Zika Virus (H/PF/2013) Envelope Antibody (990801) [Unconjugated]

Western blot shows recombinant Zika Virus (H/PF/2013) Envelope (rvZIKV-E3). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Zika Virus (H/PF/2013) Envelope Monoclonal Antibody (Catalog # MAB10009) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Zika Virus (H/PF/2013) Envelope at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB, ELISA
• Clone: 990801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Zika Virus (H/PF/2013) Envelope Antibody (990801) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant Zika Virus (H/PF/2013) Envelope
  Leu590-Ser695
• Specificity: Detects Zika Virus (H/PF/2013) Envelope (E3) in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Zika Virus (H/PF/2013) Envelope Antibody (990801) [Unconjugated]

• Zika Virus (H/PF/2013) Envelope
• ZIKV_E
• ZIKV-E
• ZIKV-Env

Background

Zika Virus non-structural protein 1 (ZIKV NS1) is an approximately 48 kDa viral glycoprotein (1). Zika Virus is a mosquito-borne flavivirus and has been implicated an association with neonatal microcephaly and neurological disorders such as Guillain-Barré syndrome (2). ZIKV strain H/PF/2013 was isolated from human host.  ZIKV NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (1, 2). Infected cells secrete NS1 as a lipoprotein, which is involved in immune evasion and pathogenesis via interaction with components of the innate and adaptive immune systems (1, 2). Mature ZIKV NS1 contains 352 amino acids (aa) and has a hexameric conformation consisting of three dimers to form a symmetric barrel shape (1). It has high structural similarity to other flavivirus NS1 proteins, such as DENV and WNV (2, 3). Mature NS1 of ZIKV strain H/PF/2013 shares 98% aa sequence identity with NS1 of ZIKV strain MR 766 that was isolated from monkey host.

1. Song, H. et al. (2016) Nat. Struct. and Mol. Biol. 23:456.
2. Brown, WC. et al. (2016) Nat. Struct. and Mol. Biol. 23: 865.
3. Baronti, C. et al. (2014) Genome Announc. 2: e00500

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.