| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26186-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26404-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26367-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26246-50ul | |||
![Immunohistochemistry: c-jun Antibody (10D4) [NBP3-26186] - Image of c-jun Antibody (10D4) diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26186_rabbit-c-jun-mab-10d4-262202414471587.jpg "Immunohistochemistry: c-jun Antibody (10D4) [NBP3-26186] - Image of c-jun Antibody (10D4) diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: c-jun Antibody (10D4) [NBP3-26186] - Positive Western Blot detected in: 293 whole cell lysate. All lanes: c-jun Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 kDa Observed band size: 43 kDa](http://images.novusbio.com/fullsize/nbp3-26186_rabbit-c-jun-mab-10d4-28220241144263.jpg)
![Western Blot: BCAR3 Antibody [NB100-87003] - Detection of Human BCAR3 on HeLa whole cell lysate using NB100-87003. BCAR3 was also immunoprecipitated by another rabbit anti-BCAR3 antibody.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Western-Blot-NB100-87003-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BCAR3 Antibody [NB100-87003] - Detection of Human BCAR3 on HeLa whole cell lysate using NB100-87003. BCAR3 was also immunoprecipitated by another rabbit anti-BCAR3 antibody.")
![Western Blot: PCNP Antibody [NBP2-37692] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PCNP antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PCNP-Antibody-Western-Blot-NBP2-37692-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PCNP Antibody [NBP2-37692] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PCNP antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: PCNP Antibody [NBP2-37692] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PCNP antibody used for IP at 6 ug per reaction. PCNP was also immunoprecipitated by rabbit anti-PCNP antibody. For blotting immunoprecipitated PCNP, was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PCNP-Antibody-Immunoprecipitation-NBP2-37692-img0003.jpg)
![Western Blot: MDM2/HDM2 [p Ser185] Antibody [NB100-1683] - Shown in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/MDM2-HDM2 [p Ser185] Antibody-Western Blot-NB100-1683-img0003.jpg "Western Blot: MDM2/HDM2 [p Ser185] Antibody [NB100-1683] - Shown in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.")
![Western Blot: c-Myc [p Ser62] Antibody (2A10) [NBP3-26404] - Positive Western Blot detected in Hela whole cell lysate. All lanes: c-Myc [p Ser62] Antibody at 1.4ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57 KDa Observed band size: 57 KDa](http://images.novusbio.com/fullsize/nbp3-26404_rabbit-c-myc-p-ser-62-mab-2a10-282202411363014.jpg "Western Blot: c-Myc [p Ser62] Antibody (2A10) [NBP3-26404] - Positive Western Blot detected in Hela whole cell lysate. All lanes: c-Myc [p Ser62] Antibody at 1.4ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57 KDa Observed band size: 57 KDa")
![Immunocytochemistry/Immunofluorescence: c-Myc [p Ser62] Antibody (2A10) [NBP3-26404] - Staining of Hela cells with c-Myc [p Ser62] Antibody (2A10) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26404_rabbit-c-myc-p-ser-62-mab-2a10-282202412475135.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H4) [NBP3-26367] - Image of PRMT5 Antibody (2H4) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26367_rabbit-prmt5-mab-2h4-26220241535578.jpg "Immunohistochemistry: PRMT5 Antibody (2H4) [NBP3-26367] - Image of PRMT5 Antibody (2H4) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: PRMT5 Antibody (2H4) [NBP3-26367] - Positive Western Blot detected in: Raji whole cell lysate, Hela whole cell lysate, HL60 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate. All lanes: PRMT5 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 73, 72, 54, 68, 67 kDa Observed band size: 73 kDa](http://images.novusbio.com/fullsize/nbp3-26367_rabbit-prmt5-mab-2h4-28220241152517.jpg)
![Immunohistochemistry: 14-3-3 gamma Antibody (13C9) [NBP3-26246] - Image of 14-3-3 gamma Antibody (13C9) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26246_rabbit-14-3-3-gamma-mab-13c9-262202415355723.jpg "Immunohistochemistry: 14-3-3 gamma Antibody (13C9) [NBP3-26246] - Image of 14-3-3 gamma Antibody (13C9) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: 14-3-3 gamma Antibody (13C9) [NBP3-26246] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, K562 whole cell lysate. All lanes: 14-3-3 gamma Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29 kDa Observed band size: 29 kDa](http://images.novusbio.com/fullsize/nbp3-26246_rabbit-14-3-3-gamma-mab-13c9-282202411485818.jpg)