| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37439-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52495-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05179-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35153-1mg | |||
| Carrier-Free | NBP2-35153-100ug | |||
| Carrier-Free | NBP2-35153-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75901SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20107-0.025mg | |||
![Western Blot: Uteroglobin/SCGB1A1 Antibody (3A8B8) [NBP2-37439] - Analysis using SCGB1A1 mAb against HEK293 (1) and SCGB1A1 (AA: 26-91)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Uteroglobin-SCGB1A1-Antibody-3A8B8-Western-Blot-NBP2-37439-img0003.jpg "Western Blot: Uteroglobin/SCGB1A1 Antibody (3A8B8) [NBP2-37439] - Analysis using SCGB1A1 mAb against HEK293 (1) and SCGB1A1 (AA: 26-91)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: Uteroglobin/SCGB1A1 Antibody (3A8B8) [NBP2-37439] - Analysis of A549 cells using SCGB1A1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Uteroglobin-SCGB1A1-Antibody-3A8B8-Flow-Cytometry-NBP2-37439-img0004.jpg)
![Western Blot: Uteroglobin/SCGB1A1 Antibody (3A8B8) [NBP2-37439] - Analysis using SCGB1A1 mAb against human SCGB1A1 recombinant protein. (Expected MW is 33.4 kDa)](http://images.novusbio.com/fullsize/Uteroglobin-SCGB1A1-Antibody-3A8B8-Western-Blot-NBP2-37439-img0002.jpg)
![ELISA: Uteroglobin/SCGB1A1 Antibody (3A8B8) [NBP2-37439] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Uteroglobin-SCGB1A1-Antibody-3A8B8-ELISA-NBP2-37439-img0001.jpg)
![Western Blot: IL-28A/IFN-lambda 2 Antibody (6H9E6) [NBP2-52495] - Analysis using IL28A mAb against human IL28A (AA: 1-200) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-6H9E6-Western-Blot-NBP2-52495-img0006.jpg "Western Blot: IL-28A/IFN-lambda 2 Antibody (6H9E6) [NBP2-52495] - Analysis using IL28A mAb against human IL28A (AA: 1-200) recombinant protein. (Expected MW is 48.2 kDa)")
![Immunocytochemistry/Immunofluorescence: IL-28A/IFN-lambda 2 Antibody (6H9E6) [NBP2-52495] - Analysis of Hela cells using IL28A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-6H9E6-Immunocytochemistry-Immunofluorescence-NBP2-52495-img0004.jpg)
![Western Blot: IL-28A/IFN-lambda 2 Antibody (6H9E6) [NBP2-52495] - Analysis using IL28A mAb against HEK293 (1) and IL28A (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-6H9E6-Western-Blot-NBP2-52495-img0007.jpg)
![ELISA: IL-28A/IFN-lambda 2 Antibody (6H9E6) [NBP2-52495] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-6H9E6-ELISA-NBP2-52495-img0001.jpg)
![ELISA: Exendin-4 Antibody (35) - BSA Free [NBP1-05179] - Analysis using Biotin conjugate of NBP1-05179. NBP1-05180 is used as capture antibody in combination with a biotinylated detection antibody from the NBP2-23578 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated NBP1-05179 as the detection antibody.](http://images.novusbio.com/fullsize/Exendin-4-Antibody-35-BSA-Free-ELISA-NBP1-05179-img0001.jpg "ELISA: Exendin-4 Antibody (35) - BSA Free [NBP1-05179] - Analysis using Biotin conjugate of NBP1-05179. NBP1-05180 is used as capture antibody in combination with a biotinylated detection antibody from the NBP2-23578 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated NBP1-05179 as the detection antibody.")
![ELISA: Mouse Defensin beta 2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-33158] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-33158_mouse-defensin-beta-2-ready-to-use-elisa-kit-color-14820241635416.png "ELISA: Mouse Defensin beta 2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-33158] - Standard Curve Reference")
![Western Blot: TFF3 Antibody (JE32-01) [NBP3-33047] - Western blot analysis of TFF3 on human small intestine tissue lysates with Rabbit anti-TFF3 antibody (NBP3-33047) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 8 kDa Observed band size: 11 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33047) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33047_rabbit-tff3-mab-je32-01-288202412493429.jpg "Western Blot: TFF3 Antibody (JE32-01) [NBP3-33047] - Western blot analysis of TFF3 on human small intestine tissue lysates with Rabbit anti-TFF3 antibody (NBP3-33047) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 8 kDa Observed band size: 11 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33047) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TFF3 Antibody (JE32-01) [NBP3-33047] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-TFF3 antibody (NBP3-33047) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33047) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33047_rabbit-tff3-mab-je32-01-29820241492632.jpg)
![Flow Cytometry: TFF3 Antibody (JE32-01) [NBP3-33047] - Flow cytometric analysis of HT-29 cells labeling TFF3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33047, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33047_rabbit-tff3-mab-je32-01-29202416372160.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![Western Blot: CXCL13/BLC/BCA-1 Antibody (PSH04-34) [NBP3-32234] - Western blot analysis of CXCL13/BLC/BCA-1 on different lysates with Rabbit anti-CXCL13/BLC/BCA-1 antibody (NBP3-32234) at 1/1,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-CXCL13 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 13 kDa Observed band size: 12/13 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32234) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32234_rabbit-cxcl13-blc-bca-1-mab-psh04-34-27820241631655.jpg "Western Blot: CXCL13/BLC/BCA-1 Antibody (PSH04-34) [NBP3-32234] - Western blot analysis of CXCL13/BLC/BCA-1 on different lysates with Rabbit anti-CXCL13/BLC/BCA-1 antibody (NBP3-32234) at 1/1,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-CXCL13 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 13 kDa Observed band size: 12/13 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32234) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CXCL13/BLC/BCA-1 Antibody (PSH04-34) [NBP3-32234] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CXCL13/BLC/BCA-1 antibody (NBP3-32234) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32234) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32234_rabbit-cxcl13-blc-bca-1-mab-psh04-34-298202414333650.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCL13/BLC/BCA-1 Antibody (PSH04-34) [NBP3-32234] - Immunocytochemistry analysis of HeLa transfacted with or without CXCL13/BLC/BCA-1 cells labeling CXCL13/BLC/BCA-1 with Rabbit anti-CXCL13/BLC/BCA-1/BLC/BCA-1 antibody (NBP3-32234) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL13 antibody (NBP3-32234) at 1/400 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32234_rabbit-cxcl13-blc-bca-1-mab-psh04-34-29202410334051.jpg)
![Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-278202415212474.jpg "Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32072) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-298202414333630.jpg)
![Flow Cytometry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Flow cytometric analysis of THP-1 cells labeling Bcl-2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32072, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-29202416372125.jpg)
![In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-In-vitro-assay-NBP3-14783-img0001.jpg "In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence")
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant Human SOD1-Cu-Zn SOD Pre-formed Fibrils Protein-Electron Microscopy-NBP3-14783-img0003.jpg)
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP3-14783-img0002.jpg)
![SDS-Page: Mouse LIF Protein [NBP2-35153]](http://images.novusbio.com/fullsize/Mouse-LIF-Protein-SDS-Page-NBP2-35153-img0001.jpg "SDS-Page: Mouse LIF Protein [NBP2-35153]")
![Western Blot: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-75901] - Mouse lipocyte (lane 1), mouse spleen (lane 2), mouse thymus (lane 3), mouse lymph node (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75901 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Daclizumab-Western-Blot-NBP2-75901-img0002.jpg "Western Blot: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-75901] - Mouse lipocyte (lane 1), mouse spleen (lane 2), mouse thymus (lane 3), mouse lymph node (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75901 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.")
![Immunohistochemistry: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-75901] - Formalin fixed rat spleen slices were were stained with NBP2-75901 at 5 ug/ml.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Daclizumab-Immunohistochemistry-NBP2-75901-img0001.jpg)
![Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924254.png "Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (NBP3-20107, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023964610.png)
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41A-1 (NBP3-20107) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20107, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924231.png)