Blot: CDKL1 Antibody (OTI1D9) - Azide and BSA Free [NBP2-71389] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL1.")
![Immunohistochemistry: CDKL1 Antibody (OTI1D9) - Azide and BSA Free [NBP2-71389] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/CDKL1-Antibody-OTI1D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71389-img0002.jpg)
![Immunohistochemistry: CDKL1 Antibody (OTI1D9) - Azide and BSA Free [NBP2-71389] - Analysis of Human prostate Carcinoma tissue.](http://images.novusbio.com/fullsize/CDKL1-Antibody-OTI1D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71389-img0003.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)