| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-60973-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89197-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89600-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99356-50ul | |||
![Western Blot: FGFR2 Antibody [NB200-642] - Whole extract of HEK-293T cells expressing FGFR-2 were separated on SDS-PAGE and probed with Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit. The antibody was developed using 1:10,000 Goat Anti-Rabbit IgG-Peroxidase.Lanes:1. 0.5 ug/mL antibody2. 0.25 ug/mL antibody3. 0.5 ug/mL antibody untransfected 293T cells4. Negative Control](http://images.novusbio.com/fullsize/FGFR2-Antibody-Western-Blot-NB200-642-img0002.jpg "Western Blot: FGFR2 Antibody [NB200-642] - Whole extract of HEK-293T cells expressing FGFR-2 were separated on SDS-PAGE and probed with Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit. The antibody was developed using 1:10,000 Goat Anti-Rabbit IgG-Peroxidase.Lanes:1. 0.5 ug/mL antibody2. 0.25 ug/mL antibody3. 0.5 ug/mL antibody untransfected 293T cells4. Negative Control")
![Immunohistochemistry: FGFR2 Antibody [NB200-642] - Formalin fixed, paraffin-embedded Human placenta tissue sections were stained with 2 ug/mL Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit followed by 20 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 20 ug/mL ExtrAvidin-Peroxidase.](http://images.novusbio.com/fullsize/FGFR2-Antibody-Immunohistochemistry-NB200-642-img0003.jpg)
![Western Blot: FGFR2 Antibody [NB200-642] - Antibody recognition: Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Fibroblast Growth Factor Receptor-2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with 1:10,000 Goat Anti-Rabbit IgG-peroxidase and a chemiluminescent substrate.Antibody dilution: 0.5 ug/mL.](http://images.novusbio.com/fullsize/FGFR2-Antibody-Western-Blot-NB200-642-img0001.jpg)
![Immunohistochemistry-Paraffin: Laminin Antibody (4C7) [NB120-17107] - Analysis in formalin fixed paraffin embedded renal carcinoma stained with Laminin Antibody (4C7) [NB120-17107].](http://images.novusbio.com/fullsize/Laminin-Antibody-4C7-Immunohistochemistry-Paraffin-NB120-17107-img0002.jpg "Immunohistochemistry-Paraffin: Laminin Antibody (4C7) [NB120-17107] - Analysis in formalin fixed paraffin embedded renal carcinoma stained with Laminin Antibody (4C7) [NB120-17107].")
![Immunohistochemistry-Paraffin: Laminin Antibody (4C7) [NB120-17107] - Analysis in formalin fixed paraffin embedded renal carcinoma with Laminin Antibody (4C7) [NB120-17107].](http://images.novusbio.com/fullsize/Laminin-Antibody-4C7-Immunohistochemistry-Paraffin-NB120-17107-img0001.jpg)
![Immunohistochemistry-Paraffin: VEGFD Antibody (MM0007-7E79) [NB110-60973] - Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining (Proteinase K pretreatment is required).](http://images.novusbio.com/fullsize/VEGFD-Antibody-MM0007-7E79-Immunohistochemistry-Paraffin-NB110-60973-img0003.jpg "Immunohistochemistry-Paraffin: VEGFD Antibody (MM0007-7E79) [NB110-60973] - Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining (Proteinase K pretreatment is required).")
![Immunohistochemistry-Paraffin: VEGFD Antibody (MM0007-7E79) [NB110-60973] - Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using NB110-60973 (Proteinase K pretreatment is required).](http://images.novusbio.com/fullsize/VEGFD-Antibody-MM0007-7E79-Immunohistochemistry-Paraffin-NB110-60973-img0002.jpg)
![Western Blot: FGFR1OP Antibody (JE64-24) [NBP3-32339] - Western blot analysis of FGFR1OP on different lysates with Rabbit anti-FGFR1OP antibody at 1/500 dilution. Lane 1: HepG2 cell lysate, 10 ug/Lane Lane 2: Mouse kidney cell lysate, 20 ug/Lane Predicted band size: 43 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody CEP43 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32339_rabbit-fgfr1op-mab-je64-24-278202416331298.jpg "Western Blot: FGFR1OP Antibody (JE64-24) [NBP3-32339] - Western blot analysis of FGFR1OP on different lysates with Rabbit anti-FGFR1OP antibody at 1/500 dilution. Lane 1: HepG2 cell lysate, 10 ug/Lane Lane 2: Mouse kidney cell lysate, 20 ug/Lane Predicted band size: 43 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody CEP43 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FGFR1OP Antibody (JE64-24) [NBP3-32339] - Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-FGFR1OP antibody (NBP3-32339) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody CEP43 at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32339_rabbit-fgfr1op-mab-je64-24-298202414262612.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human brain with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-042-Immunohistochemistry-Paraffin-NBP2-89197-img0003.jpg "Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human brain with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).")
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human placenta with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-042-Immunohistochemistry-Paraffin-NBP2-89197-img0001.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (042) [NBP2-89197] - Staining of human NRP1 in human heart with rabbit monoclonal Neuropilin-1 antibody (042) [NBP2-89197] (1:100).](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-042-Immunohistochemistry-Paraffin-NBP2-89197-img0002.jpg)
![Western Blot: EIF3S3 Antibody (PSH04-49) [NBP3-32298] - Western blot analysis of EIF3S3 on different lysates with Mouse anti-EIF3S3 antibody (NBP3-32298) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: MDA-MB-231 cell lysate Lane 9: MDA-MB-468 cell lysate Lane 10: MCF7 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: NIH/3T3 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32298_mouse-eif3s3-mab-psh04-49-27820241631672.jpg "Western Blot: EIF3S3 Antibody (PSH04-49) [NBP3-32298] - Western blot analysis of EIF3S3 on different lysates with Mouse anti-EIF3S3 antibody (NBP3-32298) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: MDA-MB-231 cell lysate Lane 9: MDA-MB-468 cell lysate Lane 10: MCF7 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: NIH/3T3 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: EIF3S3 Antibody (PSH04-49) [NBP3-32298] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EIF3S3 antibody (NBP3-32298) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32298) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32298_mouse-eif3s3-mab-psh04-49-298202414161894.jpg)
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-1572024145956..jpg "Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE-1 antibody at 1/1,000 dilution.Lane 1: A549-si NT cell lysateLane 2: A549-si LYVE-1 cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-66940 was shown to specifically react with LYVE-1 in A549-si NT cells. Weakened band was observed when A549-si LYVE-1 sample was tested. A549-si NT and Hela-si LYVE-1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-29, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-15720241537..jpg)
![Flow Cytometry: LYVE-1 Antibody (JF0979) [NBP2-66940] - Analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/LYVE-1-Antibody-JF0979-Flow-Cytometry-NBP2-66940-img0001.jpg)
![Western Blot: Angiopoietin-2 Antibody (117) [NBP2-89600] - Lane A: HepG2 Whole Cell Lysate Lane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:70 kDa](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-117-Western-Blot-NBP2-89600-img0002.jpg "Western Blot: Angiopoietin-2 Antibody (117) [NBP2-89600] - Lane A: HepG2 Whole Cell Lysate Lane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:70 kDa")
![Immunoprecipitation: Angiopoietin-2 Antibody (117) [NBP2-89600] - Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 0.5 uL anti-Ang2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Ang2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 57 kDaObserved band size: 57 kDa](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-117-Immunoprecipitation-NBP2-89600-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Angiopoietin-2 Antibody (117) [NBP2-89600] - Staining of Human Ang2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89600 antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89600_rabbit-monoclonal-angiopoietin-2-antibody-117-235202516335034.png)
![Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-288202412493428.jpg "Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33008) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-29820241420920.jpg)
![Western Blot: FIH-1/HIF-1AN Antibody [NBP2-99356] - Anti-FIH-1/HIF-1AN rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Western-Blot-NBP2-99356-img0003.jpg "Western Blot: FIH-1/HIF-1AN Antibody [NBP2-99356] - Anti-FIH-1/HIF-1AN rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-99356] - Immunochemical staining of human FIH-1/HIF-1AN in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunohistochemistry-Paraffin-NBP2-99356-img0002.jpg)
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-99356] - Immunochemical staining of human FIH-1/HIF-1AN in human kidney with NBP2-99356 at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-99356_rabbit-polyclonal-fih-1-hif-1an-antibody-23520252135149.png)
![Immunocytochemistry/Immunofluorescence: FGF18 Antibody [NBP3-06688] - Immunofluorescence analysis of HUVEC cells, using . The picture on the right is blocked with the synthesized peptide.](http://images.novusbio.com/fullsize/FGF18-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06688-img0002.jpg "Immunocytochemistry/Immunofluorescence: FGF18 Antibody [NBP3-06688] - Immunofluorescence analysis of HUVEC cells, using . The picture on the right is blocked with the synthesized peptide.")
![Immunohistochemistry-Paraffin: FGF18 Antibody [NBP3-06688] - Human kidney tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.](http://images.novusbio.com/fullsize/FGF18-Antibody-Immunohistochemistry-Paraffin-NBP3-06688-img0001.jpg)
![Western Blot: ROBO1 Antibody (10E2-R) [NBP3-32913] - Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (NBP3-32913) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 ug/Lane) Lane 2: NIH/3T3 cell lysate (10 ug/Lane) Lane 3: Rat brain tissue lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: RAW264.7 cell lysate (10 ug/Lane) Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32913) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32913_mouse-robo1-mab-10e2-r-288202412442967.jpg "Western Blot: ROBO1 Antibody (10E2-R) [NBP3-32913] - Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (NBP3-32913) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 ug/Lane) Lane 2: NIH/3T3 cell lysate (10 ug/Lane) Lane 3: Rat brain tissue lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: RAW264.7 cell lysate (10 ug/Lane) Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32913) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ROBO1 Antibody (10E2-R) [NBP3-32913] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-ROBO1 antibody (NBP3-32913) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32913) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32913_mouse-robo1-mab-10e2-r-29820241416189.jpg)