| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21201-25ul | |||
| Azide and BSA Free | NBP3-44314 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21480-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56482SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24560SS | |||
![Immunohistochemistry-Paraffin: Rictor Antibody [NB100-1534] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-Paraffin-NB100-1534-img0002.jpg "Immunohistochemistry-Paraffin: Rictor Antibody [NB100-1534] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry: Rictor Antibody [NB100-1534] - The combined silencing of Rictor and EGFR in vivo results in a complete inhibition of tumor growth. U251Ng2x, U251Rictor, U251EGFR and U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice. Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline and 5% sucrose in drinking water. On day 49, animals were imaged by Maestro (TM) fluorescence imaging unit expression of tRFP co-expressed with the shRNA sequences. Mice brains were harvested, sectioned and stained for nuclei, Rictor, EGFR and p(473)-AKT and imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0059597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-NB100-1534-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Rictor Antibody [NB100-1534] - Fluorescence micrographs showing EGFR (Alexa 488; green), Rictor (Alexa 488; yellow) & cell nuclei (Hoechst 33342; blue) in GBM4 GBM-derived cancer stem-like cell line, & Gli36, U251MG, U118MG & LN229 GBM cell lines. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23555046), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1534_goat-polyclonal-rictor-antibody-310202415392527.jpg)
![Immunocytochemistry/ Immunofluorescence: Rictor Antibody [NB100-1534] - The combined silencing of Rictor & EGFR in vivo results in a complete inhibition of tumor growth.U251Ng2x, U251Rictor, U251EGFR & U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice (n = 6−8). Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline & 5% sucrose in drinking water. a) On day 49, animals were imaged by Maestro™ fluorescence imaging unit for the expression of tRFP co-expressed with the shRNA sequences upon doxycycline-induced expression. Mice were then terminated & brains were harvested, sectioned & stained for nuclei, Rictor, EGFR & p(473)-AKT & imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. b) A representative brain section from U251Ng2x, U251Rictor, U251EGFR & U251EGFR/Rictor tumor groups is shown: tRFP (red) & Hoechst (blue). c) A representative tumor section from U251Ng2x, U251Rictor & U251EGFR tumor groups is shown: nuclei (blue), rRFP (red), Rictor (yellow), EGFR (green) & p(473)-AKT (orange). d) The expression of EGFR (left axis), Rictor (right axis) & p(473)-AKT (right axis) in U251Ng2x, U251Rictor, U251EGFR tumor sections were quantified (positive staining normalized to Hoechst nuclei staining). e) Tumor sizes were estimated by quantification of tumor areas in brain sections from all groups (left axis). The expression of the proliferation marker Ki67 in the tumor (proliferating fraction) was also quantified (right axis). *p-value ≤0.05; **p-value ≤0.01; ***p-value ≤0.001 compared to control untreated cells. ‡: No tumor was detected in the U251EGFR/Rictor group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23555046), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1534_goat-polyclonal-rictor-antibody-310202415525248.jpg)
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of NIH/3T3 cells using Bcl2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8E12-Immunocytochemistry-Immunofluorescence-NBP2-22170-img0002.jpg "Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of NIH/3T3 cells using Bcl2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of colon cancer tissues (left) and human brain tissues (right) using Bcl2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8E12-Immunohistochemistry-Paraffin-NBP2-22170-img0003.jpg)
![Flow Cytometry: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of 3T3L1 cells using Bcl2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8E12-Flow-Cytometry-NBP2-22170-img0001.jpg)
![Western Blot: BNIP3L Antibody (JE59-98) [NBP3-32087] - Western blot analysis of BNIP3L on different lysates with Rabbit anti-BNIP3L antibody (NBP3-32087) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 37 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32087) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32087_rabbit-bnip3l-mab-je59-98-27820241631666.jpg "Western Blot: BNIP3L Antibody (JE59-98) [NBP3-32087] - Western blot analysis of BNIP3L on different lysates with Rabbit anti-BNIP3L antibody (NBP3-32087) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 37 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32087) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: BNIP3L Antibody (JE59-98) [NBP3-32087] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-BNIP3L antibody (NBP3-32087) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32087) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32087_rabbit-bnip3l-mab-je59-98-2982024143525.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody (JE59-98) [NBP3-32087] - Immunocytochemistry analysis of HeLa cells labeling BNIP3L with Rabbit anti-BNIP3L antibody (NBP3-32087) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BNIP3L antibody (NBP3-32087) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32087_rabbit-bnip3l-mab-je59-98-292024119232.jpg)
![Flow Cytometry: BNIP3L Antibody (JE59-98) [NBP3-32087] - Flow cytometric analysis of HeLa cells labeling BNIP3L. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32087, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32087_rabbit-bnip3l-mab-je59-98-29202416453213.jpg)
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-168202375610..jpg "Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.")
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)- Human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-16820237586..jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3 treated with PDGFA 5 ug/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-168202375944.jpg)
![<b>Biological Strategies Validation. </b>Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower).](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238120.jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 ug/mL, 5 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238442..jpg)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 ug/mL, 5 min; +), using NBP3-21480 at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-136202422345072.png)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5000 ng/mL, 5min; +) (line B); treated with PDGFA and -phosphatase (line C) using NBP3-21480 at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-23520251628115.png)
![Western Blot: UVRAG Antibody [NBP2-24482] - Analysis of UVRAG in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart using this antibody at 0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.](http://images.novusbio.com/fullsize/UVRAG-Antibody-Western-Blot-NBP2-24482-img0001.jpg "Western Blot: UVRAG Antibody [NBP2-24482] - Analysis of UVRAG in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart using this antibody at 0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.")
![Western Blot: Sin1/MAPKAP1 Antibody [NB100-56482] - Analysis of Mip1 in human heart lysate in the 1) absence and 2) presence of immunizing peptide and in human skeletal muscle lysate in the 3) absence and 4) presence of immunizing peptide using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NB100-56482-img0003.jpg "Western Blot: Sin1/MAPKAP1 Antibody [NB100-56482] - Analysis of Mip1 in human heart lysate in the 1) absence and 2) presence of immunizing peptide and in human skeletal muscle lysate in the 3) absence and 4) presence of immunizing peptide using this antibody. 5 ug/ml.")
![Western Blot: MEK1 Antibody [NBP2-24542] - Analysis of MEK1/MEK2 phosphorylation in A375 whole cell lysate. Lane A shows phospho MEK1/MEK2 (upper band), and the weakly detected nonphosphorylated form (lower band), using MEK1 phospho antibody at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-Western-Blot-NBP2-24542-img0001.jpg "Western Blot: MEK1 Antibody [NBP2-24542] - Analysis of MEK1/MEK2 phosphorylation in A375 whole cell lysate. Lane A shows phospho MEK1/MEK2 (upper band), and the weakly detected nonphosphorylated form (lower band), using MEK1 phospho antibody at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.")
![Western Blot: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of neuronal tissue lysates using mouse NF-M mAb, dilution 1:10,000 (Green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145kDa correspond to rodent NF-M while that at about 160kDa corresponds to the significantly larger bovine NF-M protein.](http://images.novusbio.com/fullsize/NF-M-Antibody-MCA-3H11-Western-Blot-NBP2-31206-img0003.jpg "Western Blot: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of neuronal tissue lysates using mouse NF-M mAb, dilution 1:10,000 (Green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145kDa correspond to rodent NF-M while that at about 160kDa corresponds to the significantly larger bovine NF-M protein.")
![Immunohistochemistry Free-Floating: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of adult rat frontal cortex section stained with mouse neurofilament NF-M mAb, dilution 1:5,000 (Green), and costained with chicken neurofilament NF-H pAb, dilution 1:5,000 (Red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibodies. The mouse NF-M mAb labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the chicken NF-M pAb stains the network of neuronal axons only.](http://images.novusbio.com/fullsize/NF-M-Antibody-MCA-3H11-Immunohistochemistry-Free-Floating-NBP2-31206-img0004.jpg)
![Western Blot: Neurofilament NF-M Antibody (MCA-3H11) [NBP2-31206] - Cytoskeletal homogenate of rat spinal cord was separated by 7.5% SDS-PAGE. Lane A: Western blot analysis of Neurofilament NF-M with NBP2-31206. Lane B: SDS-PAGE gel stained with Commassie Brilliant Blue. The bands corresponding to Neurofilament subunits NF-H, NF-M, NF-L, a-internexin and GFAP are indicated.](http://images.novusbio.com/fullsize/Neurofilament-NF-M-Antibody-MCA-3H11-Western-Blot-NBP2-31206-img0002.jpg)
![Western Blot: RGS19 Antibody [NBP2-24516] - Analysis of RGS19 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24516 at 1.0 ug/ml.](http://images.novusbio.com/fullsize/RGS19-Antibody-Western-Blot-NBP2-24516-img0003.jpg "Western Blot: RGS19 Antibody [NBP2-24516] - Analysis of RGS19 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24516 at 1.0 ug/ml.")
![Immunohistochemistry-Paraffin: RGS19 Antibody [NBP2-24516] - analysis of RGS19 in paraffin-embedded formalin-fixed human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/RGS19-Antibody-Immunohistochemistry-Paraffin-NBP2-24516-img0002.jpg)
![Western Blot: MEK1 Antibody [NBP2-24560] - Analysis of MEK1/2 phosphorylation in A375 whole cell lysate. Lane A shows phosphorylated MEK1/2 (upper band), and the weakly detected nonphosphorylated form (lower band), using NBP2-24560 at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-Western-Blot-NBP2-24560-img0001.jpg "Western Blot: MEK1 Antibody [NBP2-24560] - Analysis of MEK1/2 phosphorylation in A375 whole cell lysate. Lane A shows phosphorylated MEK1/2 (upper band), and the weakly detected nonphosphorylated form (lower band), using NBP2-24560 at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.")
![Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Western-Blot-NB100-293-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")