| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81045-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20097-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20095-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75899SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35895-100ug | |||
| Carrier-Free | NBP2-35895-500ug | |||
| Carrier-Free | NBP2-35895-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81113-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11989-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12029-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20102-0.025mg | |||
![Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-BBM-1-Chimeric-Western-Blot-NBP2-81045-img0003.jpg "Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.")
![Flow Cytometry: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of BBM.1 (NBP2-81045, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-BBM-1-Chimeric-Flow-Cytometry-NBP2-81045-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free [NBP2-81045] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-81045 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81045, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81045_rabbit-monoclonal-beta-2-microglobulin-antibody-bbm-1-chimeric-74202522113026.png)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0003.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0001.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0002.jpg)
![In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-In-vitro-assay-NBP3-14783-img0001.jpg "In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence")
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant Human SOD1-Cu-Zn SOD Pre-formed Fibrils Protein-Electron Microscopy-NBP3-14783-img0003.jpg)
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP3-14783-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28B-8 (NBP3-20097) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20097, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-2742023924237.png "Immunocytochemistry/Immunofluorescence: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28B-8 (NBP3-20097) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20097, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Western Blot: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Nuclear lysate of MCF7 cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-28B-8 (NBP3-20097) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-274202392428.png)
![Flow Cytometry: hHR23b Antibody (SAIC-28B-8) - Azide and BSA Free [NBP3-20097] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-28B-8 (NBP3-20097, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20097_rabbit-hhr23b-mab-saic-28b-8-azide-and-bsa-free-274202396466.png)
![Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-21C-4 (NBP3-20095) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20095, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-274202392427.png "Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-21C-4 (NBP3-20095) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20095, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-21C-4 (NBP3-20095, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-2742023924282.png)
![Western Blot: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Human colon tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-21C-4 (NBP3-20095) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-2742023924210.png)
![Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] - Western blot analysis of a human recombinant GSK-3 beta N-terminal fragment (lanes 1 & 2) and endogenous GSK-3 beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3 beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23366_mouse-gsk-3-beta-mab-m131-1992023199282.jpg "Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] - Western blot analysis of a human recombinant GSK-3 beta N-terminal fragment (lanes 1 & 2) and endogenous GSK-3 beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3 beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (M131) [NBP3-23366] - Immunocytochemical labeling of GSK-3 beta in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK-3 beta (NBP3-23366) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23366_mouse-gsk-3-beta-mab-m131-19920231252364.jpg)
![Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) [NBP2-75899] - Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b (NBP2-75899) at 1.5 ug/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. NBP2-75899 successfully detected CD52 in mouse spleen samples.](http://images.novusbio.com/fullsize/CD52-Antibody-YTH-34-5-G2b-Campath-1G-Western-Blot-NBP2-75899-img0001.jpg "Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) [NBP2-75899] - Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b (NBP2-75899) at 1.5 ug/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. NBP2-75899 successfully detected CD52 in mouse spleen samples.")
![Functional: Human IL1 beta Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL1-beta-Protein-Functional-NBP2-35895-img0001.jpg "Functional: Human IL1 beta Protein [NBP2-35895]")
![SDS-Page: Human IL-1 beta/IL-1F2 Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL-1-beta-IL-1F2-Protein-SDS-Page-NBP2-35895-img0002.jpg)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Western-Blot-NBP2-81113-img0002.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Flow-cytometry using the Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (NBP2-81113, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Flow-Cytometry-NBP2-81113-img0003.jpg)
![Immunocytochemistry /Immunofluorescence: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric - Azide and BSA Free [NBP2-81113] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with NBP2-81113 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81113, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81113_rabbit-monoclonal-fas-tnfrsf6-cd95-antibody-r-125224-chimeric-74202522141233.png)
![Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TIM-3-Antibody-2C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11989-img0001.jpg "Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![ELISA: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Binding curves of NBP3-11989 (blue line) and isotype control (NBP2-52638; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using NBP3-11989. For signal detection, anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113036.png)
![Flow Cytometry: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11989 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113017.png)
![Western Blot: 4-1BB/TNFRSF9/CD137 Antibody (4B4-1-1) - Chimeric [NBP3-12029] - Human thymus(A), lymph node(B) and tonsil(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 4B4-1-1 (NBP3-12029) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/4-1BB-TNFRSF9-CD137-Antibody-4B4-1-1-Chimeric-Western-Blot-NBP3-12029-img0001.jpg "Western Blot: 4-1BB/TNFRSF9/CD137 Antibody (4B4-1-1) - Chimeric [NBP3-12029] - Human thymus(A), lymph node(B) and tonsil(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 4B4-1-1 (NBP3-12029) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: 4-1BB/TNFRSF9/CD137 Antibody (4B4-1-1) - Chimeric [NBP3-12029] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 4B4-1-1 (NBP3-12029) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12029, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/4-1BB-TNFRSF9-CD137-Antibody-4B4-1-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12029-img0002.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (SAIC-34B-124) - Azide and BSA Free [NBP3-20102] - HepG2(A) (0.003ug/ml), HeLa(B) (0.003ug/ml), MCF7(C) (0.003ug/ml), K562(D) (0.003ug/ml) and LNCaP (E) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-34B-124 (NBP3-20102), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20102_rabbit-peroxiredoxin-2-mab-saic-34b-124-azide-bsa-free-274202396464.png "Western Blot: Peroxiredoxin 2 Antibody (SAIC-34B-124) - Azide and BSA Free [NBP3-20102] - HepG2(A) (0.003ug/ml), HeLa(B) (0.003ug/ml), MCF7(C) (0.003ug/ml), K562(D) (0.003ug/ml) and LNCaP (E) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-34B-124 (NBP3-20102), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: Peroxiredoxin 2 Antibody (SAIC-34B-124) - Azide and BSA Free [NBP3-20102] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-34B-124 (NBP3-20102, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20102_rabbit-peroxiredoxin-2-mab-saic-34b-124-azide-bsa-free-2742023924230.png)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 2 Antibody (SAIC-34B-124) - Azide and BSA Free [NBP3-20102] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-34B-124 (NBP3-20102) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20102, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20102_rabbit-peroxiredoxin-2-mab-saic-34b-124-azide-bsa-free-2742023924259.png)