| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27162-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48397 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31370SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92688SS | |||
![Western Blot: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Western blot analysis of UQCRC2 on different lysates with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33085) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33085_rabbit-uqcrc2-mab-je32-56-288202414191517.jpg "Western Blot: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Western blot analysis of UQCRC2 on different lysates with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33085) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33085_rabbit-uqcrc2-mab-je32-56-2982024149268.jpg)
![Immunocytochemistry/ Immunofluorescence: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Immunocytochemistry analysis of HepG2 cells labeling UQCRC2 with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33085_rabbit-uqcrc2-mab-je32-56-29202410234246.jpg)
![Flow Cytometry: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Flow cytometric analysis of HepG2 cells labeling UQCRC2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33085, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33085_rabbit-uqcrc2-mab-je32-56-29202416372169.jpg)
![Immunohistochemistry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32236) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-298202414304156.jpg "Immunohistochemistry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32236) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Immunocytochemistry analysis of Neuro-2a cells labeling Cyclin D1 with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cyclin D1 antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-29202410484833.jpg)
![Flow Cytometry: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Flow cytometric analysis of MCF7 cells labeling Cyclin D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32236, red) at 1/5,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-29202416453255.jpg)
![Western Blot: Cyclin D1 Antibody (PD01-64) [NBP3-32236] - Western blot analysis of Cyclin D1 on different lysates with Rabbit anti-Cyclin D1 antibody (NBP3-32236) at 1/5,000 dilution.Lane 1: MCF7 cell lysateLane 2: K-562 cell lysate (negative)Lane 3: A431 cell lysateLane 4: Neuro-2a cell lysateLane 5: NIH/3T3 cell lysateLane 6: C6 cell lysateLane 7: SH-SY5Y cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 34 kDaObserved band size: 35 kDaExposure time: 20 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32236_rabbit-cyclin-d1-mab-pd01-64-3920248485020.jpg)
![Western Blot: Galectin-7 Antibody (JB38-11) [NBP2-75500] - Analysis of Galectin 7 on human skin tissue lysate using anti-Galectin 7 antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Galectin-7-Antibody-JB38-11-Western-Blot-NBP2-75500-img0005.jpg "Western Blot: Galectin-7 Antibody (JB38-11) [NBP2-75500] - Analysis of Galectin 7 on human skin tissue lysate using anti-Galectin 7 antibody at 1:500 dilution.")
![Immunocytochemistry/Immunofluorescence: Galectin-7 Antibody (JB38-11) [NBP2-75500] - Staining Galectin 7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Galectin-7-Antibody-JB38-11-Immunocytochemistry-Immunofluorescence-NBP2-75500-img0002.jpg)
![Immunohistochemistry-Paraffin: Galectin-7 Antibody (JB38-11) [NBP2-75500] - Analysis of paraffin-embedded human skin tissue using anti-Galectin 7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Galectin-7-Antibody-JB38-11-Immunohistochemistry-Paraffin-NBP2-75500-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-7 Antibody (JB38-11) [NBP2-75500] - Staining Galectin 7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Galectin-7-Antibody-JB38-11-Immunocytochemistry-Immunofluorescence-NBP2-75500-img0001.jpg)
![Immunohistochemistry-Paraffin: Galectin-7 Antibody (JB38-11) [NBP2-75500] - Analysis of paraffin-embedded human tonsil tissue using anti-Galectin 7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Galectin-7-Antibody-JB38-11-Immunohistochemistry-Paraffin-NBP2-75500-img0003.jpg)
![Microarray: Galectin-7 Antibody (JB38-11) [NBP2-75500] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Galectin-7-Antibody-JB38-11-Microarray-NBP2-75500-img0007.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0006.jpg "Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.")
![Flow Cytometry: CD10 Antibody (MEM-78) [NB500-372]-Fig. 1. Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD10 (MEM-78) PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/p_444_1.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0001.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC-CyTM7.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0002.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-CyTM5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0003.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-DyLigt(R) 594.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0004.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0005.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Western blot analysis of alpha-Smooth Muscle Actin expression in human omentum, serous carcinoma, blotted with alpha-Smooth Muscle Actin Antibody (4A8-2H3). The band indicates an observed molecular weight of 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-Western-Blot-H00000059-M02-img0010.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Western blot analysis of alpha-Smooth Muscle Actin expression in human omentum, serous carcinoma, blotted with alpha-Smooth Muscle Actin Antibody (4A8-2H3). The band indicates an observed molecular weight of 42 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Immunofluorescence analysis of alpha-Smooth Muscle Actin on HeLa cell. Antibody concentration 1 - 10 ug/ml.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-Immunocytochemistry-Immunofluorescence-H00000059-M02-img0008.jpg)
![ELISA: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Detection limit for recombinant GST tagged alpha-Smooth Muscle Actin Antibody is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-ELISA-H00000059-M02-img0011.jpg)
![Western Blot: PSP94/MSMB Antibody (3B11) [H00004477-M08] - Validation of selected differentially expressed genes. Immunoblot analysis of selected gene products identified by microarray and RT-PCR as altered in drug resistant cells. Image collected and cropped by CiteAb from the following publication (//www.ovarianresearch.com/content/4/1/21), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PSP94-MSMB-Antibody-3B11-Western-Blot-H00004477-M08-img0002.jpg "Western Blot: PSP94/MSMB Antibody (3B11) [H00004477-M08] - Validation of selected differentially expressed genes. Immunoblot analysis of selected gene products identified by microarray and RT-PCR as altered in drug resistant cells. Image collected and cropped by CiteAb from the following publication (//www.ovarianresearch.com/content/4/1/21), licensed under a CC-BY license.")
![ELISA: PSP94/MSMB Antibody (3B11) [H00004477-M08] - Detection limit for recombinant GST tagged MSMB is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PSP94-MSMB-Antibody-3B11-ELISA-H00004477-M08-img0001.jpg)
![Western Blot: PSP94/MSMB Antibody (3B11) [H00004477-M08] - Validation of selected differentially expressed genes. A. RT-PCR analysis of genes elevated in drug resistant cells. The y-axis represents fold up-regulation in the different drug resistant cell lines over the parental OV90 cell line. B. RT-PCR analysis of genes decreased in drug resistant cells. The y-axis represents the fold down-regulation of the different resistant cell lines compared to the parental OV90 cell line. C. Immunoblot analysis of selected gene products identified by microarray & RT-PCR as altered in drug resistant cells. Image collected & cropped by CiteAb from the following publication (//ovarianresearch.biomedcentral.com/articles/10.1186/1757-2215-4-21), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004477-m08_mouse-monoclonal-psp94-msmb-antibody-3b11-310202415384111.jpg)
![Flow Cytometry: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis of SK-OV-3 cells using SP17 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-10420249141937.jpg%3Fv=1666704905 "Flow Cytometry: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis of SK-OV-3 cells using SP17 mouse mAb (green) and negative control (red).")
![Western Blot: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis using SP17 mAb against human SP17 (AA: 1-152) recombinant protein. (Expected MW is 20.3 kDa)](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-1042024920224.jpg%3Fv=1666704905)
![ELISA: SP17 Antibody (1C4C7) [NBP3-27162] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-10420249181945.jpg%3Fv=1666704905)
![Western Blot: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis using SP17 mAb against HEK293-6e (1) and SP17 (AA: 1-152)-hIgGFc transfected HEK293-6e (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-1042024917018.jpg%3Fv=1666704905)
![Immunohistochemistry: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis of paraffin-embedded testi tissues using SP17 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-1042024921155.jpg%3Fv=1666704905)
![Immunohistochemistry: SP17 Antibody (1C4C7) [NBP3-27162] - Analysis of paraffin-embedded endometrial cancer tissues using SP17 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27162_mouse-sp17-mab-1c4c7-bsa-free-1042024914197.jpg%3Fv=1666704905)
![Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Immunofluorescence Analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with Glypican 3 Antibody (SPM595) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47761-img0004.jpg "Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Immunofluorescence Analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with Glypican 3 Antibody (SPM595) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)")
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Human Melanoma stained with Glypican-3 Monoclonal Antibody (SPM595)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47761-img0003.jpg)
![Flow Cytometry: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Flow Cytometric Analysis of PFA-fixed HepG2 cells using Glypican 3 Antibody (SPM595) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47761-img0001.jpg)
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (SPM595)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47761-img0002.jpg)
![Western Blot: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Western-Blot-NBP2-54545-img0005.jpg "Western Blot: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).")
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54545-img0001.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54545-img0002.jpg)
![Protein Array: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TOP2A Antibody (TOP2A/1362). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Protein-Array-NBP2-54545-img0003.jpg)
![Western Blot: NUT Antibody [NBP2-31370] - WB detection of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein with NUT antibody at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 of NUT protein at ~120 kDa and ~46kDa respectively.](http://images.novusbio.com/fullsize/NUT-Antibody-Western-Blot-NBP2-31370-img0001.jpg "Western Blot: NUT Antibody [NBP2-31370] - WB detection of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein with NUT antibody at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 of NUT protein at ~120 kDa and ~46kDa respectively.")
![Immunocytochemistry/Immunofluorescence: NUT Antibody [NBP2-31370] - NUT antibody was tested at 0.01 ug/ml in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/NUT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31370-img0005.jpg)
![Western Blot: XPF Antibody [NB100-60679] - Detection of Human XPF/ERCC4 on HeLa whole cell lysate using NB100-60679.](http://images.novusbio.com/fullsize/XPF-Antibody-Western-Blot-NB100-60679-img0004.jpg "Western Blot: XPF Antibody [NB100-60679] - Detection of Human XPF/ERCC4 on HeLa whole cell lysate using NB100-60679.")
![Immunohistochemistry-Paraffin: XPF Antibody [NB100-60679] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti- XPF/ERCC4 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/XPF-Antibody-Immunohistochemistry-Paraffin-NB100-60679-img0007.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Knockout-Validated-NBP1-92688-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [NBP1-92688] - Immunocytochemistry/Immunofluorescence: [NBP1-92688] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Immunocytochemistry-Immunofluorescence-NBP1-92688-img0005.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Analysis of cell and whole brain tissue lysates using mouse mAb to vimentin, NBP1-92688, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Western-Blot-NBP1-92688-img0006.jpg)