| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92775-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15607-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74040-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38202-20ul | |||
![Western Blot: AKAP95/AKAP8 Antibody [NBP2-92775] - Analysis of extracts of various cell lines, using AKAP95/AKAP8 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-Western-Blot-NBP2-92775-img0002.jpg "Western Blot: AKAP95/AKAP8 Antibody [NBP2-92775] - Analysis of extracts of various cell lines, using AKAP95/AKAP8 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: AKAP95/AKAP8 Antibody - Azide and BSA Free [NBP2-92775] - Confocal immunofluorescence analysis of U2OS cells using AKAP95/AKAP8 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92775_rabbit-polyclonal-akap95-akap8-antibody-42202514275241.jpg%3Ft=1709198674)
![Western Blot: TRRAP Antibody [NBP3-15607] - Western blot analysis of extracts of various cell lines, using TRRAP antibody (NBP3-15607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/TRRAP-Antibody-Western-Blot-NBP3-15607-img0002.jpg "Western Blot: TRRAP Antibody [NBP3-15607] - Western blot analysis of extracts of various cell lines, using TRRAP antibody (NBP3-15607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: TRRAP Antibody [NBP3-15607] - Immunohistochemistry of paraffin-embedded human esophageal cancer using TRRAP Rabbit pAb (NBP3-15607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRRAP-Antibody-Immunohistochemistry-Paraffin-NBP3-15607-img0001.jpg)
![Western Blot: E2F6 Antibody (JE33-69) [NBP3-32286] - Western blot analysis of E2F6 on different lysates with Rabbit anti-E2F6 antibody (NBP3-32286) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32286) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32286_rabbit-e2f6-mab-je33-69-278202415212412.jpg "Western Blot: E2F6 Antibody (JE33-69) [NBP3-32286] - Western blot analysis of E2F6 on different lysates with Rabbit anti-E2F6 antibody (NBP3-32286) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32286) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E2F6 Antibody (JE33-69) [NBP3-32286] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-E2F6 antibody (NBP3-32286) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32286) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32286_rabbit-e2f6-mab-je33-69-298202414161835.jpg)
![Western Blot: DBC1/p30 Antibody [NB200-189] - Detection of Human and Mouse DBC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DBC1 antibody NB200-189 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/DBC1-p30-Antibody-Western-Blot-NB200-189-img0003.jpg "Western Blot: DBC1/p30 Antibody [NB200-189] - Detection of Human and Mouse DBC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DBC1 antibody NB200-189 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DBC1/p30 Antibody [NB200-189] - Detection of human DBC1/p30 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit DBC1/p30 Antibody NB200-189 (lot 2) used for IP at 6 ug per reaction. DBC1 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-DBC1 antibody NB200-190. For blotting immunoprecipitated DBC1, NB200-189 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/DBC1-p30-Antibody-Immunoprecipitation-NB200-189-img0004.jpg)
![Western Blot: TLK1 Antibody [NBP1-32354] - Sample(30 ug of whole cell lysate)A:293T 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/TLK1-Antibody-Western-Blot-NBP1-32354-img0004.jpg "Western Blot: TLK1 Antibody [NBP1-32354] - Sample(30 ug of whole cell lysate)A:293T 7. 5% SDS PAGE, antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: TLK1 Antibody [NBP1-32354] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/TLK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32354-img0003.jpg)
![Immunohistochemistry: HDAC9 Antibody (S01-8I6) [NBP3-19871] - Immunohistochemical of Human tonsil tissue using HDAC9 antibody at dilution 1/20](http://images.novusbio.com/fullsize/nbp3-19871_rabbit-hdac9-mab-s01-8i6-103202523464522.jpg "Immunohistochemistry: HDAC9 Antibody (S01-8I6) [NBP3-19871] - Immunohistochemical of Human tonsil tissue using HDAC9 antibody at dilution 1/20")
![Western Blot: AGO1/EIF2C1 Antibody [NBP1-56530] - THP-1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Western-Blot-NBP1-56530-img0002.jpg "Western Blot: AGO1/EIF2C1 Antibody [NBP1-56530] - THP-1 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: AGO1/EIF2C1 Antibody [NBP1-56530] - Jurkat, Antibody Dilution: 1.0 ug/ml AGO1 is supported by BioGPS gene expression data to be expressed in Jurkat.](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Western-Blot-NBP1-56530-img0003.jpg)
![Western Blot: CtBP1 Antibody (4D6) [NBP1-74040] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/CtBP1-Antibody-4D6-Western-Blot-NBP1-74040-img0001.jpg "Western Blot: CtBP1 Antibody (4D6) [NBP1-74040] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Western Blot: CtBP1 Antibody (4D6) [NBP1-74040] - The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: NIH3T3 cell lysate Lane 3.: 293T cell lysate Lane 4.: AGS cell lysateLane 5.: Raji cell lysateLane 6.: A549 cell lysateLane 7.: Jurkat cell lysate](http://images.novusbio.com/fullsize/CtBP1-Antibody-4D6-Western-Blot-NBP1-74040-img0002.jpg)
![Western Blot: CtBP1 Antibody (1I7G6) [NBP3-15412] - Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit mAb (NBP3-15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CtBP1-Antibody-1I7G6-Western-Blot-NBP3-15412-img0001.jpg "Western Blot: CtBP1 Antibody (1I7G6) [NBP3-15412] - Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit mAb (NBP3-15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CtBP1 Antibody (1I7G6) [NBP3-15412] - Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit mAb (NBP3-15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/CtBP1-Antibody-1I7G6-Western-Blot-NBP3-15412-img0002.jpg)
![Chromatin Immunoprecipitation: SUV39H2 Antibody [NBP3-38202] - Chromatin immunoprecipitation analysis of extracts of 293T cell line, using SUV39H2 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-38202_rabbit-suv39h2-pab-91220241418190.jpg "Chromatin Immunoprecipitation: SUV39H2 Antibody [NBP3-38202] - Chromatin immunoprecipitation analysis of extracts of 293T cell line, using SUV39H2 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.")
![Western Blot: SUV39H2 Antibody [NBP3-38202] - Western blot analysis of various lysates using SUV39H2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38202_rabbit-suv39h2-pab-912202414123832.jpg)