wnvNS3 Protease Antibody (991935) [Unconjugated]

Western blot shows Recombinant Viral wnvNS3 Protease loaded at 10 µg and 2 µg. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29074) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for wnvNS3 Protease at approximately 32 kDa for the full length and 22 kDa for the C-terminal NS3 (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Viral wnvNS3 Protease protein was serially diluted 2-fold and captured by Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29074) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29073) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: V
• Applications: WB, ELISA
• Clone: 991935
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

wnvNS3 Protease Antibody (991935) [Unconjugated] Summary

• Immunogen: E. coli-derived Recombinant Viral wnvNS3 Protease
  sequences: rwnvNS2b 1-48, rwnvNS3 1-184
• Specificity: Detects Viral wnvNS3 Protease in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for wnvNS3 Protease Antibody (991935) [Unconjugated]

• wnvNS3 Protease

Background

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.