| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90052-25ul | |||
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD21 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Mouse Anti-Human CD21, Monoclonal Antibody (Catalog # NBP3-26866) at 1:800 at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26866-25ul | |||
| Azide and BSA Free | NBP3-44288 | |||
![Mouse IgM Isotype Control (PFR-03) [NBP2-62224] - Example of nonspecific mouse IgM (PFR-03; purified, GAM-APC) signal on human peripheral blood; surface staining, 6 ug/ml.](http://images.novusbio.com/fullsize/Mouse-IgM-Isotype-Control-PFR-03-NBP2-62224-img0008.jpg "Mouse IgM Isotype Control (PFR-03) [NBP2-62224] - Example of nonspecific mouse IgM (PFR-03; purified, GAM-APC) signal on human peripheral blood; surface staining, 6 ug/ml.")
![Mouse IgM Isotype Control (PFR-03) [NBP2-62224] - Example of nonspecific mouse IgM (PFR-03) FITC signal on human peripheral blood; surface staining, 1 ug/ml.](http://images.novusbio.com/fullsize/Mouse-IgM-Isotype-Control-PFR-03-NBP2-62224-img0009.jpg)
![Mouse IgM Isotype Control (PFR-03) [NBP2-62224] - Example of nonspecific mouse IgM (PFR-03) PE signal on human peripheral blood; surface staining, 3 ug/ml.](http://images.novusbio.com/fullsize/Mouse-IgM-Isotype-Control-PFR-03-NBP2-62224-img0010.jpg)
![Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 34 kDa<br/> Observed band size: 45 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-278202416285274.jpg "Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 34 kDa<br/> Observed band size: 45 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD38 Antibody (PD01-49) [NBP3-32151] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD38 antibody (NBP3-32151) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32151) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29820241435231.jpg)
![Immunocytochemistry/ Immunofluorescence: CD38 Antibody (PD01-49) [NBP3-32151] - Immunocytochemistry analysis of A549 cells labeling CD38 with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-2920241192330.jpg)
![Flow Cytometry: CD38 Antibody (PD01-49) [NBP3-32151] - Flow cytometric analysis of THP-1 cells labeling CD38.<br/><br/> Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32151, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29202416453252.jpg)
![Western blot analysis on different lysates with NBP3-32151 at 1/2,000 dilution. Lane 1: Daudi cell lysate Lane 2: Ramos cell lysate Lane 3: HepG2 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32151 at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32151_-western-blot-639204594552926229.jpg)
![Western blot analysis on different lysates with NBP3-32465 at 1/1,000 dilution. Lane 1: THP-1 cell lysate (30 µg/Lane) Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate (30 µg/Lane) Lane 3: Mouse spleen tissue lysate (40 µg/Lane) Lane 4: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minutes 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32465 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32465_-western-blot-639204597483225525.jpg)
![Flow Cytometry: Syndecan-1/CD138 Antibody (B-A38) [NBP3-14568] - A typical staining pattern with the B-A38 monoclonal antibody of U266 cell line.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-B-A38-Flow-Cytometry-NBP3-14568-img0001.jpg "Flow Cytometry: Syndecan-1/CD138 Antibody (B-A38) [NBP3-14568] - A typical staining pattern with the B-A38 monoclonal antibody of U266 cell line.")
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90052] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90052-img0005.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90052] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90052-img0004.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90052] - Staining of human cerebral cortex using Anti-MS4A1 antibody NBP1-90052.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90052-img0012.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90052] - Staining of human lymph node.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90052-img0008.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90052] - Staining of human spleen.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90052-img0009.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90052] - Staining of human testis.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90052-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CD20 Antibody [NBP1-90052] - Analysis using Anti-MS4A1 antibody NBP1-90052 (A) shows similar pattern to independent antibody NBP1-90051 (B).](http://images.novusbio.com/fullsize/CD20-Antibody-Western-Blot-NBP1-90052-img0011.jpg)
![Detection of human CD38 by western blot.<br/><br/>Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, MOLT-4, RPMI-8226, CCRF-CEM, A-549, Ramos, Raji, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] (NBP3-14707 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/nbp3-14707_-western-blot-639168047996473616.jpg "Detection of human CD38 by western blot.<br/><br/>Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, MOLT-4, RPMI-8226, CCRF-CEM, A-549, Ramos, Raji, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] (NBP3-14707 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![Detection of human CD38 by western blot of immunoprecipitates.<br/><br/>Samples: Whole cell lysate (1.0 mg per IP reaction; 2% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] (NBP3-14707 lot 1) used for IP at 20 ul/mg lysate. CD38 was also immunoprecipitated by rabbit anti-CD38 antibody BL1874B-2A3. For blotting immunoprecipitated CD38, NBP3-14707 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/nbp3-14707_-western-blot-639168073921995763.jpg)
![Detection of human CD38 in FFPE colon carcinoma by immunohistochemistry.<br/><br/>Antibody: Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] (NBP3-14707 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14707_-immunohistochemistry-639168047996844214.jpg)
![Detection of human CD38 in FFPE breast carcinoma by immunohistochemistry.<br/><br/>Antibody: Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] (NBP3-14707 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14707_-immunohistochemistry-639168047996776841.jpg)
![Detection of human CD38 in FFPE tonsil by immunohistochemistry.<br/><br/>Antibody: Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] NBP3-14707 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14707_-immunohistochemistry-639168047997607618.jpg)
![Detection of human CD38 in FFPE CCRF-CEM cells by immunocytochemistry.<br/><br/>Antibody: Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] (NBP3-14707 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14707_-immunocytochemistry-immunofluorescence-639168047997056254.jpg)
![Detection of human CD38 (shaded) in Ramos cells by flow cytometry.<br/><br/>Antibody: Rabbit anti-CD38 recombinant monoclonal [BLR123H] (NBP3-14707) or isotype control (unshaded). Secondary: DyLight(R) 488-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp3-14707_-flow-cytometry-639168047997288504.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-western-blot-1311202411142..png "Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.")
![Flow Cytometry: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Antibody: Rabbit anti-CD86 recombinantmonoclonal [BLR030F] or isotype control(unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-flow-cytometry-1311202411333..png)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -FFPE section of tonsil. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunohistochemistry-paraffin-131120241151..png)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -Section of HDLM-2 cells. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunocytochemistry-immunofluorescence-1311202411712..png)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-1312025145029..jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417) and rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416).](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-131202515750..jpg)
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) biotin, streptavidin.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0012.jpg "Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) biotin, streptavidin.")
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0001.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) FITC.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0002.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM7.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0003.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM5.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0004.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Alexa Fluor(R) 700.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0005.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC-CyTM7.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0006.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0007.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0008.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE-DyLigt(R) 594.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0009.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0010.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0011.jpg)
![Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP2-60672-img0011.jpg "Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Image using the Biotin form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc). Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60672-img0007.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0008.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0012.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat anti-Rat IgM Heavy Chain Secondary Antibody-Fluorophore-linked immunosorbent assay-NBP2-60672-img0014.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0013.jpg)
