Zika Virus Envelope Antibody (GT871)

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-48648 as capture antibody at concentration of 5 ug/mL and GTX133326 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.

Non-infected (-) and infected (+) Vero cell extracts (15 ug, unboiled) were separated by gradient gel, and the membrane was blotted with Zika Virus Envelope antibody [GT871] (NBP3-48648) culture supernatant diluted at 1:50.

Zika Virus-PRVABC59 infected Vero cells were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika Virus Envelope antibody [GT871] (HRP) (NBP3-48648-01) diluted at 1:2000.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133326 as capture antibody at concentration of 5 ug/mL and NBP3-48648 as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.

Immunofluorescent analysis of non-infected and infected vero cells using Zika Virus Envelope antibody [GT871] (NBP3-48648). Green: Zika Virus Envelope antibody [GT871] (NBP3-48648) diluted at 1:500.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-48648 as capture antibody at concentration of 5 ug/mL and GTX133325 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.

Untreated (-) and treated (+) C6/36 cell extracts (15 ug) were separated by gradient gel, and the membrane was blotted with Zika Virus Envelope antibody [GT871] (NBP3-48648) diluted at 1:2000.

• Reactivity: V
• Applications: WB
• Clone: GT871
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Zika Virus Envelope Antibody (GT871) Summary

• Description: Novus Biologicals Mouse Zika Virus Envelope Antibody (GT871) (NBP3-48648) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika Virus Envelope (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.05M Tris, 0.15M NaCl, 1%BSA
• Preservative: 0.025% Proclin 300
• Purity: Protein A purified

Alternate Names for Zika Virus Envelope Antibody (GT871)

• polyprotein, partial

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.