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![Western Blot: Rad51 Antibody (13E4) [NB100-499] - Detection of Rad51 in HeLa whole cell extract.](http://images.novusbio.com/fullsize/Rad51-Antibody-13E4-Western-Blot-NB100-499-img0005.jpg "Western Blot: Rad51 Antibody (13E4) [NB100-499] - Detection of Rad51 in HeLa whole cell extract.")
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0007.jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Jurkat cells with Rad51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Flow-Cytometry-NBP2-75640-img0001.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded human placenta tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded mouse testis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0006.jpg)
![Western Blot: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Rad51 on different lysates with Rabbit anti-Rad51 antibody at 1/1,000 dilution.Lane 1: Jurkat cell lysate (10 µg/Lane)Lane 2: NIH/3T3 cell lysate (10 µg/Lane)Lane 3: Mouse testis tissue lysate (20 µg/Lane)Predicted band size: 37 kDaObserved band size: 37 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-western-blot-2862024111350..jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of U-2 OS cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/100 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (ET1705-96) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunocytochemistry-immunofluorescence-2862024111534..jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of NIH/3T3 cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunocytochemistry-immunofluorescence-2862024111720..jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Mouse spleen tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-2862024111850..jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Human tonsil tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-286202411203..jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Mouse testis tissue with Rabbit anti-Rad51 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-2862024112111..jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (523713) [Unconjugated] [MAB99481] - Rad51 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse Rad51 Monoclonal Antibody (Catalog # MAB99481) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei.](http://images.novusbio.com/fullsize/Rad51-Antibody-523713-Unconjugated-Immunocytochemistry-Immunofluorescence-MAB99481-img0001.jpg "Immunocytochemistry/Immunofluorescence: Rad51 Antibody (523713) [Unconjugated] [MAB99481] - Rad51 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse Rad51 Monoclonal Antibody (Catalog # MAB99481) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei.")
![Immunohistochemistry-Paraffin: Rad51 Antibody (S04-3C6) [NBP3-14968] - Immunohistochemistry of paraffin-embedded Human tonsil using NBP3-14968 at dilution 1/50](http://images.novusbio.com/fullsize/nbp3-14968_rabbit-rad51-mab-s04-3c6-113202594522.jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (S04-3C6) [NBP3-14968] - Immunohistochemistry of paraffin-embedded Human tonsil using NBP3-14968 at dilution 1/50")
![Western Blot: Rad51 Antibody (S04-3C6) [NBP3-14968] - Western blot detection of A549, HL-60, U2OS cell lysates using NBP3-14968. Predicted band size: 37kDa. Observed band size: 37kDa.](http://images.novusbio.com/fullsize/nbp3-14968_rabbit-rad51-mab-s04-3c6-10320252347263.jpg)
![Western Blot: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Western Blot Analysis of HeLa, HepG2, K562, A431 and MCF-7 cell lysate using Rad51 Antibody (RAD51/2701).](http://images.novusbio.com/fullsize/Rad51-Antibody-RAD51-2701-Western-Blot-NBP2-79760-img0003.jpg "Western Blot: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Western Blot Analysis of HeLa, HepG2, K562, A431 and MCF-7 cell lysate using Rad51 Antibody (RAD51/2701).")
![Flow Cytometry: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Flow Cytometric Analysis of K562 cells using Rad51 Antibody (RAD51/2701)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Rad51-Antibody-RAD51-2701-Flow-Cytometry-NBP2-79760-img0001.jpg)
![Protein Array: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Rad51 Antibody (RAD51/2701) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Rad51-Antibody-RAD51-2701-Protein-Array-NBP2-79760-img0002.jpg)
![Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149] - Western blot analysis of extracts of various cell lines, using Rad51 Rabbit mAb (NBP3-16149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Rad51-Antibody-5S9B0-Western-Blot-NBP3-16149-img0001.jpg "Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149] - Western blot analysis of extracts of various cell lines, using Rad51 Rabbit mAb (NBP3-16149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Chromatin Immunoprecipitation: Rad51 Antibody (5S9B0) [NBP3-16149] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using Rad51 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16149_rabbit-rad51-mab-5s9b0-52202511361288.jpg)
![Western Blot: Rad51 Overexpression Lysate (Adult Normal) [NBP2-09787] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rad51.](http://images.novusbio.com/fullsize/Rad51-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09787-img0001.jpg "Western Blot: Rad51 Overexpression Lysate (Adult Normal) [NBP2-09787] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rad51.")
![ELISA: Human Rad51 ELISA Kit (Colorimetric) [NBP3-40314] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40314_human-rad51-elisa-kit-colorimetric-2110202415355081.png "ELISA: Human Rad51 ELISA Kit (Colorimetric) [NBP3-40314] - Standard Curve Reference")
![ELISA: Human Rad51 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40315] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40315_human-rad51-ready-to-use-elisa-kit-colorimetric-2110202415371363.png "ELISA: Human Rad51 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40315] - Standard Curve Reference")
![Western Blot: Rad51C Antibody (2H11/6) - BSA Free [NB100-177] - The expression of DNA damage-responsive proteins was measured by western blot analysis following treatment with olaparib and SAHA alone or in combination. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25888415/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rad51C-Antibody-2H11-6-BSA-Free-Western-Blot-NB100-177-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad51C Antibody (2H11/6) - BSA Free [NB100-177] - The expression of DNA damage-responsive proteins was measured by western blot analysis following treatment with olaparib and SAHA alone or in combination. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25888415/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Rad51C Antibody (2H11/6) [NB100-177] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Rad51C-Antibody-2H11-6-Flow-Cytometry-NB100-177-img0006.jpg)
![Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.](http://images.novusbio.com/fullsize/Rad51C-Antibody-2H11-6-Western-Blot-NB100-177-img0003.jpg)
![Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Analysis of RAD51c in 1) Hela WCE 2) HepG2 WCE 3) Cos-7 WCE 4) Hek293 WCE.](http://images.novusbio.com/fullsize/Rad51C-Antibody-2H11-6-Western-Blot-NB100-177-img0001.jpg)
![Simple Western: Rad51C Antibody (2H11/6) [NB100-177] - Lane view shows a specific band for Rad51C in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Rad51C-Antibody-2H11-6-Simple-Western-NB100-177-img0007.jpg)
