| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98941-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06073-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90119-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98336-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05805-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06560-50ul | |||
![Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-98941] - Immunochemical staining Methionine Aminopeptidase 2/METAP2 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Methionine-Aminopeptidase-2-METAP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98941-img0004.jpg "Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-98941] - Immunochemical staining Methionine Aminopeptidase 2/METAP2 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-98941] - Immunochemical staining Methionine Aminopeptidase 2/METAP2 in human prostatic carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Methionine-Aminopeptidase-2-METAP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98941-img0001.jpg)
![Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-98941] - Immunochemical staining Methionine Aminopeptidase 2/METAP2 in human thyroid cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Methionine-Aminopeptidase-2-METAP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98941-img0002.jpg)
![Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-98941] - Immunochemical staining Methionine Aminopeptidase 2/METAP2 in mouse small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Methionine-Aminopeptidase-2-METAP2-Antibody-Immunohistochemistry-Paraffin-NBP2-98941-img0003.jpg)
![Western Blot: NIRF Antibody [NBP2-86734] - Host: Rabbit. Target: UHRF2. Positive control (+): Hela (HL). Negative control (-): 293T (2T). Antibody concentration: 0.1ug/ml](http://images.novusbio.com/fullsize/NIRF-Antibody-Western-Blot-NBP2-86734-img0003.jpg "Western Blot: NIRF Antibody [NBP2-86734] - Host: Rabbit. Target: UHRF2. Positive control (+): Hela (HL). Negative control (-): 293T (2T). Antibody concentration: 0.1ug/ml")
![Immunohistochemistry: NIRF Antibody [NBP2-86734] - Human Heart](http://images.novusbio.com/fullsize/NIRF-Antibody-Immunohistochemistry-NBP2-86734-img0001.jpg)
![Western Blot: NIRF Antibody [NBP2-86734] - WB Suggested Anti-UHRF2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/NIRF-Antibody-Western-Blot-NBP2-86734-img0002.jpg)
![Western Blot: NIRF Antibody [NBP2-86734] - Host: Rabbit. Target Name: UHRF2. Sample Tissue: Human Hela Whole Cell. Antibody Dilution: 0.5ug/ml](http://images.novusbio.com/fullsize/NIRF-Antibody-Western-Blot-NBP2-86734-img0004.jpg)
![Western Blot: NMRAL1 Antibody [NBP3-06073] - Anti-NMRAL1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:33 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/NMRAL1-Antibody-Western-Blot-NBP3-06073-img0001.jpg "Western Blot: NMRAL1 Antibody [NBP3-06073] - Anti-NMRAL1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:33 kDa (We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: NMRAL1 Antibody [NBP3-06073] - Immunochemical staining of human NMRAL1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NMRAL1-Antibody-Immunohistochemistry-Paraffin-NBP3-06073-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMRAL1 Antibody [NBP3-06073] - Immunofluorescence staining of NMRAL1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06073 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06073_rabbit-polyclonal-nmral1-antibody-235202512205535.png)
![Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line](http://images.novusbio.com/fullsize/SOS1-Antibody-SOS-01-Western-Blot-NB110-68800-img0003.jpg "Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line")
![Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SOS1-Antibody-SOS-01-Immunocytochemistry-Immunofluorescence-NB110-68800-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SOS1-Antibody-SOS-01-Immunocytochemistry-Immunofluorescence-NB110-68800-img0004.jpg)
![Western Blot: Cathepsin D Antibody (010) [NBP2-90119] - Anti-Cathepsin D rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: SKBR3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:45 kDa. Observed band size:31 kDa.](http://images.novusbio.com/fullsize/Cathepsin-D-Antibody-010-Western-Blot-NBP2-90119-img0004.jpg "Western Blot: Cathepsin D Antibody (010) [NBP2-90119] - Anti-Cathepsin D rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: SKBR3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:45 kDa. Observed band size:31 kDa.")
![Immunohistochemistry-Paraffin: Cathepsin D Antibody (010) [NBP2-90119] - Immunochemical staining of human CTSD in human cirrhosis with rabbit monoclonal antibody (1:300, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cathepsin-D-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90119-img0003.jpg)
![Immunohistochemistry-Paraffin: Cathepsin D Antibody (010) [NBP2-90119] - Immunochemical staining of human CTSD in human lung cancer with rabbit monoclonal antibody (1:300, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cathepsin-D-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90119-img0001.jpg)
![<b>Genetic Strategies Validation. </b>NBP2-90119 used at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Cathepsin D knockout 293T Whole Cell Lysate. Lysates loaded at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 31 kDa.](http://images.novusbio.com/fullsize/nbp2-90119_rabbit-monoclonal-cathepsin-d-antibody-010-45202316595466.png)
![Western Blot: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (LCL-HLA-G1).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-4-Western-Blot-NB500-533-img0001.jpg "Western Blot: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (LCL-HLA-G1).")
![Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-278202415242658.jpg "Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Mouse brain tissue lysate (40 ug/Lane) Lane 7: Mouse thymus tissue lysate (40 ug/Lane) Lane 8: Rat cerebellum tissue lysate (40 ug/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: RCAN1 Antibody (JE62-71) [NBP3-32100] - Immunocytochemistry analysis of Jurkat cells labeling RCAN1 with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202410234244.jpg)
![Flow Cytometry: RCAN1 Antibody (JE62-71) [NBP3-32100] - Flow cytometric analysis of Jurkat cells labeling RCAN1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32100, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32100_rabbit-rcan1-mab-je62-71-29202416372121.jpg)
![Western Blot: FADD Antibody (JE45-25) [NBP3-32328] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32328) at 1/1,000 dilution. Lane 1: A431 cell lysate, 10 ug/Lane Lane 2: K-562 cell lysate, 10 ug/Lane Lane 3: Jurkat cell lysate, 10 ug/Lane Lane 4: MCF7 cell lysate, 10 ug/Lane Lane 5: HeLa cell lysate, 10 ug/Lane Lane 6: SK-Br-3 cell lysate, 10 ug/Lane Lane 7: SW480 cell lysate, 10 ug/Lane Lane 8: THP-1 cell lysate, 10 ug/Lane Lane 9: A549 cell lysate, 10 ug/Lane Lane 10: HepG2 cell lysate, 10 ug/Lane Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 90 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32328) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32328_rabbit-fadd-mab-je45-25-278202416331258.jpg "Western Blot: FADD Antibody (JE45-25) [NBP3-32328] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32328) at 1/1,000 dilution. Lane 1: A431 cell lysate, 10 ug/Lane Lane 2: K-562 cell lysate, 10 ug/Lane Lane 3: Jurkat cell lysate, 10 ug/Lane Lane 4: MCF7 cell lysate, 10 ug/Lane Lane 5: HeLa cell lysate, 10 ug/Lane Lane 6: SK-Br-3 cell lysate, 10 ug/Lane Lane 7: SW480 cell lysate, 10 ug/Lane Lane 8: THP-1 cell lysate, 10 ug/Lane Lane 9: A549 cell lysate, 10 ug/Lane Lane 10: HepG2 cell lysate, 10 ug/Lane Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 90 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32328) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FADD Antibody (JE45-25) [NBP3-32328] - Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-FADD antibody (NBP3-32328) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32328) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32328_rabbit-fadd-mab-je45-25-298202413585545.jpg)
![Immunocytochemistry/ Immunofluorescence: FADD Antibody (JE45-25) [NBP3-32328] - Immunocytochemistry analysis of SW480 cells labeling FADD with Rabbit anti-FADD antibody (NBP3-32328) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FADD antibody (NBP3-32328) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32328_rabbit-fadd-mab-je45-25-29202410484813.jpg)
![Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98336-img0004.jpg "Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-98336] - Immunochemical staining of mouse E-Cadherin in mouse stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-98336-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP2-98336] - Mouse E-Cadherin was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-Mouse E-Cadherin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse E-Cadherin rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band size: 130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP2-98336-img0001.jpg)
![Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Flow cytometric analysis of Human Park7/DJ-1 expression on HeLa cells. The cells were treated, stained with NBP3-05805, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-235202521451748.png "Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Flow cytometric analysis of Human Park7/DJ-1 expression on HeLa cells. The cells were treated, stained with NBP3-05805, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody (007) [NBP3-05805] - Immunofluorescence staining of Human Park7/DJ-1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-05805 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-23520251628125.png)
![Western Blot: Park7/DJ-1 Antibody (007) [NBP3-05805] - NBP3-05805 at 1:1000 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:20 kDa Observed band size:24 kDa](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-23520252135168.png)
![Immunoprecipitation: Park7/DJ-1 Antibody (007) [NBP3-05805] - PARK7 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg 293T Whole Cell Lysate 2 uL NBP3-05805 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-05805, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 20 kDa Observed band size: 20 kDa](http://images.novusbio.com/fullsize/nbp3-05805_rabbit-monoclonal-park7-dj-1-antibody-007-235202514331514.png)
![Western Blot: TRF-2 Antibody (JE37-57) [NBP3-33049] - Western blot analysis of TRF-2 on different lysates with Rabbit anti-TRF-2 antibody (NBP3-33049) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33049) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33049_rabbit-trf-2-mab-je37-57-288202412493422.jpg "Western Blot: TRF-2 Antibody (JE37-57) [NBP3-33049] - Western blot analysis of TRF-2 on different lysates with Rabbit anti-TRF-2 antibody (NBP3-33049) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33049) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TRF-2 Antibody (JE37-57) [NBP3-33049] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-TRF-2 antibody (NBP3-33049) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33049) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33049_rabbit-trf-2-mab-je37-57-298202414304128.jpg)
![Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (JE37-57) [NBP3-33049] - Immunocytochemistry analysis of SiHa cells labeling TRF-2 with Rabbit anti-TRF-2 antibody (NBP3-33049) at 20ug/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TRF-2 antibody (NBP3-33049) at 20ug/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33049_rabbit-trf-2-mab-je37-57-29202410334036.jpg)
![Western Blot: UBE2R1/CDC34 Antibody [NBP3-06560] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-Western-Blot-NBP3-06560-img0001.jpg "Western Blot: UBE2R1/CDC34 Antibody [NBP3-06560] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa")
![Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody [NBP3-06560] - Staining of human CDC34 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-Immunohistochemistry-Paraffin-NBP3-06560-img0002.jpg)
![Immunoprecipitation: UBE2R1/CDC34 Antibody [NBP3-06560] - Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-CDC34 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CDC34 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :26 kDa](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-Immunoprecipitation-NBP3-06560-img0003.jpg)