| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58884-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21071 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52646-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-82221SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50172-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-49873-0.1mg | |||
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-58884] - Staining of human cell line RH-30 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58884-img0002.jpg "Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-58884] - Staining of human cell line RH-30 shows localization to intermediate filaments. Antibody staining is shown in green.")
![Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (20 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Src-[p-Tyr527]-Antibody-Western-Blot-NBP3-13245-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (20 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Src-[p-Tyr527]-Antibody-Western-Blot-NBP3-13245-img0003.jpg)
![SDS-Page: Recombinant Human CD20 Protein [NBP2-61455] - 15% SDS-Page (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-CD20-Protein-SDS-Page-NBP2-61455-img0001.jpg "SDS-Page: Recombinant Human CD20 Protein [NBP2-61455] - 15% SDS-Page (3ug)")
![Western Blot: Wnt-5a Antibody (3P2E8) [NBP3-15684] - Western blot analysis of extracts of various cell lines, using Wnt-5a antibody (NBP3-15684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Wnt-5a-Antibody-3P2E8-Western-Blot-NBP3-15684-img0002.jpg "Western Blot: Wnt-5a Antibody (3P2E8) [NBP3-15684] - Western blot analysis of extracts of various cell lines, using Wnt-5a antibody (NBP3-15684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Wnt-5a Antibody (3P2E8) [NBP3-15684] - Immunohistochemistry of paraffin-embedded human lung cancer using Wnt-5a Rabbit mAb (NBP3-15684) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Wnt-5a-Antibody-3P2E8-Immunohistochemistry-Paraffin-NBP3-15684-img0001.jpg)
![Western Blot: HIF-2 alpha Overexpression Lysate (Adult Normal) [NBL1-10286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF-2 alpha.](http://images.novusbio.com/fullsize/HIF-2-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10286-img0002.jpg "Western Blot: HIF-2 alpha Overexpression Lysate (Adult Normal) [NBL1-10286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF-2 alpha.")
![ELISA: Human Wnt-5a ELISA Kit (Colorimetric) [NBP3-38894] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38894_human-wnt-5a-elisa-kit-colorimetric-211020241535502.png "ELISA: Human Wnt-5a ELISA Kit (Colorimetric) [NBP3-38894] - Standard Curve Reference")
![Western Blot: c-Myc Antibody (PS00-11) [NBP3-32201] - Western blot analysis of c-Myc on different lysates with Rabbit anti-c-Myc antibody (NBP3-32201) at 1/1,000 dilution.<br/><br/> Lane 1: Jurkat cell lysate<br/> Lane 2: Daudi cell lysate<br/> Lane 3: K562 cell lysate<br/> Lane 4: HL-60 cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 49 kDa<br/> Observed band size: 55 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32201) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32201_rabbit-c-myc-mab-ps00-11-278202415212443.jpg "Western Blot: c-Myc Antibody (PS00-11) [NBP3-32201] - Western blot analysis of c-Myc on different lysates with Rabbit anti-c-Myc antibody (NBP3-32201) at 1/1,000 dilution.<br/><br/> Lane 1: Jurkat cell lysate<br/> Lane 2: Daudi cell lysate<br/> Lane 3: K562 cell lysate<br/> Lane 4: HL-60 cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 49 kDa<br/> Observed band size: 55 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32201) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: c-Myc Antibody (PS00-11) [NBP3-32201] - Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-c-Myc antibody (NBP3-32201) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32201) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32201_rabbit-c-myc-mab-ps00-11-298202413585552.jpg)
![Flow Cytometry: CD20 Antibody (10F381 (rituximab)) - Chimeric [NBP2-52646] - Flow-cytometry using the anti-CD20 research biosimilar antibody Rituximab. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Rituximab (NBP2-52646, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD20-Antibody-10F381-rituximab-Chimeric-Flow-Cytometry-NBP2-52646-img0003.jpg "Flow Cytometry: CD20 Antibody (10F381 (rituximab)) - Chimeric [NBP2-52646] - Flow-cytometry using the anti-CD20 research biosimilar antibody Rituximab. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Rituximab (NBP2-52646, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD20 Antibody (10F381 (rituximab)) - Chimeric [NBP2-52646] - Flow-cytometry using the anti-CD20 research biosimilar antibody Rituximab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Rituximab (NBP2-52646, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD20-Antibody-10F381-rituximab-Chimeric-Flow-Cytometry-NBP2-52646-img0001.jpg)
![Flow Cytometry: CD20 Antibody (10F381 (rituximab)) - Chimeric [NBP2-52646] - Flow-cytometry using the anti-CD20 research biosimilar antibody Rituximab. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Rituximab (NBP2-52646, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD20-Antibody-10F381-rituximab-Chimeric-Flow-Cytometry-NBP2-52646-img0002.jpg)
![Western Blot: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - HepG2 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa, though this protein is also glycosylated (Uniprot ID: P02771). NBP2-82221 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AF5-Western-Blot-NBP2-82221-img0002.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - HepG2 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa, though this protein is also glycosylated (Uniprot ID: P02771). NBP2-82221 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.")
![Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-82221, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AF5-Immunocytochemistry-Immunofluorescence-NBP2-82221-img0001.jpg)
![Flow Cytometry: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - HepG2 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of ([NBP2-82221], blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AF5-Flow-Cytometry-NBP2-82221-img0003.jpg)
![Adhesion Activation: HGF R/c-MET Antibody (8) [NBP2-50172] - Epitope position on molecule](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-8-Adhesion-Activation-NBP2-50172-img0001.jpg "Adhesion Activation: HGF R/c-MET Antibody (8) [NBP2-50172] - Epitope position on molecule")
![Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/1497R) [NBP2-49872] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Antibody (IGEL/1497R).](http://images.novusbio.com/fullsize/CD20-Antibody-IGEL-1497R-Immunohistochemistry-Paraffin-NBP2-49872-img0003.jpg "Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/1497R) [NBP2-49872] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Antibody (IGEL/1497R).")
![Flow Cytometry: CD20 Antibody (IGEL/1497R) [NBP2-49872] - Flow Cytometric Analysis of Raji cells. CD20 Antibody (IGEL/1497R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD20-Antibody-IGEL-1497R-Flow-Cytometry-NBP2-49872-img0002.jpg)