| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26152-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47668-0.1mg | |||
| IHC-Prediluted | NBP2-48291 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48090 | |||
| Azide and BSA Free | NBP2-86683 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45143 | |||
| Azide and BSA Free | NBP2-34691-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47739-0.1mg | |||
![Immunoprecipitation: MEK1 Antibody (3F10) [NBP3-26152] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26152 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26152 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26152_rabbit-mek1-mab-3f10-2622024132373.jpg "Immunoprecipitation: MEK1 Antibody (3F10) [NBP3-26152] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26152 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26152 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: MEK1 Antibody (3F10) [NBP3-26152] - Image of MEK1 Antibody (3F10) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26152_rabbit-mek1-mab-3f10-27220248573314.jpg)
![Western Blot: MEK1 Antibody (3F10) [NBP3-26152] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate. All lanes: MEK1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 44, 41 kDa Observed band size: 44 kDa](http://images.novusbio.com/fullsize/nbp3-26152_rabbit-mek1-mab-3f10-2822024114090.jpg)
![Western Blot: TFEB Antibody [NBP2-12758] - Detection of Human TFEB by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 10 second.](http://images.novusbio.com/fullsize/TFEB-Antibody-Western-Blot-NBP2-12758-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: TFEB Antibody [NBP2-12758] - Detection of Human TFEB by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 10 second.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: TFEB Antibody [NBP2-12758] - Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for IP at 6 ug per reaction. TFEB was also immunoprecipitated by a different rabbit anti-TFEB antibody and rabbit anti-TFEB recombinant monoclonal antibody (NBP2-77360). For blotting immunoprecipitated TFEB, NBP2-77360 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/TFEB-Antibody-Immunoprecipitation-NBP2-12758-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (SPM145) [NBP2-45297] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM145).](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM145-Immunohistochemistry-Paraffin-NBP2-45297-img0001.jpg "Immunohistochemistry-Paraffin: NF-H Antibody (SPM145) [NBP2-45297] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM145).")
![Immunohistochemistry-Paraffin: c-Myc Antibody (MYC909) [NBP2-45146] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (MYC909).](http://images.novusbio.com/fullsize/c-Myc-Antibody-MYC909-Immunohistochemistry-Paraffin-NBP2-45146-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (MYC909) [NBP2-45146] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (MYC909).")
![Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast tissue. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used at at dilution of 1:250 and incubated for 18 hours at room temperature. Detection: DAB](http://images.novusbio.com/fullsize/PCNA-[p-Tyr211]-Antibody-Immunohistochemistry-NB100-59861-img0005.jpg "Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast tissue. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used at at dilution of 1:250 and incubated for 18 hours at room temperature. Detection: DAB")
![Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used a dilution of 1:250 and incubated for 18 hours at room temeprature. Detection: DAB](http://images.novusbio.com/fullsize/PCNA-[p-Tyr211]-Antibody-Immunohistochemistry-NB100-59861-img0004.jpg)
![Western Blot: VPS4B Antibody [NBP1-19119] - Whole cell lysate from HeLa and 293T cells. VPS4B was also immunoprecipitated by rabbit anti-VPS4B antibody NBP1-19118.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Western-Blot-NBP1-19119-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: VPS4B Antibody [NBP1-19119] - Whole cell lysate from HeLa and 293T cells. VPS4B was also immunoprecipitated by rabbit anti-VPS4B antibody NBP1-19118.")
![Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody NB100-766 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NB100-766-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody NB100-766 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: RPTOR Antibody [NB100-766] - Detection of human Raptor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Raptor antibody NB100-766 (lot 3) used for IP at 6 ug per reaction. Raptor was also immunoprecipitated by a previous lot of this antibody (lot 2) and rabbit anti-Raptor antibody NB100-331 For blotting immunoprecipitated Raptor, NB100-766 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunoprecipitation-NB100-766-img0005.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (MYC275) [NBP2-32839] - Formalin-fixed, paraffin-embedded human Cerivcal carcinoma stained with c-Myc Monoclonal Antibody (MYC275).](http://images.novusbio.com/fullsize/c-Myc-Antibody-MYC275-Immunohistochemistry-Paraffin-NBP2-32839-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (MYC275) [NBP2-32839] - Formalin-fixed, paraffin-embedded human Cerivcal carcinoma stained with c-Myc Monoclonal Antibody (MYC275).")
![Western Blot: PI 3-Kinase p85 beta Antibody [NBP1-41066] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-PIK3R2 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. PIK3R2 was also immunoprecipitated by rabbit anti-PIK3R2 antibody NBP1-41067, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-beta-Antibody-Western-Blot-NBP1-41066-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PI 3-Kinase p85 beta Antibody [NBP1-41066] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-PIK3R2 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. PIK3R2 was also immunoprecipitated by rabbit anti-PIK3R2 antibody NBP1-41067, which recognizes a downstream epitope.")
![Immunohistochemistry-Paraffin: c-Myc Antibody (MYC275 + MYC909) [NBP2-45147] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (MYC275 + MYC909).](http://images.novusbio.com/fullsize/c-Myc-Antibody-MYC275-+-MYC909-Immunohistochemistry-Paraffin-NBP2-45147-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (MYC275 + MYC909) [NBP2-45147] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (MYC275 + MYC909).")