| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26658-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-50081-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26414-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47852-0.1mg | |||
| IHC-Prediluted | NBP2-48278 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26493-50ul | |||
![Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-26220241510613.jpg "Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Chk1 Antibody (2F2) [NBP3-26658] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: Chk1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 44, 51 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-282202411442640.jpg)
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2F2) [NBP3-26658] - Staining of Hela Cells with Chk1 Antibody (2F2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-28220241323640.jpg)
![Western Blot: Villin 1 Antibody (VIL1/1314) [NBP2-53195] - A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1314).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-1314-Western-Blot-NBP2-53195-img0003.jpg "Western Blot: Villin 1 Antibody (VIL1/1314) [NBP2-53195] - A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1314).")
![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314) [NBP2-53195] - Formalin-fixed, paraffin-embedded human Rectum stained with Villin Monoclonal Antibody (VIL1/1314).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-1314-Immunohistochemistry-Paraffin-NBP2-53195-img0002.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314) [NBP2-53195] - Formalin-fixed, paraffin-embedded human Colon stained with Villin Monoclonal Antibody (VIL1/1314).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-1314-Immunohistochemistry-Paraffin-NBP2-53195-img0001.jpg)
![Western Blot: Retinoid X Receptor alpha Antibody [NBP2-20130] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/Retinoid-X-Receptor-alpha-Antibody-Western-Blot-NBP2-20130-img0001.jpg "Western Blot: Retinoid X Receptor alpha Antibody [NBP2-20130] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:5000.")
![Retinoid X Receptor alpha antibody immunoprecipitates RXRA protein in IP experiments.IP samples: MCF-7 whole cell extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of RXRA protein by 4 ug Retinoid X Receptor alpha antibody (NBP2-20130)7.5 % SDS-PAGEThe immunoprecipitated RXRA protein was detected by Retinoid X Receptor alpha antibody (NBP2-20130) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-20130_rabbit-polyclonal-rxr-alpha-nr2b1-antibody-immunoprecipitation-2611202518425411.jpg)
![Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Image of c-jun [p Ser63] Antibody (4A11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-2722024850463.jpg "Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Image of c-jun [p Ser63] Antibody (4A11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: c-jun [p Ser63] Antibody at 0.95 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48 KDa Observed band size: 48 KDa](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-282202411472127.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Staining of A549 cells with c-jun [p Ser63] Antibody (4A11) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-28220241312248.jpg)
![Western Blot: Bcl-2 Antibody (BCL2/796) [NBP2-44784] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (BCL2/796).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-796-Western-Blot-NBP2-44784-img0003.jpg "Western Blot: Bcl-2 Antibody (BCL2/796) [NBP2-44784] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (BCL2/796).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/796) [NBP2-44784] - Human Tonsil stained with Bcl-2 Monoclonal Antibody (BCL2/796).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-796-Immunohistochemistry-Paraffin-NBP2-44784-img0002.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/796) [NBP2-44784] - Human Melanoma stained with Bcl-2 Monoclonal Antibody (BCL2/796).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-796-Immunohistochemistry-Paraffin-NBP2-44784-img0001.jpg)
![Western Blot: HDGF Antibody [NBP1-71925] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-HDGF antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies NBP1-71924 and NBP1-71926, which recognize other epitopes.](http://images.novusbio.com/fullsize/HDGF-Antibody-Western-Blot-NBP1-71925-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: HDGF Antibody [NBP1-71925] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-HDGF antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies NBP1-71924 and NBP1-71926, which recognize other epitopes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: HDGF Antibody [NBP1-71925] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/HDGF-Antibody-Immunohistochemistry-NBP1-71925-img0004.jpg)
![Immunohistochemistry: HDGF Antibody [NBP1-71925] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/HDGF-Antibody-Immunohistochemistry-NBP1-71925-img0003.jpg)
![Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug). Lane 3: PC3 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-262202413221524.jpg "Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug). Lane 3: PC3 whole cell lysate (20ug).")
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-26220241547561.jpg)
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-2722024845325.jpg)
![Western Blot: ATM Antibody (4E11) [NBP3-26493] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate. All lanes: ATM Antibody at 2.05ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 350 KDa Observed band size: 350 KDa](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-28220241140928.jpg)
![Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Western-Blot-NBP2-15909-img0002.jpg "Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CKII alpha Antibody [NBP2-15909] - Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15909-img0003.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NBP2-15909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (NBP2-15909) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15909_rabbit-polyclonal-casein-kinase-2-alpha-antibody-91020242014672.jpg)
![Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - Panel A: Control antibody reactive to all forms of E2F (arrowheads). Panel B: Reactivity with phosphorylated E2F-1 (arrows) using E2F-1 [p Ser364] antibody. Lysate cells: CRE/E2F-1 CRE (human E2F-1 transfected mouse NIH3T3); NIH-3T3 (-ve control), & MDA-MB-231. Each lysate prepared from untreated cells & cells with 2 uM Doxorubicin. MDA-MB-231 also treated with genistein. Same membrane probed with E2F-1 [p Ser364] antibody @ 1:250 dilution, then stripped & re-probed with pan E2F antibody (+ve control). +ve control antibody shows an E2F-1 band in all human cell lines, but not mouse cells. Doxorubicin increases expression of E2F-1 (Panel A). Images overlapped to confirm that specific anti-E2F-1 pS364 staining shown in treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 for 45 min at RT for ECL detection.](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Western-Blot-NB600-900-img0007.jpg "Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - Panel A: Control antibody reactive to all forms of E2F (arrowheads). Panel B: Reactivity with phosphorylated E2F-1 (arrows) using E2F-1 [p Ser364] antibody. Lysate cells: CRE/E2F-1 CRE (human E2F-1 transfected mouse NIH3T3); NIH-3T3 (-ve control), & MDA-MB-231. Each lysate prepared from untreated cells & cells with 2 uM Doxorubicin. MDA-MB-231 also treated with genistein. Same membrane probed with E2F-1 [p Ser364] antibody @ 1:250 dilution, then stripped & re-probed with pan E2F antibody (+ve control). +ve control antibody shows an E2F-1 band in all human cell lines, but not mouse cells. Doxorubicin increases expression of E2F-1 (Panel A). Images overlapped to confirm that specific anti-E2F-1 pS364 staining shown in treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 for 45 min at RT for ECL detection.")
![Immunohistochemistry: E2F-1 [p Ser364] Antibody [NB600-900] - Affinity Purified E2F-1 [p Ser364] antibody was used at a 10 ug/ml to detect nuclear and occasionally cytoplasmic signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear staining between individual tumors, with some tumors showing strong staining. This image shows E2F-1 pS364 staining of human breast carcinoma. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Immunohistochemistry-NB600-900-img0008.jpg)
![Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - NB600-900 Western blot - E2F1 (phospho S364) antibody](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Western-Blot-NB600-900-img0001.jpg)
![Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NB100-593-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ROCK2 Antibody [NB100-593] - Detection of Human Rho Kinase Alpha on HeLa whole cell lysate using NB100-593. NB100-593 and NB100-592 were used at 5 ug/mg lysate to immunoprecipitate Rho Kinase Alpha.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NB100-593-img0002.jpg)
![Western Blot: DDX3 Antibody [NB200-196] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-196 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-194 and NB 200-195 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-196-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX3 Antibody [NB200-196] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-196 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-194 and NB 200-195 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).")
![Western Blot: ATP5D Antibody [NBP1-32365] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ATP5D-Antibody-Western-Blot-NBP1-32365-img0005.jpg "Western Blot: ATP5D Antibody [NBP1-32365] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ATP5D Antibody [NBP1-32365] - Paraffin-embedded breast cancer. ATP synthase delta antibody dilution: 1:250.](http://images.novusbio.com/fullsize/ATP5D-Antibody-Immunohistochemistry-Paraffin-NBP1-32365-img0006.jpg)
![Western Blot: ATP5D Antibody [NBP1-32365] -HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5D antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32365_rabbit-polyclonal-atp5d-antibody-western-blot-8220249370..jpg)