Z-IE(OMe)TD(OMe)-FMK (Caspase 8 Inhibitor)

• Applications: Func

Z-IE(OMe)TD(OMe)-FMK (Caspase 8 Inhibitor) Summary

• Immunogen: Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK
  Z-D(OMe)E(OMe)VD(OMe)-FMK
• Details of Functionality: Molecular Weight: 654
  Formula: C30H43N4O11F
  Mass Spec: M+1=655.1
  TLC: EtOAc: 100%, Rf:0.3
  NMR: All functional groups are present

Applications/Dilutions

• Dilutions:
  • In vitro assay
  • In vivo assay
• Application Notes: 1. This inhibitor is designed as a methyl ester to facilitate cell permeability. If the intended use is on purified or recombinant enzymes, esterase should be added to generate the free carboxyl groups. Please contact us for more details.
  2. For in-vivo or in-vitro experiments extending longer than 12 hrs, fresh inhibitor may have to be added to culture medium or injected into animals.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Form:Yellow solid
  Make a stock solution of 5, 10 or 20 mM in high purity DMSO (>99.9%).

Alternate Names for Z-IE(OMe)TD(OMe)-FMK (Caspase 8 Inhibitor)

• C30H43N4O11F
• Z-IE(OMe)TD(OMe)-FMK
• Z-IETD-FMK
• Z-IETD-FMK [Z-Ile-Glu(OMe)-Thr-Asp(OMe)-FMK

Background

Members of the caspase family play key roles in apoptosis and inflammation. Z-IE(OMe)TD(OMe)-FMK is a cell-permeable caspase peptide inhibitor that irreversibly binds to the catalytic site of caspases proteases, and inhibits caspase mediated apoptosis by preventing the processing of pro-caspases to their active forms (reviewed in 1-3). Z-IETD-FMK was first described is an irreversible and cell permeable inhibitor of Caspase 8. The Z-IE(OMe)TD(OMe)-FMK peptide is O-methylated in the P1 and P3 positions providing enhanced stability and increased cell permeability. Z-IE(OMe)TD(OMe)-FMK (Z-IETD-FMK) is typically used in assays to inhibit apoptosis. Z-IETD-FMK has been used in many different types of apoptosis assays and published using a number of model system. Users may want to consult the literature for additional information regarding applications for Z-IETD D-FMK. Z-IETD-FMK is recommended as a search term for identifying references in PubMed using this peptide inhibito

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Publications for Z-IE(OMe)TD(OMe)-FMK Inhibitor (NBP2-29397)(1)

Publication using NBP2-29397

• Du Y, Taylor CG, Aukema HM, Zahradka P Regulation of docosahexaenoic acid-induced apoptosis of confluent endothelial cells: Contributions of MAPKs and caspases Biochimica et biophysica acta. Molecular and cell biology of lipids 2021-02-10 [PMID: 33578050]