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![Western Blot: S100B Antibody (SPM354) [NBP2-53271] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Western-Blot-NBP2-53271-img0002.jpg "Western Blot: S100B Antibody (SPM354) [NBP2-53271] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).")
![Immunohistochemistry-Paraffin: S100B Antibody (SPM354) [NBP2-53271] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Immunohistochemistry-Paraffin-NBP2-53271-img0001.jpg)
![Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] - (1ug/ml) staining of Kelly (A) and K562 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1529_goat-polyclonal-serpin-i1-neuroserpin-antibody-129202311344711.jpg "Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] - (1ug/ml) staining of Kelly (A) and K562 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Serpin I1/Neuroserpin Antibody [NB100-1529] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-1529_goat-polyclonal-serpin-i1-neuroserpin-antibody-12920231134471.jpg)
![Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] - (1ug/ml) staining of Human Placenta (A), Mouse Brain (B) and (0.5ug/ml) Rat Braun (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1529_goat-polyclonal-serpin-i1-neuroserpin-antibody-12920231134479.jpg)
![Immunohistochemistry-Paraffin: Serpin I1/Neuroserpin Antibody [NB100-1529] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nb100-1529_goat-polyclonal-serpin-i1-neuroserpin-antibody-12920231134477.jpg)
![Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - Effect of TBeta4 on primary cilia formation in HeLa cells.The mRNA (upper) and protein (lower) expression of TBeta4 were shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-43235-1) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Thymosin-beta-4-Antibody-Western-Blot-H00007114-B01P-img0005.jpg "Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - Effect of TBeta4 on primary cilia formation in HeLa cells.The mRNA (upper) and protein (lower) expression of TBeta4 were shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-43235-1) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Thymosin beta 4 Antibody [H00007114-B01P] - Analysis of purified antibody to TMSB4X on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Thymosin-beta-4-Antibody-Immunocytochemistry-Immunofluorescence-H00007114-B01P-img0003.jpg)
![Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - Analysis of TMSB4X expression in transfected 293T cell line by TMSB4X polyclonal antibody. Lane 1: TMSB4X transfected lysate(4.84 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Thymosin-beta-4-Antibody-Western-Blot-H00007114-B01P-img0004.jpg)
![Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - No effect of T beta 4 on upstream-deleted mutant of NPHP3-promoter (pmt.). (a) Mutant (mt.) NPHP3-promoter plasmids were prepared from wildtype (wt.) promoter. (b–e) HeLa cells were transfected with pCMV-2B or pCMV-T beta 4 for 24 h. (b) HeLa cells were co-transfected with wildtype or mutant pEZX-PG02-NPHP3-promoter Gluc plasmid. & Gluc activity in cultured media was measured with luminometer using Gluc substrate. Bar graph indicates the mean of NPHP3-promoter activity. (c) Expression level of T beta 4 & NPHP3 transcripts were measured by RT-PCR. (d) The cells were fixed & stained with antibody against Ac-tubulin (red) & DAPI (blue). (e) The ciliated cells in pCMV-2B- (white) or pCMV-T beta 4-transfected group (grey) were counted. (f) Mutant (mt.) T beta 4-promoter plasmids were prepared from wildtype (wt.) promoter. (g,h) HeLa cells were transfected with pCDNA3.1 or pCDNA6-NPH3 for 24 h. (g) HeLa cells were co-transfected with wildtype or mutant pEZX-PG02-T beta 4-promoter Gluc plasmid. & Gluc activity in cultured media was measured with luminometer using Gluc substrate. Bar graph indicates the mean of T beta 4-promoter activity. (h) Expression level of T beta 4 & NPHP3 transcripts were measured by RT-PCR. Processing (such as changing brightness & contrast) is applied equally to controls across the entire image (c,d & h). Data in a bar graph represent the means ± SEM. **p 500 cells). (e,f) Cells were transfected with pEGFP-2B or pEGFP-T beta 4 plasmid for 24 h. (e) The expression of GFP & T beta 4-GFP were detected with GFP antibody. (f) The cells were fixed & stained with antibody against Ac-tubulin (red) & DAPI (blue). The ciliated cells in GFP (white) or T beta 4-GFP-positive cells (grey) were counted. Processing (such as changing brightness & contrast) is applied equally to controls across the entire image. Data in a bar graph represent the means ± SEM. **p < 0.01; significantly different from control cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31048733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007114-b01p_mouse-polyclonal-thymosin-beta-4-antibody-310202415483649.jpg)
![Western Blot: TNF RI/TNFRSF1A Antibody [NBP2-78785] - Total protein from human and mouse liver and placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNFR1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Western-Blot-NBP2-78785-img0002.jpg "Western Blot: TNF RI/TNFRSF1A Antibody [NBP2-78785] - Total protein from human and mouse liver and placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNFR1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: TNF RI/TNFRSF1A Antibody [NBP2-78785] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of TNFRI antibody (NBP2-78785). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly moderately strong reactivity in glandular cell cytoplasm.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Immunohistochemistry-Paraffin-NBP2-78785-img0001.jpg)
![Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-78132-img0002.jpg "Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-78132] - IHC analysis of a formalin fixed paraffin embedded tissue section of human appendix using 1:200 dilution of IL-6 antibody (NBP2-78132). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated membrane staining of glandular cells.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunohistochemistry-Paraffin-NBP2-78132-img0001.jpg)
![Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249415975.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - CYR61 antibody [HL2144] detects CYR61 protein at secreted protein by immunofluorescent analysis. Sample: C2C12 cells were fixed in ice-cold MeOH for 5 min. Green: CYR61 stained by CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-212024941486.jpg)
![Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249351373.jpg)
![Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - CYR61 antibody [HL2144] detects CYR61 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CYR61 stained by CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249342777.jpg)
![Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249383159.jpg)
![Western Blot: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25383_rabbit-bcl-xl-mab-hl2038-azide-and-bsa-free-2120249413345.jpg "Western Blot: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25383_rabbit-bcl-xl-mab-hl2038-azide-and-bsa-free-212024940819.jpg)
![Immunohistochemistry-Paraffin: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Bcl-XL antibody [HL2038] detects Bcl-XL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat intestine. Bcl-XL stained by Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25383_rabbit-bcl-xl-mab-hl2038-azide-and-bsa-free-212024939114.jpg)
![Western Blot: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25383_rabbit-bcl-xl-mab-hl2038-azide-and-bsa-free-2120249425465.jpg)
![Immunohistochemistry-Paraffin: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Bcl-XL antibody [HL2038] detects Bcl-XL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Bcl-XL stained by Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25383_rabbit-bcl-xl-mab-hl2038-azide-and-bsa-free-212024941414.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0003.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0001.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon cancer. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0002.jpg)
![Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - MCF7, Antibody Dilution: 1.0 ug/ml UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in MCF7.](http://images.novusbio.com/fullsize/UbcH5c-UBE2D3-Antibody-Western-Blot-NBP1-55276-img0007.jpg "Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - MCF7, Antibody Dilution: 1.0 ug/ml UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in MCF7.")
![Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/UbcH5c-UBE2D3-Antibody-Western-Blot-NBP1-55276-img0002.jpg)
![Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.](http://images.novusbio.com/fullsize/UbcH5c-UBE2D3-Antibody-Western-Blot-NBP1-55276-img0003.jpg)
![Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Human Fetal Liver, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/UbcH5c-UBE2D3-Antibody-Western-Blot-NBP1-55276-img0004.jpg)
![Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/UbcH5c-UBE2D3-Antibody-Western-Blot-NBP1-55276-img0005.jpg)
![Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Jurkat, Antibody Dilution: 1.0 ug/ml UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.](http://images.novusbio.com/fullsize/UbcH5c-UBE2D3-Antibody-Western-Blot-NBP1-55276-img0006.jpg)
![Western Blot: UbcH5c/UBE2D3 Antibody [NBP1-55276] - Lanes: 1 : 40 ng HIS-UBE2D1 protein2: 40 ng HIS-UBE2D2 protein3: 40 ng HIS-UBE2D3 protein4: 40 ng HIS-UBE2D4 protein5: 40 ng HIS-UBE2E1 protein6: 40 ng HIS-UBE2E2 protein7: 40 ng HIS-UBE2E3 protein8: 40 ng HIS-UBE2K protein9: 40 ng HIS-UBE2L3 protein10: 40 ng HIS-UBE2N protein11 : 40 ng HIS-UBE2V1 protein12: 40 ng HIS-UBE2V2 protein. Primary Antibody Dilution: 1 : 500 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 50,000.](http://images.novusbio.com/fullsize/UbcH5c-UBE2D3-Antibody-Western-Blot-NBP1-55276-img0008.jpg)