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![Western Blot: Cyclin-O Antibody [NBP1-98285] - Titration: 1.0 ug/ml Positive Control: Fetal Heart.](http://images.novusbio.com/fullsize/Cyclin-O-Antibody-Western-Blot-NBP1-98285-img0002.jpg "Western Blot: Cyclin-O Antibody [NBP1-98285] - Titration: 1.0 ug/ml Positive Control: Fetal Heart.")
![Western Blot: Cyclin-O Overexpression Lysate (Adult Normal) [NBP2-06557] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclin-O.](http://images.novusbio.com/fullsize/Cyclin-O-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06557-img0001.jpg "Western Blot: Cyclin-O Overexpression Lysate (Adult Normal) [NBP2-06557] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclin-O.")
![Western Blot: Cyclin-O Antibody [H00010309-B01P-50ug] - Analysis of CCNO expression in transfected 293T cell line (H00010309-T01) by CCNO MaxPab polyclonal antibody. Lane 1: CCNO transfected lysate(38.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Cyclin-O-Antibody-Western-Blot-H00010309-B01P-50ug-img0001.jpg "Western Blot: Cyclin-O Antibody [H00010309-B01P-50ug] - Analysis of CCNO expression in transfected 293T cell line (H00010309-T01) by CCNO MaxPab polyclonal antibody. Lane 1: CCNO transfected lysate(38.5 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Cyclin L2 Antibody [NBP2-47497] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Cyclin-L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47497-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cyclin L2 Antibody [NBP2-47497] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Cyclin L2 Antibody [NBP2-47497] - Staining of human colon tissue. Shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclin-L2-Antibody-Immunohistochemistry-Paraffin-NBP2-47497-img0004.jpg)
![Western Blot: Cyclin L2 Antibody [NBP2-84745] - WB Suggested Anti-CCNL2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateCCNL2 is supported by BioGPS gene expression data to be expressed in Jurkat](http://images.novusbio.com/fullsize/Cyclin-L2-Antibody-Western-Blot-NBP2-84745-img0001.jpg "Western Blot: Cyclin L2 Antibody [NBP2-84745] - WB Suggested Anti-CCNL2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateCCNL2 is supported by BioGPS gene expression data to be expressed in Jurkat")
![Western Blot: Cyclin L2 Antibody [NB100-87009] - Whole cell lysate (50 ug) from HEK293T, K-562, HeLa, U2OS, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Cyclin L2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Cyclin-L2-Antibody-Western-Blot-NB100-87009-img0003.jpg "Western Blot: Cyclin L2 Antibody [NB100-87009] - Whole cell lysate (50 ug) from HEK293T, K-562, HeLa, U2OS, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Cyclin L2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Cyclin L2 Antibody [NB100-87009] - Detection of human Cyclin L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Cyclin L2 antibody NB100-87009 (lot 2) used for IP at 6 ug per reaction. Cyclin L2 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-Cyclin L2 antibody from Company B. For blotting immunoprecipitated Cyclin L2, NB100-87009 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Cyclin-L2-Antibody-Immunoprecipitation-NB100-87009-img0004.jpg)
![Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Simple-Western-NB500-106-img0008.jpg "Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunocytochemistry-Immunofluorescence-NB500-106-img0015.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunohistochemistry-Paraffin-NB500-106-img0007.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Western-Blot-NB500-106-img0006.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Western blot of PCNA levels across different stages of the cell cycle. U-2 OS cells were synchronised at G1/S with 2 mM thymidine an released for 10 hours for G2/M or re-arrested in G2 for extended duration with 10uM RO-3306. Antibody at 1:2000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Western-Blot-NB500-106-img0014.jpg)
![Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by Dr. Siegenthaler via product review.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunohistochemistry-Frozen-NB500-106-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - PC12 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunocytochemistry-Immunofluorescence-NB500-106-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunocytochemistry-Immunofluorescence-NB500-106-img0010.jpg)
![Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 um. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnana.2018.00029/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunohistochemistry-NB500-106-img0013.jpg)
![Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Flow-Cytometry-NB500-106-img0012.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Western-Blot-NB500-106-img0005.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Western-Blot-NB500-106-img0011.jpg)
![Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunohistochemistry-NB500-106-img0001.jpg)
![Chromatin Immunoprecipitation: PCNA Antibody (PC10) [NB500-106] - Events taking place during a transient telomere dysfunction. All strains were cdc13–1, incubated for 160 min at 36°C to induce telomere uncapping, followed by 90 min at 23°C to allow telomere re-capping. Nocodazole & BrdU were added to the cultures at time 0. (A) Dynamics of ssDNA loss in sub-telomeres. (B) BrdU incorporation in sub-telomeres, minus incorporation at ‘CEN’ (e.g. a centromere-proximal locus, in this case ERG26). (C–F) Dynamics of the protein association with sub-telomeres, measured for Rap1 & major DNA synthesis factors (indicated above each graph). ChIP (%) was calculated as the fraction of immunoprecipitated sub-telomeric DNA, minus the fraction precipitated at ‘CEN’ (G) Dynamics of ssDNA loss at YER188W. (H) BrdU incorporation at YER188W, minus incorporation at ‘CEN’. (I–L) The protein association with YER188W was analyzed as in C–F. (M) ssDNA at ‘CEN’. (N) BrdU incorporation at ‘CEN’. (O–R) Association of proteins with ‘CEN’. (S) Growth of serial dilution of wild-type (first row) & cdc13–1 cells with or without additional mutations (indicated on the left of each row) at temperatures indicated above each plate. The plate shown on the right was cycled three times between 4 h at 36°C (to accumulate ssDNA) & 4 h at 21°C (to re-synthesize DNA), followed by incubation at 21°C for another two days. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-106_mouse-monoclonal-pcna-antibody-pc10-310202416154647.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. (A–D) Immunofluorescence for PCNA (yellow) & DAPI staining (blue) on 30 μm-cryosections of E32–P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 μm. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. (E) Quantification of the VZ thickness of the E32–P1 tree shrew neocortex. (F) Quantification of the SVZ thickness of the E32–P1 tree shrew neocortex. (G) Quantification of the VZ & SVZ thickness of the E32–P1 tree shrew neocortex, expressed as percentage of the sum of VZ & SVZ. (H) Quantification of the iSVZ & oSVZ thickness of the E37 tree shrew neocortex, expressed as percentage of the sum of iSVZ & oSVZ. (E–H) Data represent mean ± SD & were obtained from two consecutive sections of two brains each. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29725291), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-106_mouse-monoclonal-pcna-antibody-pc10-31020241615468.jpg)