| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72977 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70380 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89844-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60982SS | |||
![Western Blot: NF-M Antibody (2G3) [NBP2-46139] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NF-M.](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Western-Blot-NBP2-46139-img0010.jpg "Western Blot: NF-M Antibody (2G3) [NBP2-46139] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NF-M.")
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human testicular cancer tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0009.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0006.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0007.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0008.jpg)
![NF-M Antibody (OTI2G3)[NBP2-46139]Simple Western� analysis of lysates (0.2 mg/mL) from HEK293T cell culture using CD63 mouse mAb (SKU: TA803474). EVs were harvested from cell culture media and enriched by polymer-based precipitation. EV contained cell culture media without enrichment was used as controls. The virtual lane view (left) shows the target band corresponding to CD63 (highly glycosylated) at 1:20 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary of EV lysate (Green), cell lysate (Blue) and unenriched media (Grey). The experiment was performed on the Jess Simple Western instrument for ProteinSimple, a BioTechne band, using the 12-230 kDa separation module. *CD63 is highly glycosylated protein, verified by overexpressed lysate tagged with DDK. PNGase F treatment can bring the band to the expected size.](http://images.novusbio.com/fullsize/nbp2-46139_mouse-nf-m-mab-oti2g3-simple-western-131120251236356.jpg%253fstore=america&image-type=image)
![Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167] - CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0018.jpg "Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167] - CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167] - CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0021.jpg)
![Western Blot: CD63 Antibody (5E5) [NBP2-46167] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD63 .](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Western-Blot-NBP2-46167-img0024.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human tonsil within the normal limits using CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0023.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0001.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0002.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0003.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0004.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0005.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human liver tissue.. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0006.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0007.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0008.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0009.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0010.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0011.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0012.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0013.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0014.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0015.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0016.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0017.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0019.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0020.jpg)
![Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD63-Antibody-5E5-Immunohistochemistry-NBP2-46167-img0022.jpg)
![CD63 Antibody (OTI5E5)[NBP2-46167]Simple Western� analysis of endogenous protein OTC from HELA lysates (0.2 mg/mL) using OTC Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-46167_mouse-cd63-mab-oti5e5-simple-western-1311202512362217.jpg%253fstore=america&image-type=image)
![Western Blot: TSC1 Antibody [NB100-2821] - Staining of Human Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TSC1-Antibody-Western-Blot-NB100-2821-img0001.jpg "Western Blot: TSC1 Antibody [NB100-2821] - Staining of Human Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-2821] - Staining of paraffin embedded Human Liver. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TSC1-Antibody-Immunohistochemistry-Paraffin-NB100-2821-img0005.jpg)
![Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-2821] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TSC1-Antibody-Immunohistochemistry-Paraffin-NB100-2821-img0002.jpg)
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0005.jpg "Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of various cell lines, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts from normal (control) and PTEN knockout (KO) HeLa cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Knockout-Validated-NBP3-15666-img0001.jpg)
![Immunoprecipitation: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of 300ug extracts of NIH/3T3 cells using 3ug PTEN antibody (NBP3-15666). Western blot was performed from the immunoprecipitate using PTEN antibody (NBP3-15666) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Immunoprecipitation-NBP3-15666-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: PTEN Antibody (7O6F4) [PTEN] - Confocal imaging of NIH/3T3 cells using [KO Validated] PTEN Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15666_rabbit-pten-mab-7o6f4-52202517361229.jpg)
![SDS-Page: Recombinant Human GAPDH Protein [NBP2-52615] - Observed molecular weight: 36 kDa](http://images.novusbio.com/fullsize/Recombinant-Human-GAPDH-Protein-SDS-Page-NBP2-52615-img0002.jpg "SDS-Page: Recombinant Human GAPDH Protein [NBP2-52615] - Observed molecular weight: 36 kDa")
![Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Western-Blot-NBP3-15310-img0003.jpg "Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD133 Rabbit mAb (NBP3-15310) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Immunohistochemistry-Paraffin-NBP3-15310-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts from normal (control) and CD133 knockout (KO) HCT116 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Knockout-Validated-NBP3-15310-img0001.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [CD133] - Western blot analysis of lysates from HT-29 cells, using CD133 Rabbit mAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517192943.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-522025172608.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517251995.jpg)
![Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-644-img0002.jpg "Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.")
![Simple Western: PINK1 Antibody [NB100-644] - Simple Western lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~56 kDa. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NB100-644-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0002.jpg "Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000).")
![<b>Biological Strategies Validation. </b>Flow Cytometry: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1(CD107a) on Jurkat cells. Cells were treated, stained with purified anti-Human LAMP1(CD107a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Flow-Cytometry-NBP2-89844-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human placenta with rabbit monoclonal antibody (1:1000). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0001.jpg)
![LAMP-1/CD107a was immunoprecipitated using NBP2-89844 at a 1:100 dilution and 60ug Immunomagnetic beads Protein A/G and Goat Anti-Rabbit IgG (H+L)[HRP] as a secondary antibody at 1/10000 dilution: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg Daudi Whole Cell Lysate. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 120 kDa](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-4520231646141.png)
![<b>Genetic Strategies Validation. </b>Western Blot: LAMP-1/CD107a Antibody (107) [NBP2-89844] - NBP2-89844 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: LAMP-1/CD107a knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 120 kDa. Observed band size: 100-130 kDa](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-136202422345046.png)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89844 antibody (1:300). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor(R) 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-235202514492341.png)
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0012.jpg "Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (11H10) [NBP2-78827] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Tissue: Intestinal Cancer. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunohistochemistry-NBP2-78827-img0013.jpg)
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 11H10 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0007.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of MEF lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0010.jpg)
![Western Blot: Rictor Antibody [NB100-56427] - Analysis of Rictor in Jurkat cell lysate using this antibody.](http://images.novusbio.com/fullsize/RICTOR-Antibody-Western-Blot-NB100-56427-img0002.jpg "Western Blot: Rictor Antibody [NB100-56427] - Analysis of Rictor in Jurkat cell lysate using this antibody.")
![Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Western Blot: [NBP2-49688] - 10 ug of Chloroquine treated (+) and untreated (-) Neuro2A lysates in 1x Laemmli sample buffer (NBP2-49688) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0004.jpg)