| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35827-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59446-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59500-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38103-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59432-50ul | |||
![Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of various cell lines, using MDA5 antibody (NBP3-16132) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MDA5-Antibody-2N8Z5-Western-Blot-NBP3-16132-img0001.jpg "Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of various cell lines, using MDA5 antibody (NBP3-16132) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of BxPC-3 cells, using MDA5 antibody (NBP3-16132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MDA5-Antibody-2N8Z5-Western-Blot-NBP3-16132-img0002.jpg)
![Western Blot: Cdk5 Antibody (7C8T10) [Cdk5] - Western blot analysis of lysates from wild type(WT) and Cdk5 knockout (KO) 293T(KO) cells, using [KO Validated] Cdk5 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15620_rabbit-cdk5-mab-7c8t10-52202517352758.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cdk5 Antibody (7C8T10) [Cdk5] - Western blot analysis of lysates from wild type(WT) and Cdk5 knockout (KO) 293T(KO) cells, using [KO Validated] Cdk5 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk5 Antibody (7C8T10) [Cdk5] - Western blot analysis of lysates from Hela cells, using Cdk5 Rabbit mAb at 1:1000 dilutionSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15620_rabbit-cdk5-mab-7c8t10-5220251733380.jpg)
![Western Blot: CEND1 Antibody [NBP3-35827] - Western blot analysis of various lysates using CEND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35827_rabbit-cend1-pab-1012202411334026.jpg "Western Blot: CEND1 Antibody [NBP3-35827] - Western blot analysis of various lysates using CEND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: CEND1 Antibody [NBP3-35827] - Western blot analysis of various lysates using CEND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35827_rabbit-cend1-pab-1012202411404065.jpg)
![Western Blot: Calpain S1 Antibody (1D11) [NBP2-59446] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calpain S1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A431 cell lysate Lane 3.: 293T cell lysate](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-1D11-Western-Blot-NBP2-59446-img0001.jpg "Western Blot: Calpain S1 Antibody (1D11) [NBP2-59446] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calpain S1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A431 cell lysate Lane 3.: 293T cell lysate")
![Immunocytochemistry/Immunofluorescence: Calpain S1 Antibody (1D11) [NBP2-59446] - Analysis of Calpain S1 in HeLa cells. The cell was stained with (1:100). The secondary antibody (green) was used Alexa Fluor 488.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-1D11-Immunocytochemistry-Immunofluorescence-NBP2-59446-img0002.jpg)
![Western Blot: CtBP1 Antibody (1I7G6) [NBP3-15412] - Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit mAb (NBP3-15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CtBP1-Antibody-1I7G6-Western-Blot-NBP3-15412-img0001.jpg "Western Blot: CtBP1 Antibody (1I7G6) [NBP3-15412] - Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit mAb (NBP3-15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CtBP1 Antibody (1I7G6) [NBP3-15412] - Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit mAb (NBP3-15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/CtBP1-Antibody-1I7G6-Western-Blot-NBP3-15412-img0002.jpg)
![Western Blot: CSK Antibody (7C6) [NBP2-59500] - Lane 1: 293T cell lysates, Lane 2: CSK transfected 293T cell lysates](http://images.novusbio.com/fullsize/CSK-Antibody-7C6-Western-Blot-NBP2-59500-img0001.jpg "Western Blot: CSK Antibody (7C6) [NBP2-59500] - Lane 1: 293T cell lysates, Lane 2: CSK transfected 293T cell lysates")
![Immunocytochemistry/Immunofluorescence: CSK Antibody (7C6) [NBP2-59500] - Analysis of CSK in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CSK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/CSK-Antibody-7C6-Immunocytochemistry-Immunofluorescence-NBP2-59500-img0002.jpg)
![Western Blot: Tryptophanyl tRNA synthetase Antibody (3B2F10) [NBP3-16419] - Western blot analysis of extracts of various cell lines, using Tryptophanyl tRNA synthetase Rabbit mAb (NBP3-16419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-3B2F10-Western-Blot-NBP3-16419-img0002.jpg "Western Blot: Tryptophanyl tRNA synthetase Antibody (3B2F10) [NBP3-16419] - Western blot analysis of extracts of various cell lines, using Tryptophanyl tRNA synthetase Rabbit mAb (NBP3-16419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody (3B2F10) [NBP3-16419] - Immunohistochemistry of paraffin-embedded human placenta using Tryptophanyl tRNA synthetase Rabbit mAb (NBP3-16419) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-3B2F10-Immunohistochemistry-Paraffin-NBP3-16419-img0001.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Immunocytochemistry/ Immunofluorescence: NCAPH Antibody [NBP3-38103] - Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38103_rabbit-ncaph-pab-912202415161866.jpg "Immunocytochemistry/ Immunofluorescence: NCAPH Antibody [NBP3-38103] - Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: NCAPH Antibody [NBP3-38103] - Western blot analysis of various lysates using NCAPH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38103_rabbit-ncaph-pab-91220241418134.jpg)
![Western Blot: AIF Antibody (22E9) [NBP2-59432] - Lane 1: Mouse heart tissue lysates, Lane 2: Mouse liver tissue lysates](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-22E9-Western-Blot-NBP2-59432-img0001.jpg "Western Blot: AIF Antibody (22E9) [NBP2-59432] - Lane 1: Mouse heart tissue lysates, Lane 2: Mouse liver tissue lysates")
![Immunocytochemistry/Immunofluorescence: AIF Antibody (22E9) [NBP2-59432] - Analysis of AIFM1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AIFM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-22E9-Immunocytochemistry-Immunofluorescence-NBP2-59432-img0002.jpg)