| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99548-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99115-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99896-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89383-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99469-50ul | |||
![Western Blot: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-27820241514176.jpg "Western Blot: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32021) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-298202413585574.jpg)
![Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Immunocytochemistry analysis of HeLa cells labeling ALDH2 with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-29202410234220.jpg)
![Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-28820241423310.jpg "Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-298202414262614.jpg)
![Flow Cytometry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Flow cytometric analysis of Jurkat cells labeling ZEB1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-2920241645327.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0005.jpg "Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0001.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0002.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0003.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0004.jpg)
![Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] - Immunochemical staining of human Methionine Aminopeptidase 2/METAP2 in human small intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Methionine-Aminopeptidase-2-METAP2-Antibody-Immunohistochemistry-Paraffin-NBP2-99115-img0004.jpg "Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] - Immunochemical staining of human Methionine Aminopeptidase 2/METAP2 in human small intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).")
![Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: PC12 Whole Cell LysateLysates/proteins at 30 μg per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/antibody/nbp2-99115_rabbit-polyclonal-methionine-aminopeptidase-2-metap2-antibody-western-blot-3052024113920..jpg)
![Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A:0.5 mg HepG2 Whole Cell Lysate4 µL anti-METAP2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-METAP2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 72 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99115_rabbit-polyclonal-methionine-aminopeptidase-2-metap2-antibody-immunoprecipitation-3052024114037..jpg)
![Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] - Lane A: METAP2 konckout Hela Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 10 μg per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99115_rabbit-polyclonal-methionine-aminopeptidase-2-metap2-antibody-western-blot-3052024114335..jpg)
![Western Blot: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Western blot analysis of p62/SQSTM1 on different lysates with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/500 dilution. Lane 1: PC-12 cell lysate (10 ug/Lane) Lane 2: RAW264.7 cell lysate (10 ug/Lane) Lane 3: PANC-1 cell lysate (10 ug/Lane) Lane 4: Mouse liver tissue lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32702) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32702_rabbit-p62-sqstm1-mab-ps00-61-278202415295572.jpg "Western Blot: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Western blot analysis of p62/SQSTM1 on different lysates with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/500 dilution. Lane 1: PC-12 cell lysate (10 ug/Lane) Lane 2: RAW264.7 cell lysate (10 ug/Lane) Lane 3: PANC-1 cell lysate (10 ug/Lane) Lane 4: Mouse liver tissue lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32702) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32702) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32702_rabbit-p62-sqstm1-mab-ps00-61-298202414161843.jpg)
![Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Immunocytochemistry analysis of A549 cells labeling p62/SQSTM1 with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32702_rabbit-p62-sqstm1-mab-ps00-61-29202410484897.jpg)
![Western Blot: AK2 Antibody [NBP2-99896] - Anti-AK2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 33 kDa](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP2-99896-img0005.jpg "Western Blot: AK2 Antibody [NBP2-99896] - Anti-AK2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 33 kDa")
![Immunohistochemistry-Paraffin: AK2 Antibody [NBP2-99896] - Immunochemical staining of human AK2 in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AK2-Antibody-Immunohistochemistry-Paraffin-NBP2-99896-img0004.jpg)
![Immunohistochemistry-Paraffin: AK2 Antibody [NBP2-99896] - Immunochemical staining of human AK2 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AK2-Antibody-Immunohistochemistry-Paraffin-NBP2-99896-img0003.jpg)
![Immunoprecipitation: AK2 Antibody [NBP2-99896] - AK2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 2 ul anti-AK2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-AK2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa](http://images.novusbio.com/fullsize/AK2-Antibody-Immunoprecipitation-NBP2-99896-img0001.jpg)
![Western Blot: NUT Antibody (PSH03-36) [NBP3-32674] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32674) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-hNutm1 cell lysate Lysates/proteins at 20 ug/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32674) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32674_rabbit-nut-mab-psh03-36-27820241631613.jpg "Western Blot: NUT Antibody (PSH03-36) [NBP3-32674] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32674) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-hNutm1 cell lysate Lysates/proteins at 20 ug/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32674) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUT Antibody (PSH03-36) [NBP3-32674] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32674) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32674) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32674_rabbit-nut-mab-psh03-36-29820241420983.jpg)
![Immunocytochemistry/ Immunofluorescence: NUT Antibody (PSH03-36) [NBP3-32674] - Immunocytochemistry analysis of HeLa transfacted with or without human NUT cells labeling Nutm1 with Rabbit anti-NUT antibody (NBP3-32674) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUT antibody (NBP3-32674) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32674_rabbit-nut-mab-psh03-36-2920241192336.jpg)
![Western Blot: Galectin-3 Antibody (024) [NBP2-89383] - Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Western-Blot-NBP2-89383-img0003.jpg "Western Blot: Galectin-3 Antibody (024) [NBP2-89383] - Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (024) [NBP2-89383] - Staining of Human Galectin3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human Galectin3 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nuclear and cytoplasm.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Immunocytochemistry-Immunofluorescence-NBP2-89383-img0001.jpg)
![Flow Cytometry: Galectin-3 Antibody (024) [NBP2-89383] - Analysis of Human Galectin3 expression on HeLa cells. The cells were treated stained with purified anti-Human Galectin3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Flow-Cytometry-NBP2-89383-img0002.jpg)
![Immunoprecipitation: Galectin-3 Antibody (024) [NBP2-89383] - Lane A:0.5 mg mouse pancreas Whole Cell Lysate 4 uL anti-NKX3-1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody: Anti-NKX3-1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique. Performed under reducing conditions.Predicted band size: 27 kDa Observed band size :30 kDa](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Immunoprecipitation-NBP2-89383-img0004.jpg)
![Western Blot: COX17 Antibody [NBP2-99469] - Anti-COX17 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 7 kDa](http://images.novusbio.com/fullsize/COX17-Antibody-Western-Blot-NBP2-99469-img0006.jpg "Western Blot: COX17 Antibody [NBP2-99469] - Anti-COX17 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 7 kDa")
![Immunohistochemistry-Paraffin: COX17 Antibody [NBP2-99469] - Immunochemical staining of human COX17 in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/COX17-Antibody-Immunohistochemistry-Paraffin-NBP2-99469-img0004.jpg)
![Immunohistochemistry-Paraffin: COX17 Antibody [NBP2-99469] - Immunochemical staining of human COX17 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/COX17-Antibody-Immunohistochemistry-Paraffin-NBP2-99469-img0003.jpg)
![Immunoprecipitation: COX17 Antibody [NBP2-99469] - COX17 was immunoprecipitated using: Lane A: 0.5 mg mouse kidney tissue Lysate 4 ul anti-COX17 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-COX17 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 88 kDa. Observed band size: 130 kDa](http://images.novusbio.com/fullsize/COX17-Antibody-Immunoprecipitation-NBP2-99469-img0005.jpg)
![Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-288202412461668.jpg "Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32914) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-298202414262644.jpg)
![Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunocytochemistry analysis of HeLa cells labeling ROCK2 with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-29202410334061.jpg)
![Western Blot: AG-3/AGR3 Antibody (AGR3.1) [NBP1-45004] - Analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.](http://images.novusbio.com/fullsize/AG-3-AGR3-Antibody-AGR3-1-Western-Blot-NBP1-45004-img0002.jpg "Western Blot: AG-3/AGR3 Antibody (AGR3.1) [NBP1-45004] - Analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.")
![Immunohistochemistry: AG-3/AGR3 Antibody (AGR3.1) [NBP1-45004] - Staining of AGR3 with mouse monoclonal AGR3.1 in paraffin-embedded sections of breast carcinoma.](http://images.novusbio.com/fullsize/AG-3-AGR3-Antibody-AGR3-1-Immunohistochemistry-NBP1-45004-img0001.jpg)
![Western Blot: Mouse Monoclonal AG-3/AGR3 Antibody (AGR3.1) [NBP1-45004] - Western blotting analysis was performed on whole cell extracts (HOT lysis buffer) of MCF-7, HeLa, and Jurkat cell lines, mixed and heated (100C, 5 min) with reducing SDS-loading buffer. Samples were resolved using 15% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody AGR3.1 (1 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.AGR3 molecules were detected at ~ 14 kDa in MCF-7 cell line.](http://images.novusbio.com/fullsize/antibody/nbp1-45004_mouse-monoclonal-ag-3-agr3-antibody-agr3-1-western-blot-1112202411422..jpg)