| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38501-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26260-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21543-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22663-50ul | |||
![Western Blot: RFC5 Antibody [NBP3-38501] - Western blot analysis of various lysates using RFC5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38501_rabbit-rfc5-pab-1012202411552827.jpg "Western Blot: RFC5 Antibody [NBP3-38501] - Western blot analysis of various lysates using RFC5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: Nbs1 Antibody (4G4R1) [NBP3-16355] - Western blot analysis of extracts of PC-3 cells, using Nbs1 Rabbit mAb (NBP3-16355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Nbs1-Antibody-4G4R1-Western-Blot-NBP3-16355-img0001.jpg "Western Blot: Nbs1 Antibody (4G4R1) [NBP3-16355] - Western blot analysis of extracts of PC-3 cells, using Nbs1 Rabbit mAb (NBP3-16355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: Nbs1 Antibody (4G4R1) [NBP3-16355] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Nbs1 antibody (NBP3-16355). Western blot was performed from the immunoprecipitate using Nbs1 antibody (NBP3-16355) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Nbs1-Antibody-4G4R1-Immunoprecipitation-NBP3-16355-img0002.jpg)
![Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32890_rabbit-rad50-mab-je60-04-288202412461633.jpg "Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunoprecipitation: SMG5 Antibody [NBP1-47272] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SMG5 antibody NBP1-47272 used for IP at 3 ug/mg lysate. SMG5 was also immunoprecipitated by rabbit anti-SMG5 antibody NBP1-47273, which recognizes a downstream epitope. For blotting immunoprecipitated SMG5, NBP1-47273 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMG5-Antibody-Immunoprecipitation-NBP1-47272-img0001.jpg "Immunoprecipitation: SMG5 Antibody [NBP1-47272] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SMG5 antibody NBP1-47272 used for IP at 3 ug/mg lysate. SMG5 was also immunoprecipitated by rabbit anti-SMG5 antibody NBP1-47273, which recognizes a downstream epitope. For blotting immunoprecipitated SMG5, NBP1-47273 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: USP22 Antibody (18E3) [NBP3-26260] - Staining of MCF-7 with USP22 Antibody (18E3) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 493-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26260_rabbit-usp22-mab-18e3-282202412475145.jpg "Immunocytochemistry/Immunofluorescence: USP22 Antibody (18E3) [NBP3-26260] - Staining of MCF-7 with USP22 Antibody (18E3) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 493-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: USP22 Antibody (18E3) [NBP3-26260] - Overlay Peak curve showing Hela cells stained with USP22 Antibody (18E3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26260_rabbit-usp22-mab-18e3-282202414183656.jpg)
![Western Blot: RFC4 Antibody [NB100-403] - Sample: Nuclear extract (20 ug for WB; 300 ug for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-RFC4 used at 0.5 ug/ml for WB and 2 ug/300 ug lysate for IP. Detection: Chemiluminescence with 10 sec. exposure.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NB100-403-img0001.jpg "Western Blot: RFC4 Antibody [NB100-403] - Sample: Nuclear extract (20 ug for WB; 300 ug for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-RFC4 used at 0.5 ug/ml for WB and 2 ug/300 ug lysate for IP. Detection: Chemiluminescence with 10 sec. exposure.")
![Western Blot: RPA2 [p Thr 21] Antibody (SR1535) [NBP3-21543] - Western blot analysis using NBP3-21543 in HeLa cell lysate treated with Calyculin A.](http://images.novusbio.com/fullsize/nbp3-21543_rabbit-rpa2-p-thr-21-mab-sr1535-57202317161847.jpg "Western Blot: RPA2 [p Thr 21] Antibody (SR1535) [NBP3-21543] - Western blot analysis using NBP3-21543 in HeLa cell lysate treated with Calyculin A.")
![Western Blot: Nbs1 [p Ser 343] Antibody (SR2302) [NBP3-22663] - Western blot analysis using NBP3-22663 in Jurkat cell lysate treated with Etopside.](http://images.novusbio.com/fullsize/nbp3-22663_rabbit-nbs1-p-ser-343-mab-sr2302-57202317215135.jpg "Western Blot: Nbs1 [p Ser 343] Antibody (SR2302) [NBP3-22663] - Western blot analysis using NBP3-22663 in Jurkat cell lysate treated with Etopside.")
![Immunoprecipitation: HUS1 Antibody (2B12) [H00003364-M01] - Analysis of HUS1 transfected lysate using anti-HUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HUS1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/HUS1-Antibody-2B12-Immunoprecipitation-H00003364-M01-img0001.jpg "Immunoprecipitation: HUS1 Antibody (2B12) [H00003364-M01] - Analysis of HUS1 transfected lysate using anti-HUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HUS1 MaxPab rabbit polyclonal antibody.")
![Sandwich ELISA: HUS1 Antibody (2B12) [H00003364-M01] - Detection limit for recombinant GST tagged HUS1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HUS1-Antibody-2B12-Sandwich-ELISA-H00003364-M01-img0002.jpg)
