PD-1 Proteins

156-B7) binds with a typical ED₅₀ of 0.15-0.75 µg/mL." class="big_lightbox"> 156-B7) binds with a typical ED₅₀ of 0.15-0.75 µg/mL." class="big_thumb" />

8986-PD) is immobilized at 1 µg/mL (100 µL/well), the concentration of recombinant human PD-L1 (B7-H1) Fc Chimera (156-B7) that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL." class="big_lightbox"> 8986-PD) is immobilized at 1 µg/mL (100 µL/well), the concentration of recombinant human PD-L1 (B7-H1) Fc Chimera (156-B7) that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL." class="big_thumb" />

156-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.70 uM." class="big_lightbox"> 156-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.70 uM." class="big_thumb" />

1224-PL) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.274 uM." class="big_lightbox"> 1224-PL) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.274 uM." class="big_thumb" />

AVI9049) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 3.2 nM and 13.2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.122 µM." class="big_lightbox"> AVI9049) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human PD-1 His protein (Catalog # 8986-PD) was measured at a concentration range between 3.2 nM and 13.2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.122 µM." class="big_thumb" />

1019-B7) binds with an ED₅₀ of 0.16-0.8 µg/mL." class="big_lightbox"> 1019-B7) binds with an ED₅₀ of 0.16-0.8 µg/mL." alt="When Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) is coated at 1 µg/mL, Recombinant Mouse B7-H1/PD-L1 Fc Chimera (Catalog # 1019-B7) binds with an ED₅₀ of 0.16-0.8 µg/mL." class="big_thumb" />

CP004), it binds to Recombinant Human PD-L1/B7-H1 Fc Chimera (Catalog # 156-B7) with an ED₅₀ of 1.2-7.2 ng/mL." class="big_lightbox"> CP004), it binds to Recombinant Human PD-L1/B7-H1 Fc Chimera (Catalog # 156-B7) with an ED₅₀ of 1.2-7.2 ng/mL." alt="When 500 ng/mL of Biotinylated Recombinant Human PD-1 His-tag Avi-tag protein is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human PD-L1/B7-H1 Fc Chimera (Catalog # 156-B7) with an ED₅₀ of 1.2-7.2 ng/mL." class="big_thumb" />

F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_lightbox"> F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_thumb" />

9075-PL) was measured at a concentration range between 5.07 nM and 2.60 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.173 µM." class="big_lightbox"> 9075-PL) was measured at a concentration range between 5.07 nM and 2.60 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.173 µM." class="big_thumb" />

156-B7) is coated at 0.5 µg/mL, 100 µL/well, Biotinylated Recombinant Human PD-1 Fc Chimera (Catalog # BT1086) binds with an ED₅₀ of 10.0-100 ng/mL." class="big_lightbox"> 156-B7) is coated at 0.5 µg/mL, 100 µL/well, Biotinylated Recombinant Human PD-1 Fc Chimera (Catalog # BT1086) binds with an ED₅₀ of 10.0-100 ng/mL." alt="When Recombinant Human B7-H1/PD-L1 Fc Chimera (156-B7) is coated at 0.5 µg/mL, 100 µL/well, Biotinylated Recombinant Human PD-1 Fc Chimera (Catalog # BT1086) binds with an ED₅₀ of 10.0-100 ng/mL." class="big_thumb" />

F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_lightbox"> F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_thumb" />

1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (Catalog # 9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM." class="big_lightbox"> 1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (Catalog # 9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM." alt="Recombinant Mouse PD-L1/B7-H1 Fc protein (1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (Catalog # 9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM." class="big_thumb" />

156-B7) is immobilized at 1 µg/mL, 100 µg/well, Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) binds with an ED₅₀ of 0.01-0.09 µg/mL." class="big_lightbox"> 156-B7) is immobilized at 1 µg/mL, 100 µg/well, Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) binds with an ED₅₀ of 0.01-0.09 µg/mL." alt="When Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) is immobilized at 1 µg/mL, 100 µg/well, Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) binds with an ED₅₀ of 0.01-0.09 µg/mL." class="big_thumb" />

9835-B7) is coated at 1 µg/mL, 100 µL/well, Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) binds with an ED₅₀ of 0.1-0.6 µg/mL." class="big_lightbox"> 9835-B7) is coated at 1 µg/mL, 100 µL/well, Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) binds with an ED₅₀ of 0.1-0.6 µg/mL." alt="When Recombinant Rat PD-L1/B7-H1 Fc Chimera (Catalog # 9835-B7) is coated at 1 µg/mL, 100 µL/well, Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) binds with an ED₅₀ of 0.1-0.6 µg/mL." class="big_thumb" />

10484-PD) is immobilized at 1 µg/mL (100 µL/ well), Recombinant Human PD-L1/B7‑H1 Fc Chimera (156-B7) that produces a 50% optimal binding response is found to be 0.03‑0.3 µg/mL" class="big_lightbox"> 10484-PD) is immobilized at 1 µg/mL (100 µL/ well), Recombinant Human PD-L1/B7‑H1 Fc Chimera (156-B7) that produces a 50% optimal binding response is found to be 0.03‑0.3 µg/mL" alt="When Recombinant Porcine PD‑1 Fc Chimera (10484-PD) is immobilized at 1 µg/mL (100 µL/ well), Recombinant Human PD-L1/B7‑H1 Fc Chimera (156-B7) that produces a 50% optimal binding response is found to be 0.03‑0.3 µg/mL" class="big_thumb" />

10484-PD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa and 120-150 kDa, respectively." class="big_lightbox"> 10484-PD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa and 120-150 kDa, respectively." class="big_thumb" />